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Immune response of kuruma shrimp against microbial infections

Research Project

Project/Area Number 15H02462
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic life science
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

HIRONO IKUO  東京海洋大学, 学術研究院, 教授 (00270926)

Co-Investigator(Kenkyū-buntansha) 近藤 秀裕  東京海洋大学, 学術研究院, 教授 (20314635)
Project Period (FY) 2015-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥33,280,000 (Direct Cost: ¥25,600,000、Indirect Cost: ¥7,680,000)
Fiscal Year 2018: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2017: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2016: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2015: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Keywordsクルマエビ / WSSV / AHPND / ビブリオ病 / ホワイトスポット病 / 毒素タンパク質 / 病原微生物 / ホワイトスポットウイルス / 腸炎ビブリオ / 抗菌タンパク質 / 水産学 / マイクロアレイ / 免疫学 / ウイルス / 細菌 / 免疫・生体防御 / EMS/AHPND / 病原性微生物
Outline of Final Research Achievements

It was found that there was no relationship between the copy number of the toxin gene encoded by the virulence plasmid of Vibrio parahaemolyticus AHPND strain, which is a bacterium causing acute hepatopancreatic necrosis of shrimp. A few genes specifically expressed in shrimp which is resistant to the AHPND was identified.
It was revealed that the fossilized virus genome sequence of WSSV-like virus integrated in the prawn genome exists in multiple copies in various crustaceans. We found that some genes in the WSSV-like fossil virus genome were transcribed, suggesting that they may have a function.

Academic Significance and Societal Importance of the Research Achievements

世界中で猛威をふるったクルマエビ類の急性肝膵臓壊死病原のメカニズムを明らかにすることができたことは今後、本感染症の防除法開発に役立つと考えられる。
クルマエビ類のホワイトスポット病ウイルスは既知のウイルスと類似性がないことからユニークなウイルスであるとされていたが、甲殻類のゲノムを調べることにより、自然界には本ウイルス類似ウイルスが少なくとも過去には多種類存在していたことが明らかとなった。DNAゲノムであるホワイトスポット病ウイルスがマルチコピーで宿主のゲノムに組み込まれていることで、クルマエビゲノムの複雑さを理解する上で重要な知見になる。

Report

(5 results)
  • 2019 Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (51 results)

All 2019 2018 2017 2016 2015 Other

All Int'l Joint Research (4 results) Journal Article (16 results) (of which Int'l Joint Research: 13 results,  Peer Reviewed: 16 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (31 results) (of which Int'l Joint Research: 15 results,  Invited: 6 results)

  • [Int'l Joint Research] カセサート大学/タイ農務省水産局(タイ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] カセサート大学/タイ水産局(タイ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Mansoura University(エジプト)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] タイ水産局/カセサート大学(タイ)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Identification of an anti-lipopolysaccharide factor AV-R isoform (LvALF AV-R) related to Vp_PirAB-like toxin resistance in Litopenaeus vannamei.2019

    • Author(s)
      Tinwongger S, Thawonsuwan J, Kondo H, Hirono I.
    • Journal Title

      Fish Shellfish Immunol.

      Volume: 84 Pages: 178-188

    • DOI

      10.1016/j.fsi.2018.10.005

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Crustacean genome exploration reveals the evolutionary origin of white spot syndrome virus.2019

    • Author(s)
      Kawato S, Shitara A, Wang Y, Nozaki R, Kondo H, Hirono I.
    • Journal Title

      J Virol.

      Volume: 93 Issue: 3 Pages: 01144-18

    • DOI

      10.1128/jvi.01144-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] White spot syndrome virus (WSSV) suppresses penaeidin expression in Marsupenaeus japonicus hemocytes2018

    • Author(s)
      Zhang K, Koiwai K, Kondo H, Hirono I. (
    • Journal Title

      Fish Shellfish Immunol.

      Volume: 78 Pages: 233-237

    • DOI

      10.1016/j.fsi.2018.04.045

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Comparative sequence analysis of crustin isoform MjCRS7 and MjWFDC-like gene from kuruma shrimp Marsupenaeus japonicus shows variant of the WFDC domain2018

    • Author(s)
      Tandel GM, Hipolito SG, Kondo H, Hirono I.
    • Journal Title

      Infect Genet Evol.

