| Project/Area Number |
15H02482
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Insect science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Shimada Toru 東京大学, 大学院農学生命科学研究科(農学部), 教授 (20202111)
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| Co-Investigator(Kenkyū-buntansha) |
勝間 進 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (20378863)
木内 隆史 東京大学, 大学院農学生命科学研究科(農学部), 助教 (60622892)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥41,470,000 (Direct Cost: ¥31,900,000、Indirect Cost: ¥9,570,000)
Fiscal Year 2017: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2016: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2015: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
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| Keywords | 性決定 / 遺伝子量補正 / カイコ / チョウ目 / 性染色体 / 遺伝子ノックアウト / ゲノム編集 / 組換え抑制 / piRNA |
|---|
| Outline of Final Research Achievements |
We have revealed that the femaleness of the silkworm, Bombyx mori, is determined by a female-specific small RNA (Fem piRNA) derived from W chromosome, which suppresses expression of the male determining gene, Masc located on the Z chromosome. In this project, in order to clarify the molecular function of Masc, we performed experiments using genetically modified Masc proteins. As a result, we revealed that the zinc finger domain is not necessary, and the cysteine residue near the center is essential for sex determination. In addition, we found that the Masc ortholog in a bombycid, Trilocha varians, also determines the maleness. Furthermore, we clarified that the Masc knockout silkworms cause male-specific lethality at the embryonic stage.
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