| Budget Amount *help |
¥42,120,000 (Direct Cost: ¥32,400,000、Indirect Cost: ¥9,720,000)
Fiscal Year 2017: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2016: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
Fiscal Year 2015: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
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| Outline of Final Research Achievements |
We transplanted feces of ulcerative colitis (UC) and primary sclerosing cholangitis (PSC) patients to germ-free (GF) mice (UC mice or PSC/UC mice). The ratio of Th17 cells was significantly increased in the liver of the PSC/UC mice compared to the UC mice. Klebsiella pneumonia (Kp), Proteus mirabillus, Enterococcus gallineum were frequently detected in feces of PSC+UC patient. We inoculated these three bacteria to GF mice (3 microbe mix mice; 3 mix mice). Th17 cells were accumulated in the liver. We also confirmed 3mix mice induce severe liver damage after DDC treatment compare to UC mice. Kp is found in the lamina propria of Kp mono associated mice. Kp induced epithelial cell apoptosis by using co-culture of Kp and human colon epithelial cells. As above, we revealed Kp destroys intestinal epithelial cells and induces "leaky gut" in the pathological condition of PSC.
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