      Volume: 64 Pages: 139-148

    • DOI

      10.1016/j.meegid.2018.06.008

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Draft Genome Sequence of Vibrio penaeicida Strain TUMSAT-NU1, Isolated from Diseased Shrimp in Japan2018

    • Author(s)
      Kawato Satoshi、Nozaki Reiko、Kondo Hidehiro、Hirono Ikuo
    • Journal Title

      Genome Announcements

      Volume: 6 Issue: 11

    • DOI

      10.1128/genomea.00153-18

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel white spot syndrome virus-induced gene (MjVIG1) from Marsupenaeus japonicus hemocytes2018

    • Author(s)
      Zhang Kehong、Koiwai Keiichiro、Kondo Hidehiro、Hirono Ikuo
    • Journal Title

      Fish & Shellfish Immunology

      Volume: 77 Pages: 46-52

    • DOI

      10.1016/j.fsi.2018.03.026

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gills specific type 2 crustin isoforms: Its molecular cloning and characterization from kuruma shrimp Marsupenaeus japonicus2018

    • Author(s)
      Tandel Gauravkumar M.、Kondo Hidehiro、Hirono Ikuo
    • Journal Title

      Developmental & Comparative Immunology

      Volume: 85 Pages: 25-30

    • DOI

      10.1016/j.dci.2018.03.018

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel viral responsive protein (MjVRP) from Marsupenaeus japonicus haemocytes is involved in white spot syndrome virus infection2017

    • Author(s)
      Elbahnaswy Samia、Koiwai Keiichiro、Zaki Viola H.、Shaheen Adel A.、Kondo Hidehiro、Hirono Ikuo
    • Journal Title

      Fish & Shellfish Immunology

      Volume: 70 Pages: 638-647

    • DOI

      10.1016/j.fsi.2017.09.045

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Recombinant PirA-like toxin protects shrimp against challenge with Vibrio parahaemolyticus, the aetiological agent of acute hepatopancreatic necrosis disease.2017

    • Author(s)
      Campa-Cdova AI, Len-Gallo AF, Romero-Maldonado A, Ibarra-Serrano AC, Rosales-Mendoza S, Hirono I, Angulo C.
    • Journal Title

      J Fish Dis.

      Volume: 印刷中 Issue: 11 Pages: 1725-1729

    • DOI

      10.1111/jfd.12625

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A rapid method for simultaneously diagnosing four shrimp diseases using PCR-DNA chromatography method2017

    • Author(s)
      Koiwai K、Kodera T、Thawonsuwan J、Kawase M、Kondo H、Hirono I
    • Journal Title

      Journal of Fish Diseases

      Volume: 41 Issue: 2 Pages: 395-399

    • DOI

      10.1111/jfd.12732

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Pathogen recognition of a novel C-type lectin from Marsupenaeus japonicus reveals the divergent sugar-binding specificity of QAP motif.2017

    • Author(s)
      Alenton RR, Koiwai K, Miyaguchi K, Kondo H, Hirono I.
    • Journal Title

      Scientific Reports

      Volume: 7 Pages: 45818-45818

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Extracellular trap formation in kuruma shrimp (Marsupenaeus japonicus) hemocytes is coupled with c-type lysozyme.2016

    • Author(s)
      Koiwai K, Alenton RR, Kondo H, Hirono I.
    • Journal Title

      Fish Shellfish Immunol.

      Volume: 52 Pages: 206-209

    • DOI

      10.1016/j.fsi.2016.03.039

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Characterization of a Kunitz-type protease inhibitor (MjKuPI) reveals the involvement of MjKuPI positive hemocytes in the immune responses of kuruma shrimp Marsupenaeus japonicus.2016

    • Author(s)
      Mai HN, Nguyen HT, Koiwai K, Kondo H, Hirono I.
    • Journal Title

      Dev Comp Immunol.

      Volume: 63 Pages: 121-127

    • DOI

      10.1016/j.dci.2016.05.022

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Virulence of Acute Hepatopancreatic Necrosis Disease PirAB-like Relies on Secreted Proteins Not on Gene Copy Number.2016

    • Author(s)
      Tinwongger S, Nochiri Y, Thawonsuwan J, Nozaki R, Kondo H, Awasthi SP, Hinenoya A, Yamasaki S, Hirono I.
    • Journal Title

      J Appl Microbiol.

      Volume: 121 Issue: 6 Pages: 1755-1765

    • DOI

      10.1111/jam.13256

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Gene silencing of VP9 gene impairs WSSV infectivity on Macrobrachium rosenbergii.2016

    • Author(s)
      Alenton RR, Kondo H, Hirono I, Maningas MB.
    • Journal Title

      Virus Res.

      Volume: 214 Pages: 65-70

    • DOI

      10.1016/j.virusres.2016.01.013

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Draft Genome Sequence of Non-Vibrio parahaemolyticus Acute Hepatopancreatic Necrosis Disease Strain KC13.17.5, Isolated from Diseased Shrimp in Vietnam.2015

    • Author(s)
      Kondo H, Van PT, Dang LT, Hirono I.
    • Journal Title

      Genome Announc.

