| Budget Amount *help |
¥47,190,000 (Direct Cost: ¥36,300,000、Indirect Cost: ¥10,890,000)
Fiscal Year 2017: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2016: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2015: ¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000)
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| Outline of Final Research Achievements |
One of the major obstacles in intracellular targeting using antibodies is their limited release from endosomes into the cytosol. We have developed an approach to deliver biomacromolecules into cells by using endosomolytic peptides derived from the cationic and membrane-lytic spider venom peptide M-lycotoxin. The delivery peptides were developed by introducing a glutamic acid into the hydrophobic face (L17E) to M-lycotoxin, enabling a marked cytosolic liberation of proteins including antibodies from endosomes. The predominant membrane-perturbation mechanism of this peptide is assumed to be the preferential disruption of negatively charged membranes (endosomal membranes) over neutral membranes (plasma membranes), and the endosomolytic peptide promotes the uptake by inducing macropinocytosis.
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