Development of a novel desensitization/immune-regulatory method by use of multi-potent suppressor B cells to induce immune-tolerance in allogeneic organ transplantation
| Project/Area Number |
15H02555
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
OHDAN Hideki 広島大学, 医歯薬保健学研究科(医), 教授 (10363061)
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| Co-Investigator(Kenkyū-buntansha) |
小林 孝彰 愛知医科大学, 医学部, 教授 (70314010)
田中 友加 広島大学, 医歯薬保健学研究科(医), 准教授 (90432666)
尾上 隆司 独立行政法人国立病院機構(呉医療センター臨床研究部), 免疫応用科学研究室, 室長 (90549809)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2017: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2016: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2015: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
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| Keywords | 臓器移植 / 免疫寛容 / 脱感作 / B細胞 / 移植免疫 / 寛容誘導 / 拒絶反応 |
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| Outline of Final Research Achievements |
B cells constitute a complex system of antigen-presenting cells (APCs) and exist as distinct subsets that differ in their lineage affiliation, surface molecule expression, and biological function, potentially regulating the immune response. In this study, we investigated the immune-regulatory roles of murine B cell subsets as regulatory APCs targeting alloreactive T cells. Anti-Balb/c T cell responses and serum levels of anti-Balb/c antibodies in the recipients of peritoneal cavity (PerC) B cells containing MHC class II+ CD80+ CD86+ PD-L1+ PD-L2+ cells were significantly lower than those in the recipients of splenic B cells. Pre-incubated with anti-PD-L1/PD-L2 mAbs prior to injection abrogated their immune-regulatory effects on anti-Balb/c T cells. The inoculation with Balb/c PerC B cells significantly prolonged the survival of subsequently grafted Balb/c hearts in B6 mouse recipients. Thus, the PerC PD-L1/PD-L2 B-1a cells suppress T cells responding to allostimulation.
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Report
(4 results)
Research Products
(15 results)
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[Presentation] FcγRの遺伝子多型が及ぼすリツキシマブB細胞抑制効果への影響2015
Author(s)
坂井寛, 田中友加, 清水誠一, 井手健太郎, 広瀬貴行, 田中飛鳥, 矢野琢也, 佐伯吉弘, 谷峰直樹, 大平真裕, 田原裕之, 石山宏平, 尾上隆司, 田代裕尊, 大段秀樹.
Organizer
第51回日本移植学会総会
Place of Presentation
熊本
Year and Date
2015-10-01
Related Report
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