      Volume: 3 Issue: 5

    • DOI

      10.1128/genomea.00978-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 沖縄県産クルマエビより見出された新規ビブリオ属細菌3株2019

    • Author(s)
      川戸 智・野崎玲子・近藤秀裕・廣野育生
    • Organizer
      平成31年度日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] A novel pattern recognition protein in Marsupenaeus japonicus hemocytes, MjPTX, binds to bacteria and promotes Ca2+-dependent agglutination in vitro2019

    • Author(s)
      Omar Adrianne Pan Alaman・Keiichiro Koiwai・Hidehiro Kondo・ Ikuo Hirono
    • Organizer
      A novel pattern recognition protein in Marsupenaeus japonicus hemocytes, MjPTX, binds to bacteria and promotes Ca2+-dependent agglutination in vitro
    • Related Report
      2018 Annual Research Report
  • [Presentation] PO0277 Crustacean Genome Exploration Reveals the Evolutionary Origin of Deadly Shrimp Virus2019

    • Author(s)
      Satoshi Kawato, Hidehiro Kondo and Ikuo Hirono
    • Organizer
      Plant and Animal Genome 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sequential characterization of four new gill-specific crustin isoforms from kuruma shrimp Marsupenaeus japonicus, and its gene expression profile in response to bacterial and viral challenge test2018

    • Author(s)
      Gauravkumar M. Tandel1、Hidehiro Kondo1、Ikuo Hirono1
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 日本産 WSSV 4 株の全ゲノム配列2018

    • Author(s)
      川戸 智・野崎玲子・近藤秀裕・廣野育生(海洋大)
    • Organizer
      平成30年度日本魚病学会秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 甲殻類ゲノム中の分子化石が明らかにする WSSV の起源と進化2018

    • Author(s)
      川戸 智,野崎 玲子,近藤 秀裕,廣野 育生
    • Organizer
      第20回マリンバイオテクノロジー学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 鶏卵卵黄抗体を用いたバナメイエビにおける急性肝膵臓壊死症防除法の開発2017

    • Author(s)
      中村 梨夏 , 近藤 秀裕 , 山本 雅一 , 廣野 育生
    • Organizer
      第19回マリンバイオテクノロジー学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Localization of MjPen-II in hemocytes of kuruma shrimp2017

    • Author(s)
      Kehong Zhang, Keiichiro Koiwai, Hidehiro Kondo, Ikuo Hirono
    • Organizer
      第19回マリンバイオテクノロジー学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Immune Defense of Shrimp Gills through Marsupenaeus japonicus Gill C-Type Lectin (MjGCTL)2017

    • Author(s)
      Rod Russel R. Alenton, Keiichiro Koiwai, Rika Nakamura, Jumroensri Thawonsuwan, Hidehiro Kondo and Ikuo Hirono
    • Organizer
      Diseases in Asian Aquaculture 10
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] What We Know and What We Have To Study for Understanding Fish and Shrimp Immune System?2017

    • Author(s)
      Ikuo Hirono
    • Organizer
      Diseases in Asian Aquaculture 10
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Transcriptome Analysis of Hepatopancreas and Stomach of AHPND Toxin-Resistant Litopenaeus vannamei2017

    • Author(s)
      Sasiwipa Tinwongger, Hidehiro Kondo and Ikuo Hirono
    • Organizer
      Diseases in Asian Aquaculture 10
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bacillus amyloliquefaciens TOA5001 株のクルマエビ類におけるプロバイオティクスとしての利用2017

    • Author(s)
      今泉健太郎・近藤秀裕・廣野育生・Jumroensri Thawonsuwan
    • Organizer
      平成29年度日本魚病学会秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] タカノケフサイソガニゲノムライブラリ中の WSSV 類似配列2017

    • Author(s)
      川戸 智・野崎玲子・近藤秀裕・廣野育生
    • Organizer
      平成29年度日本魚病学会秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Crustacean genomic fossils illuminate the evolutionary history of WSSV2017

    • Author(s)
      Satoshi Kawato, Reiko Nozaki, Hidehiro Kondo and Ikuo Hirono
    • Organizer
      JSFS85周年記念シンポジウム
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Expression and localization of MjPen-II in hemocytes of kuruma shrimp after WSSV challenge2017

    • Author(s)
      Kehong Zhang, Keiichiro Koiwai, Hidehiro Kondo and Ikuo Hirono
    • Organizer
      JSFS85周年記念シンポジウム
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of crustin isoform MjCRS8 and MjCRS9 from kuruma shrimp Marsupenaeus japonicas2017

