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Molecular mechanism of sex-specific defenses in a social parasitoid

Research Project

Project/Area Number 15H02483
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Insect science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

IWABUCHI KIKUO  東京農工大学, (連合)農学研究科(研究院), 名誉教授 (00203399)

Co-Investigator(Kenkyū-buntansha) 吉村 仁  静岡大学, 創造科学技術大学院, 教授 (10291957)
佐藤 令一  東京農工大学, (連合)農学研究科(研究院), 教授 (30235428)
松浦 健二  京都大学, 農学研究科, 教授 (40379821)
野村 昌史  千葉大学, 大学院園芸学研究科, 准教授 (50228368)
天竺桂 弘子  東京農工大学, (連合)農学研究科(研究院), 教授 (80434190)
Project Period (FY) 2015-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥40,040,000 (Direct Cost: ¥30,800,000、Indirect Cost: ¥9,240,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2016: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2015: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Keywords社会性昆虫 / 寄生蜂 / 生殖 / 多胚生殖 / 性差
Outline of Final Research Achievements

In polyembryonic parasitoids, it was revealed that sexual differences exist not only in attacking and increasing in soldier larvae but also in humoral toxic factors. As a result of transcriptome analysis and mass spectrometry of polyembryos, proteins involved in proteolysis were identified as factors secreted by the polyembryos. Regarding the mechanism of female-specific increase in soldier larvae, the venom injected by the heterospecific parasitoid into the same host during egg laying induces both germ cell death and secondary vasa down-regulation, or both of cell death and vasa down-regulation. That is, it was clarified that the increase in soldier larvae is caused by the fate conversion of the embryo.

Academic Significance and Societal Importance of the Research Achievements

多胚性寄生蜂では、多胚生殖の発達と並行して不妊の兵隊幼虫による社会性を進化させてきた。申請者らは外敵に対するこれら防御機構に性的差異が存在することをこれまでに明らかにしてきた。本研究はその分子基盤を明らかにすることで、社会性の発達、ならびに生殖とは関係のない場面での性的差異の発達についての理解を深化させた。これらの知見は論文のほか、「Polyembryonic Insects」として上梓し、広く社会に学術的意義を伝えた。

Report

(6 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (22 results)

All 2020 2019 2018 2017 2016 2015

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 9 results,  Acknowledgement Compliant: 2 results) Presentation (11 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Analysis of molecular mechanism for acceleration of polyembryony using gene functional annotation pipeline in Copidosoma floridanum.2020

    • Author(s)
      Sakamoto, T., Nishiko, M., Bono, H., Nakazato, T., Yoshimura, J., Tabunoki, H., Iwabuchi, K.
    • Journal Title

      BMC Genomics

      Volume: 21 Issue: 1 Pages: 152-152

    • DOI

      10.1186/s12864-020-6559-3

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Apoptosis-mediated vasa down-regulation controls developmental transformation in Japanese Copidosoma floridanum female soldiers.2019

    • Author(s)
      Ohno,H., Sakamoto,T., Okochi, R., Nishiko, M., Sasaki, S., Bono, H., Tabunoki, H., Iwabuchi, K.
    • Journal Title

      Developmental Biology

      Volume: 456 Issue: 2 Pages: 226-233

    • DOI

      10.1016/j.ydbio.2019.09.005

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mass killing by female soldier larvae is adaptive for the killed male larvae in a polyembryonic wasp2019

    • Author(s)
      Otsuki Takahiro、Uka Daisuke、Ito Hiromu、Ichinose Genki、Nii Momoka、Morita Satoru、Sakamoto Takuma、Nishiko Maaya、Tabunoki Hiroko、Kobayashi Kazuya、Matsuura Kenji、Iwabuchi Kikuo、Yoshimura Jin
    • Journal Title

      Scientific Reports

      Volume: 9 (7357) Issue: 1 Pages: 1-7

    • DOI

      10.1038/s41598-019-43643-3

    • NAID

      120006764368

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Superoxide dismutase down-regulation and the oxidative stress is required to initiate pupation in Bombyx mori.2019

    • Author(s)
      2.Nojima Y, Bono H, Yokoyama T, Iwabuchi K, Sato R, Arai K, Tabunoki H.
    • Journal Title

      Sci Rep.

