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Cross talk among sex determination cascade, gene dosage compensation, and recombination supression in the silkworms

Research Project

Project/Area Number 15H02482
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Insect science
Research InstitutionThe University of Tokyo

Principal Investigator

Shimada Toru  東京大学, 大学院農学生命科学研究科(農学部), 教授 (20202111)

Co-Investigator(Kenkyū-buntansha) 勝間 進  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (20378863)
木内 隆史  東京大学, 大学院農学生命科学研究科(農学部), 助教 (60622892)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥41,470,000 (Direct Cost: ¥31,900,000、Indirect Cost: ¥9,570,000)
Fiscal Year 2017: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2016: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2015: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Keywords性決定 / 遺伝子量補正 / カイコ / チョウ目 / 性染色体 / 遺伝子ノックアウト / ゲノム編集 / 組換え抑制 / piRNA
Outline of Final Research Achievements

We have revealed that the femaleness of the silkworm, Bombyx mori, is determined by a female-specific small RNA (Fem piRNA) derived from W chromosome, which suppresses expression of the male determining gene, Masc located on the Z chromosome. In this project, in order to clarify the molecular function of Masc, we performed experiments using genetically modified Masc proteins. As a result, we revealed that the zinc finger domain is not necessary, and the cysteine residue near the center is essential for sex determination. In addition, we found that the Masc ortholog in a bombycid, Trilocha varians, also determines the maleness. Furthermore, we clarified that the Masc knockout silkworms cause male-specific lethality at the embryonic stage.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (18 results)

All 2018 2017 2016 2015 Other

All Journal Article (7 results) (of which Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Transcriptome profiling reveals infection strategy of an insect maculavirus2018

    • Author(s)
      Katsuma Susumu、Kawamoto Munetaka、Shoji Keisuke、Aizawa Takahiro、Kiuchi Takashi、Izumi Natsuko、Ogawa Moe、Mashiko Takaaki、Kawasaki Hideki、Sugano Sumio、Tomari Yukihide、Suzuki Yutaka、Iwanaga Masashi
    • Journal Title

      DNA Research

      Volume: 印刷中 Issue: 3 Pages: 277-286

    • DOI

      10.1093/dnares/dsx056

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Unique sex determination system in the silkworm, Bombyx mori : current status and beyond2018

    • Author(s)
      Susumu Katsuma, Takashi Kiuchi, Munetaka Kawamoto, Toshiaki Fujimoto, and Ken Sahara
    • Journal Title

      Proceedings of the Japan Academy, Series B, Physical and Biological Sciences(日本学士院欧文紀要), in press.

      Volume: in press

    • NAID

      130006733759

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] カイコ性決定遺伝子の同定とチョウ目昆虫における性操作技術の開発. (特集:農林水産業・食品産業科学技術研究推進事業の研究成果―基盤技術編―)2018

    • Author(s)
      勝間進
    • Journal Title

      JATAFFジャーナル

      Volume: 印刷中

    • NAID

      40021565298

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Artificial "ping-pong" cascade of PIWI-interacting RNA in silkworm cells2017

    • Author(s)
      Shoji K, Suzuki Y, Sugano S, Shimada T, Katsuma S.
    • Journal Title

      RNA

      Volume: 23 Issue: 14 Pages: 86-97

    • DOI

      10.1002/1873-3468.12246

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of a bipartite nuclear localization signal in the silkworm Masc protein2016

    • Author(s)
      Sugano Y, Kokusho R, Ueda M, Fujimoto M, Tsutsumi N, Shimada T, Kiuchi T, Katsuma S.
    • Journal Title

      FEBS Lett.

      Volume: 590 Issue: 1 Pages: 2256-2261

    • DOI

      10.1261/rna.058875.116

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Two conserved cysteine residues are required for the masculinizing activity of the silkworm Masc protein2015

    • Author(s)
      Katsuma S, Sugano Y, Kiuchi T, Shimada T.
    • Journal Title

      J. Biol. Chem.

      Volume: 290 Issue: 43 Pages: 26114-26124

    • DOI

      10.1074/jbc.m115.685362

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification and functional analysis of a Masculinizer orthologue in Trilocha varians (Lepidoptera: Bombycidae) play similar roles in the sex-determination cascade in Bombycidae2015

    • Author(s)
      Jung Lee, Takashi Kiuchi, Munetaka Kawamoto, Toru Shimada and Susumu Katsuma
    • Journal Title

