| Budget Amount *help |
¥41,210,000 (Direct Cost: ¥31,700,000、Indirect Cost: ¥9,510,000)
Fiscal Year 2018: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2017: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2016: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2015: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
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| Outline of Final Research Achievements |
Microglia activation and astrogliosis are observed in the early stage of prion diseases. Therefore, glial activation are thought to be involved in the neurodegeneration, however, details of the mechanisms remain to be elucidated. In this study, first we analyzed the activation state of microgila and astrocyte in prion infected mice by RNA-sequencing. We also analyzed brain regions where neuronal loss was progressively observed in prion-infected mice and analyzed gene expression in the small region. Neuron-enriched genes were selected by bioinformatics and further analyses disclosed that expression of stress-induced transcriptional factor, ATF3, was induced in the lateral dorsal part of the thalamus where progressive neuronal loss was observed. The lateral dorsal part of the thalamus is one of the regions where intense glial activation is observed and thus, it is of interest to elucidate if glial activation induces STF3 induction in prion-infected neurons.
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