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Application of iPS cell technology to lung regeneration and development of bioresource for elucidating the mechanisms of lung diseases

Research Project

Project/Area Number 15H02537
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionKyoto University

Principal Investigator

Michiaki Mishima  京都大学, 医学研究科, 名誉教授 (60190625)

Co-Investigator(Kenkyū-buntansha) 瀬山 邦明  順天堂大学, 医学部, 先任准教授 (10226681)
半田 知宏  京都大学, 医学研究科, 助教 (10432395)
平井 豊博  京都大学, 医学研究科, 教授 (20359805)
金 永学  京都大学, 医学研究科, 助教 (20456883)
佐藤 篤靖  京都大学, 医学研究科, 助教 (30706677)
佐藤 晋  京都大学, 医学(系)研究科(研究院), その他 (40378691)
伊藤 功朗  京都大学, 医学研究科, 助教 (40447975)
長崎 忠雄  京都大学, 医学研究科, 医員 (40747862)
堀田 秋津  京都大学, iPS細胞研究所, 特定拠点講師 (50578002)
小熊 毅  京都大学, 医学研究科, 特定助教 (50601324)
後藤 慎平  京都大学, 医学研究科, 特定准教授 (50747219)
室 繁郎  京都大学, 医学研究科, 准教授 (60344454)
松本 久子  京都大学, 医学研究科, 講師 (60359809)
小笹 裕晃  京都大学, 医学研究科, 特定助教 (80572015)
永井 宏樹  京都大学, 医学研究科, 特定助教 (80711605)
陳 和夫  京都大学, 医学研究科, 特定教授 (90197640)
浅香 勲  京都大学, iPS細胞研究所, 教授 (10543639)
谷澤 公伸  京都大学, 医学研究科, 客員研究員 (20639140)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥41,470,000 (Direct Cost: ¥31,900,000、Indirect Cost: ¥9,570,000)
Fiscal Year 2017: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2016: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2015: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Keywords分子細胞呼吸学 / iPS細胞 / 肺臓器再生 / 疾患モデリング / 分子細胞呼吸器学
Outline of Final Research Achievements

It has been troublesome to differentiate various human iPS cell lines into lung lineage cells, because different cell lines have different capacity of differentiation. In this project, we have established the robust and efficient methods of generating functional airway and alveolar epithelial cells from various human iPS cell lines by using Carboxypeptidase M-based lung progenitor cell sorting and organoid culture techniques. Moreover, we achieved long-term culture of human iPS cell-derived alveolar type II cells. Then, we applied these methods to animal transplantation studies and human disease modeling such as drug-induced alveolar epithelial injury, airway diseases, pulmonary fibrosis and lung cancer.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (28 results)

All 2017 2016 2015

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (19 results) (of which Int'l Joint Research: 3 results) Book (1 results)

  • [Journal Article] Long-term expansion of alveolar stem cells derived from human iPS cells in organoids2017

    • Author(s)
      Yamamoto Yuki、Gotoh Shimpei、Korogi Yohei、Seki Masahide、Konishi Satoshi、Ikeo Satoshi、Sone Naoyuki、Nagasaki Tadao、Matsumoto Hisako、Muro Shigeo、Ito Isao、Hirai Toyohiro、Kohno Takashi、Suzuki Yutaka、Mishima Michiaki
    • Journal Title

      Nature Methods

      Volume: 14 Issue: 11 Pages: 1097-1106

    • DOI

      10.1038/nmeth.4448

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ヒトiPS細胞から気道上皮細胞への分化促進2016

    • Author(s)
      小西 聡史, 後藤 慎平
    • Journal Title

      呼吸と循環

      Volume: 64 Pages: 785-789

    • NAID

      40020930524

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Directed induction of functional multi-ciliated cells in proximal airway epithelial spheroids from human pluripotent stem cells.2016

    • Author(s)
      Konishi S, Gotoh S, Tateishi K, Yamamoto Y, Korogi Y, Matsumoto H, Muro S, Hirai T, Ito I, Tsukita S, Mishima M.
    • Journal Title

      Stem Cell Reports

      Volume: 6 Issue: 1 Pages: 18-25

    • DOI

      10.1016/j.stemcr.2015.11.010

    • NAID

      120005745633

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Longitudinal shape irregularity of airway lumen assessed by CT in patients with bronchial asthma and COPD.2015