      Insect Molecular Biology

      Volume: 24(5) Issue: 5 Pages: 561-569

    • DOI

      10.1111/imb.12181

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] エリサンにおけるゲノム編集の現状(2017年度版).2018

    • Author(s)
      李允求・木内隆史・勝間進・嶋田透
    • Organizer
      第62回 日本応用動物昆虫学会大会. 小集会W14: カイコと近縁蛾類におけるゲノム編集と系統保存-NBRPプロジェクトの取組み
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] カイコのPIWIタンパク質遺伝子BmAgo3のノックアウトが生体へ及ぼす影響2017

    • Author(s)
      木内隆史・庄司佳祐・川本宗孝・菅野 純夫・嶋田 透・鈴木 穣・勝間 進
    • Organizer
      日本蚕糸学会第87回大会(平成29年度蚕糸・昆虫機能利用学術講演会)
    • Place of Presentation
      農林水産技術会議事務局 筑波産学連携支援センター(茨城県つくば市)
    • Year and Date
      2017-03-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of Masculinizer homolog in Samia cynthia ricini2017

    • Author(s)
      Lee J, Kiuchi T, Shimada T, Katsuma S.
    • Organizer
      The 5th Asia-Pacific Congress of Sericulture and Insect Biotechnology (APSERI 2017 Bangkok)
    • Place of Presentation
      Maruay Garden Hotel, Bangkok, Thailand
    • Year and Date
      2017-02-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genome editing in the masculinizing gene of the silkworm Bombyx mori2016

    • Author(s)
      Kiuchi T , Sugano Y, Shimada T, Katsuma S.
    • Organizer
      XXV International Congress of Entomology. Symposium: Genomics and Genome Engineering in the Silkworm (Organizers: Takashi Kiuchi and Kallare Arunkumar)
    • Place of Presentation
      Orange County Convention Center, Orlando, Florida 32819, U. S. A.
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] カイコの雄化誘導遺伝子Mascの進化に迫る2016

    • Author(s)
      李允求・木内隆史・川本宗孝・鈴木穣・菅野純夫・笠原雅弘・西山智明・柴田朋子・重信秀治・山口勝司・嶋田透
    • Organizer
      日本昆虫学会第76回大会・第60回日本応用動物昆虫学会合同大会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス(大阪府堺市)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] エリサンSamia cynthia riciniのMascホモログは遺伝子量補償に関与するが、性決定には関与しない2016

    • Author(s)
      李允求・木内隆史・川本宗孝・西山智明・鈴木穣・菅野純夫・笠原雅弘・重信秀治・山口勝司・嶋田透・勝間進
    • Organizer
      日本蚕糸学会第86回大会(平成28年度蚕糸・昆虫機能利用学術講演会)
    • Place of Presentation
      京都工芸繊維大学松ヶ崎キャンパス(京都府京都市)
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] CRISPR/Cas9システムによるカイコのオス化遺伝子Mascの機能解析2016

    • Author(s)
      木内 隆史・菅野 雄大・嶋田 透・勝間 進
    • Organizer
      日本蚕糸学会第86回大会(平成28年度蚕糸・昆虫機能利用学術講演会)
    • Place of Presentation
      京都工芸繊維大学松ヶ崎キャンパス(京都府京都市)
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] カイコ雄化因子Mascの雄化能に必須なアミ ノ酸残基の同定2015

    • Author(s)
      勝間進・木内隆史・菅野雄大・嶋田透
    • Organizer
      日本蚕糸学会第85回大会(平成27年度蚕糸・昆虫機能利用学術講演会)
    • Place of Presentation
      北海道大学大学院農学研究院(北海道札幌市)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] カイコ雄化因子Mascの細胞内局在解析および標的の探索2015

    • Author(s)
      菅野雄大・庄司佳祐・古賀光・木内隆史・嶋田透・勝間進
    • Organizer
      日本蚕糸学会第85回大会(平成27年度蚕糸・昆虫機能利用学術講演会)
    • Place of Presentation
      北海道大学大学院農学研究院(北海道札幌市)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] イチジクカサンTrilocha varians の性決定機構の解明と、カイコガ科における性決定機構の進化に関する考察.2015

    • Author(s)
      李允求・木内隆史・嶋田透・勝間進
    • Organizer
      日本蚕糸学会第85回大会(平成27年度蚕糸・昆虫機能利用学術講演会)
    • Place of Presentation
      北海道大学大学院農学研究院(北海道札幌市)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Remarks] 主要な研究論文の紹介

    • URL

      http://papilio.ab.a.u-tokyo.ac.jp/igb/ja/Research_katsuma.html

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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