    • Author(s)
      Gauravkumar M. Tandel, Hidehiro Kondo and Ikuo Hirono
    • Organizer
      JSFS85周年記念シンポジウム
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of genes associated with Vp_PirAB-like toxin resistance in Litopenaeus vannamei based on transcriptome analysis2017

    • Author(s)
      Sasiwipa Tinwongger, Jumroensri Thawonsuwan, Hidehiro Kondo and Ikuo Hirono
    • Organizer
      JSFS85周年記念シンポジウム
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 宿主ゲノム中の分子化石が明らかにするエビ病原ウイルスの起源と進化2017

    • Author(s)
      川戸智・野崎玲子・近藤秀裕.廣野育生
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Identification of genes associated with AHPND-resistance in Litopenaeus vannamei through transcriptome analysis2016

    • Author(s)
      Tinwongger S, Kondo H, and Hirono I.
    • Organizer
      平成28年度日本水産学会秋季大会
    • Place of Presentation
      近畿大学農学部
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Characterization of Vp_PirA-like and Vp_PirB-like in Vibrio parahaemolyticus causing-acute hepatopancreatic necrosis disease2016

    • Author(s)
      Tinwongger S, Nochiri Y, Nozaki R, Kondo H, and Hirono I.
    • Organizer
      第11回アジア水産学会
    • Place of Presentation
      バンコク
    • Year and Date
      2016-08-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Latest research on AHPND and measures to combat it2016

    • Author(s)
      Hirono I
    • Organizer
      FAO Second International Technical Seminar/Workshop on AHPND
    • Place of Presentation
      バンコク
    • Year and Date
      2016-06-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Hyper expansion of homologous genes of white spot syndrome virus (WSSV) in Penaeid shrimp genome2016

    • Author(s)
      Hirono I
    • Organizer
      016 International Symposium on Marine Genomics
    • Place of Presentation
      ソウル
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 十脚甲殻類におけるWSSV類似配列の探索2016

    • Author(s)
      川戸智、野崎玲子、近藤秀裕、廣野育生
    • Organizer
      第18回マリンバイオテクノロジー学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Latest Research on AHPND and other emerging shrimp diseases2016

    • Author(s)
      Hirono I.
    • Organizer
      ASEAN Regional Technical Consultation on EMS/AHPND and Other Transboundary Diseases for Improved Aquatic Animal Health Management in Southeast Asia
    • Place of Presentation
      フィリピン、マニラ
    • Year and Date
      2016-02-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Recent Advances of AHPND in Asia.2015

    • Author(s)
      Hirono I.
    • Organizer
      Annual Shrimp Congress in Phillipines
    • Place of Presentation
      フィリピン、ジェネラルサントス市
    • Year and Date
      2015-11-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] クルマエビ(Marsupenaeus japonicus)におけるVago様新規遺伝子に関する研究2015

    • Author(s)
      小祝敬一郎・近藤秀裕・廣野育生
    • Organizer
      平成27年度日本魚病学会秋季大会
    • Place of Presentation
      東京大学
    • Year and Date
      2015-09-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] Recent advances in AHPND caused by Vibrio species.2015

    • Author(s)
      Hirono I.
    • Organizer
      SEAFDEC conference 2015
    • Place of Presentation
      フィリピン、イロイロ市
    • Year and Date
      2015-07-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] dentifications of white spot syndrome virus homologues in kuruma shrimp, Marsupenaeus japonicus genome by Next generation Sequencing approach2015

    • Author(s)
      Aiko Shitara , Yuanyuan Wang, Keiichiro Koiwai, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono.
    • Organizer
      13th ISDCI Congress
    • Place of Presentation
      スペイン、ムルシア
    • Year and Date
      2015-06-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 密度勾配遠心法によって分画したクルマエビ血液細胞の顕微鏡観察および FACS 解析2015

    • Author(s)
      塩見玲菜、小祝敬一郎、近藤秀裕、廣野育生
    • Organizer
      第17回マリンバイオテクノロジー学会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2015-05-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] クルマエビが有しているホワイトスポットシンドロームウイルス類似遺伝子2015

    • Author(s)
      王媛媛、設楽愛子、近藤秀裕、廣野育生
    • Organizer
      第17回マリンバイオテクノロジー学会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2015-05-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 血管内皮増殖因子受容体(VEGFRlike-1および2)に対する抗体を用いた クルマエビ血球細胞の分類2015

    • Author(s)
      小祝敬一郎、塩見玲菜、近藤秀裕、廣野育生
    • Organizer
      第17回マリンバイオテクノロジー学会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2015-05-30
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2022-11-04  

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