      Volume: 9 Issue: 1 Pages: 14693-14693

    • DOI

      10.1038/s41598-019-51163-3

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparative analysis of seven types of superoxide dismutases for their ability to respond to oxidative stress in Bombyx mori.2019

    • Author(s)
      Kobayashi, Y., Nojima, Y., Sakamoto, T., Iwabuchi, K., Nakazato, T., Bono, H., Toyoda, A., Fujiyama, A., Kanost, MR., Tabunoki, H.
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 2170-2170

    • DOI

      10.1038/s41598-018-38384-8

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of heat shock and ambient temperature on female soldier production in a polyembryonic parasitic wasp2019

    • Author(s)
      Takano Y, Ono H, Sakamoto T, Yoshimura J, Iwabuchi K.
    • Journal Title

      Physiological Entomology

      Volume: - Issue: 2 Pages: 1-7

    • DOI

      10.1111/phen.12281

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of functional enolase genes of the silkworm Bombyx mori from public databases with a combination of dry and wet bench processes2017

    • Author(s)
      Kikuchi, A., Nakazato, T., Ito, K., Nojima, Y., Yokoyama, T., Iwabuchi, K., Bono, H., Toyoda, A., Fujiyama, A., Sato, R., Tabunoki, H.
    • Journal Title

      BMC Genomics

      Volume: 18 Issue: 1 Pages: 83-83

    • DOI

      10.1186/s12864-016-3455-y

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sexual complementarity between host humoral toxicity and soldier caste in a polyembryonic wasp2016

    • Author(s)
      Uka,D., Sakamoto, T., Yoshimura, J., Iwabuchi, K.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 1-7

    • DOI

      10.1038/srep29336

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Lazy workers are necessary for long-term sustainability in insect societies.2016

    • Author(s)
      Eisuke Hasegawa, Yasunori Ishii, Koichiro Tada, Kazuya Kobayashi, Jin Yoshimura
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 20846-20846

    • DOI

      10.1038/srep20846

    • NAID

      120005743059

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Comparison between the amount of environmental change and the amount of transcriptome change2015

    • Author(s)
      Norichika Ogata,Toshinori Kozaki, Takeshi Yokoyama,Tamako Hata,Kikuo Iwabuchi
    • Journal Title

      PLOS ONE

      Volume: なし Issue: 12 Pages: e0144822-e0144822

    • DOI

      10.1371/journal.pone.0144822

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 多胚性寄生蜂における寄主胚侵入に関わる分子メカニズムの探索2020

    • Author(s)
      坂本卓磨、坊農秀雅、天竺桂弘子、岩淵喜久男
    • Organizer
      第64回日本応用動物昆虫学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] キンウワバトビコバチの血縁に応じた性比戦略2020

    • Author(s)
      石井宗一郎、岩淵喜久男、野村昌史
    • Organizer
      第64回日本応用動物昆虫学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] キンウワバトビコバチの交尾行動に血縁関係が及ぼす影響に関して2019

    • Author(s)
      石井宗一郎、岩淵喜久男、野村昌史
    • Organizer
      日本応用動物昆虫学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 多胚性寄生蜂の胚子期トランスクリプトームに基づくヒトホモログのパスウェイ解析2019

    • Author(s)
      坂本琢磨、坊農秀雅、天竺桂弘子、岩淵喜久男
    • Organizer
      日本応用動物昆虫学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 多胚性寄生蜂のカースト多型2018

    • Author(s)
      岩淵喜久男
    • Organizer
      日本生態学会第65回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 多胚性寄生蜂における多胚形成期遺伝子の発現動態解析2018

    • Author(s)
      坂本卓磨、天竺桂弘子、岩淵喜久男
    • Organizer
      第62回日本応用動物昆虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Comparative transcriptome analyses of male and female polyembryos of the polyembryonic parasitic wasp2016

    • Author(s)
      Sakamoto, T., Ono, H., Iwabuchi, K.
    • Organizer
      the 22nd International Congress of Zoology and the 87th meeting of Zoology Society of Japan
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Vasa expression in morulae and polyembryos of a polyembryonic parasitic wasp Copidosoma floridanum treated with competitive parasitic wasp venom in vitro2016

    • Author(s)
      Ono,H., Sakamoto, T., Iwabuchi, K.
    • Organizer
      the 22nd International Congress of Zoology and the 87th meeting of Zoology Society of Japan
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Transcriptome analysis of a polyembryonic parasitoid Copidosoma floridanum2016

    • Author(s)
      Sakamoto, T., Ono, H., Iwabuchi, K.
    • Organizer
      XXV International Congress of Entomology
    • Place of Presentation
      Orland, 米国
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 昆虫類の寄生蜂による宿主免疫攪乱機構2016

    • Author(s)
      岩淵喜久男
    • Organizer
      日本比較免疫学会
    • Place of Presentation
      東京医科歯科大学(東京都・文京区)
    • Year and Date
      2016-08-20
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 多胚性寄生蜂のトランスクリプトーム解析2015

    • Author(s)
      坂本卓磨、岩淵喜久男
    • Organizer
      日本動物学会 第86回大会
    • Place of Presentation
      新潟コンベンションセンター(新潟市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Book] Polyembryonic Insects2019

    • Author(s)
      Iwabuchi, K.
    • Total Pages
      199
    • Publisher
      Springer
    • ISBN
      9789811509575
    • Related Report
      2019 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2022-11-04  

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