Cell biological studies on membrane lipid distribution and dynamics
| Project/Area Number |
15H02500
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大崎 雄樹 名古屋大学, 医学系研究科, 准教授 (00378027)
辻 琢磨 名古屋大学, 医学系研究科, 助教 (40725628)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO HAYASHI 東京大学, 大学院医学系研究科, 准教授 (80551283)
TAGUCHI TOMOHIKO 東京大学, 大学院薬学研究科, 准教授 (10300881)
TAKATORI SHO 東京大学, 大学院薬学研究科, 助教 (80624361)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥46,540,000 (Direct Cost: ¥35,800,000、Indirect Cost: ¥10,740,000)
Fiscal Year 2017: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2016: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2015: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
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| Keywords | 膜脂質 / 脂肪滴 / オートファジー / 核膜 / 肝細胞 / 凍結割断レプリカ法 / 脂質ドメイン / リポファジー / 電子顕微鏡 |
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| Outline of Final Research Achievements |
Electron microscopic methods to label phosphatidylinositol 3,5-bisphosphate, phosphatidylinositol 3,4-bisphosphate, and phosphatidylserine by quick-freezing and freeze-fracture replica labeling were established and distribution of respective phospholipids were defined at the nanometer scale. Involvement of PML-II in formation of nuclear lipid droplets was found and close relationship between nuclear lipid droplets and PML nuclear body as well as nucleoplasmic reticulum, which is an extension of the nuclear envelope, was shown. Microautophagy of lipid droplets that occurs in budding yeast at stationary phase and in acute nitrogen starvation was shown to proceed in a raft-like membrane domain of the vacuole membrane and Niemann-Pick type C proteins were found to play a critical role in transportation of sterol to generate the membrane domain.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes.2016
Author(s)
Makino A, Hullin-Matsuda F, Murate M, Abe M, Tomishige N, Fukuda M, Yamashita S, Fujimoto T, Vidal H, Lagarde M, Delton I, Kobayashi T.
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Journal Title
Mol Biol Cell
Volume: 27
Issue: 21
Pages: 3293-3304
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] ROR1 sustains caveolae and survival signaling as a scaffold of cavin-1 and caveolin-12016
Author(s)
Yamaguchi T, Lu C, Yanagisawa K, Can Lu, Usukura J, Cheng J, Hotta N, Shimada Y, Ida R, Isomura H, Suzuki M, Fujimoto T, Takahashi T
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Journal Title
Nat Commun
Volume: 7
Issue: 1
Pages: 10060-10060
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay2015
Author(s)
Takeda H, Ogasawara T, Ozawa T, Muraguchi A, Jih PJ, Morishita R, Uchigashima M, Watanabe M, Fujimoto T, Iwasaki T, Endo Y, Sawasaki T
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Journal Title
Sci Rep
Volume: 5
Issue: 1
Pages: 11333-11333
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Deficiency in the Lipid Exporter ABCA1 Impairs Retrograde Sterol Movement and Disrupts Sterol Sensing at the Endoplasmic Reticulum2015
Author(s)
Yamauchi Y, Iwamoto N, Rogers MA, Abe-Dohmae S, Fujimoto T, Chang CC, Ishigami M, Kishimoto T, Kobayashi T, Ueda K, Furukawa K, Chang TY, Yokoyama S.
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Journal Title
J Biol Chem
Volume: 290
Issue: 39
Pages: 23464-23477
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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