    • Author(s)
      Oguma T, Hirai T, Fukui M, Tanabe N, Marumo S, Nakamura H, Ito H, Sato S, Niimi A, Ito I, Matsumoto
    • Journal Title

      Thorax

      Volume: 70(8) Issue: 8 Pages: 719-24

    • DOI

      10.1136/thoraxjnl-2014-206651

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Matrix metalloproteinase-10: a novel biomarker for idiopathic pulmonary fibrosis.2015

    • Author(s)
      Sokai A, Handa T, Tanizawa K, Oga T, Uno K, Tsuruyama T, Kubo T, Ikezoe K, Nakatsuka Y, Tanimura K, Muro S, Hirai T, Nagai S, Chin K, Mishima M.
    • Journal Title

      Respir Res.

      Volume: 16 Issue: 1 Pages: 120-120

    • DOI

      10.1186/s12931-015-0280-9

    • NAID

      120005774669

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Mechanical Stimulation by Postnasal Drip Evokes Cough.2015

    • Author(s)
      Iwata T, Ito I, Niimi A, Ikegami K, Marumo S, Tanabe N, Nakaji H, Kanemitsu Y, Matsumoto H, Kamei J, Setou M, Mishima M.
    • Journal Title

      PLos One

      Volume: 10 Issue: 11 Pages: e0141823-e0141823

    • DOI

      10.1371/journal.pone.0141823

    • NAID

      120005749926

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A toll-like receptor 3 single nucleotide polymorphism in Japanese patients with sarcoidosis.2015

    • Author(s)
      Ikezoe K, Handa T, Tanizawa K, Kubo T, Ito I, Sokai A, Nakatsuka Y, Nagai S, Izumi T, Mishima M
    • Journal Title

      Tissue Antigens

      Volume: 85 Issue: 3 Pages: 204-208

    • DOI

      10.1111/tan.12535

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ヒト多能性幹細胞からⅡ型肺胞上皮細胞への分化促進と単離2015

    • Author(s)
      後藤 慎平, 三嶋 理晃
    • Journal Title

      呼吸と循環

      Volume: 63 Pages: 651-655

    • NAID

      40020519047

    • Related Report
      2015 Annual Research Report
  • [Presentation] In vitro disease modeling of alveolar phenotype caused by AP3B1 deficiency using human induced pluripotent stem cells.2017

    • Author(s)
      Korogi Y, Gotoh S, Yamamoto Y, Konishi S, Nagasaki T, Sone N, Ikeo S, Matsumoto H, Muro S, Ito I, Asaka I, Hotta A, Hirai T
    • Organizer
      Stem Cell Research 15th Annual Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒトiPS細胞を用いたⅡ型肺胞上皮細胞の量産化を目指して2017

    • Author(s)
      山本佑樹、後藤慎平、興梠陽平、小西聡史、池尾聡、曽根尚之、玉井浩二、平井豊博
    • Organizer
      第53回日本肺サーファクタント関連(界面)医学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒトiPS細胞由来肺胞オルガノイドによるHermansky-Pudlak症候群2型のモデリング2017

    • Author(s)
      興梠陽平、後藤慎平、山本佑樹、池尾 聡、曽根尚之、小西聡史、玉井浩二、平井豊博
    • Organizer
      第53回日本肺サーファクタント関連(界面)医学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Long-term expansion of alveolar stem cells derived from human iPS cells by forming organoids2017

    • Author(s)
      山本佑樹、後藤慎平、興梠陽平、関真秀、小西聡史、池尾聡、曽根尚之、長崎忠雄、松本久子、室繁郎、伊藤功朗、平井豊博、河野隆志、鈴木穣、三嶋理晃
    • Organizer
      CiRA 2017 International Symposium
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒト多能性幹細胞由来の呼吸器上皮細胞の有用性2017

    • Author(s)
      後藤慎平
    • Organizer
      第40回日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Induction of functional lung epithelial cells from human pluripotent stem cells2017

    • Author(s)
      後藤慎平
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒト多能性幹細胞から分化した気道繊毛上皮の有用性2016

    • Author(s)
      後藤慎平
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒトiPS細胞の呼吸器研究への応用2016

