| Budget Amount *help |
¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2017: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2016: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2015: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
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| Outline of Final Research Achievements |
B cells constitute a complex system of antigen-presenting cells (APCs) and exist as distinct subsets that differ in their lineage affiliation, surface molecule expression, and biological function, potentially regulating the immune response. In this study, we investigated the immune-regulatory roles of murine B cell subsets as regulatory APCs targeting alloreactive T cells. Anti-Balb/c T cell responses and serum levels of anti-Balb/c antibodies in the recipients of peritoneal cavity (PerC) B cells containing MHC class II+ CD80+ CD86+ PD-L1+ PD-L2+ cells were significantly lower than those in the recipients of splenic B cells. Pre-incubated with anti-PD-L1/PD-L2 mAbs prior to injection abrogated their immune-regulatory effects on anti-Balb/c T cells. The inoculation with Balb/c PerC B cells significantly prolonged the survival of subsequently grafted Balb/c hearts in B6 mouse recipients. Thus, the PerC PD-L1/PD-L2 B-1a cells suppress T cells responding to allostimulation.
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