    • Author(s)
      後藤慎平
    • Organizer
      第29回日本動物実験代替法学会
    • Place of Presentation
      九州大学百年講堂
    • Year and Date
      2016-11-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Long-term culture of alveolar epithelial type 2 cells derived from human induced pluripotent stem cells.2016

    • Author(s)
      山本佑樹、後藤慎平、興梠陽平、小西聡史、長崎忠雄、松本久子、室繁郎、平井豊博、伊藤功朗、三嶋理晃
    • Organizer
      International Society for Stem Cell Research 14th Annual Meeting
    • Place of Presentation
      Samfrancisco, USA
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒトiPS細胞から機能的な気道線毛上皮細胞への分化誘導2016

    • Author(s)
      小西聡史、後藤慎平、立石和博、山本佑樹、興梠陽平、長崎忠雄、松本久子、室繁郎、平井豊博、伊藤功朗、月田早智子、三嶋理晃
    • Organizer
      第56回 日本呼吸器学会学術講演会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2016-04-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Generation of lung epithelial cells from human iPS cells2016

    • Author(s)
      伊藤功朗
    • Organizer
      第56回日本呼吸器学会学術講演会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2016-04-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒトiPS細胞から呼吸器上皮細胞への分化誘導技術2016

    • Author(s)
      後藤慎平
    • Organizer
      第56回日本呼吸器学会学術講演会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2016-04-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] iPS細胞を用いた肺疾患研究と治療戦略2016

    • Author(s)
      伊藤功朗
    • Organizer
      第37回日本呼吸器学会生涯教育講演会
    • Place of Presentation
      京都市国際交流会館
    • Year and Date
      2016-04-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒトiPS細胞から気道線毛上皮細胞への分化誘導2015

    • Author(s)
      小西聡史、後藤慎平、立石和博、山本佑樹、興梠陽平、長崎忠雄、松本久子、室繁郎、平井豊博、伊藤功朗、月田早智子、三嶋理晃
    • Organizer
      第38回 日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒト多能性幹細胞から呼吸器上皮細胞への分化誘導2015

    • Author(s)
      後藤慎平、伊藤功朗、興梠陽平、山本佑樹、小西聡史、長崎忠雄、松本久子、佐藤篤靖、室繁郎、平井豊博、長船健二、浅香勲、瀬山邦明、三嶋理晃
    • Organizer
      第15回 日本細胞生物学会
    • Place of Presentation
      タワーホール船堀
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] Generation of ciliated airway epithelial cells from human induced pluripotent stem cells2015

    • Author(s)
      小西聡史、後藤慎平、山本佑樹、興梠陽平、長崎忠雄、松本久子、室繁郎、平井豊博、伊藤功朗、三嶋理晃
    • Organizer
      ISSCR 2015 annual meeting
    • Place of Presentation
      Stockholm, Sweden
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 遺伝性呼吸器疾患の疾患特異的iPS細胞樹立2015

    • Author(s)
      後藤慎平、伊藤功朗、興梠陽平、山本佑樹、小西聡史、長崎忠雄、松本久子、佐藤篤靖、室繁郎、平井豊博、長船健二、浅香勲、瀬山邦明、三嶋理晃
    • Organizer
      第55回日本呼吸器学会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2015-04-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒトiPS細胞からⅡ型肺胞上皮細胞への分化誘導効率の改善2015

    • Author(s)
      山本佑樹、後藤慎平、興梠陽平、小西聡史、長崎忠雄、松本久子、室繁郎、平井豊博、伊藤功朗、三嶋理晃
    • Organizer
      第55回日本呼吸器学会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2015-04-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒトiPS細胞から気道繊毛上皮前駆細胞への効率の良い分化誘導法の確立2015

    • Author(s)
      小西聡史、後藤慎平、山本佑樹、興梠陽平、長崎忠雄、松本久子、室繁郎、平井豊博、伊藤功朗、三嶋理晃
    • Organizer
      第55回日本呼吸器学会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2015-04-17
    • Related Report
      2015 Annual Research Report
  • [Book] Annual Review 2016 呼吸器 [永井厚志・巽浩一郎・桑野和善・高橋和久(編)]  「ヒトiPS細胞から気道上皮細胞への分化促進」の項2016

    • Author(s)
      後藤慎平(分担執筆)
    • Publisher
      中外医学社
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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