Developing DNA / RNA molecular devices for constructing a new generation of artificial cells.
| Project/Area Number |
15H02774
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Life / Health / Medical informatics
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
齊藤 博英 京都大学, iPS細胞研究所, 教授 (20423014)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
|
|---|
| Keywords | 人工細胞 / 分子ロボティクス / RNAナノテクノロジー / DNAナノテクノロジー / DNAコンピュータ / 細胞融合 / RNP / バイオテクノロジー |
|---|
| Outline of Final Research Achievements |
In this project we developed functional molecular devices using DNA / RNA. It would lead to the realization of new generation artificial cells showing new functions in the future. We performed design and functional evaluation of artificial molecular device group and developed a method to actually introduce the device into the cell. As a result, we achieved the goal of constructing artificial RNP (complex of RNA and protein) nanostructures in mammalian cells, and controling the cell fate. In addition, artificial cells that switch between motive-state and stationary-state were constructed using DNA complex molecular system (molecular clutch) that controls on / off linkage with lipid membrane. In addition, all the results of the molecular devices we constructed were also published in the journal papers. The results of this project will be used as a basis for constructing a new generation of artificial cells.
|
Report
(4 results)
Research Products
(55 results)
-
-
[Journal Article] DNA cytoskeleton for stabilizing artificial cells2017
Author(s)
Kurokawa Chikako、Fujiwara Kei、Morita Masamune、Kawamata Ibuki、Kawagishi Yui、Sakai Atsushi、Murayama Yoshihiro、Nomura Shin-ichiro M.、Murata Satoshi、Takinoue Masahiro、Yanagisawa Miho
-
Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 114
Issue: 28
Pages: 7228-7233
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Revolving Vernier Mechanism Controls Size of Linear Homomultimer2017
Author(s)
Takeo Uchida, Keita Abe, Yuma Endo, Shosei Ichiseki, Satoru Akita, Shiyun Liu, Sho Aradachi, Masataka Saito, Akihiko Fukuchi, Taiyo Kikkawa, Theo Dammaretz, Ibuki Kawamata, Yuki Suzuki, Shin-ichiro M. Nomura, Satoshi Murata
-
Journal Title
Small
Volume: 13
Issue: 41
Pages: 1702158-1702158
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
[Journal Article] Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate.2017
Author(s)
Tomonori Shibata, Yoshihiko Fujita, Hirohisa Ohno, Yuki Suzuki, Karin Hayashi, Kaoru R. Komatsu, Shunsuke Kawasaki, Kumi Hidaka, Shin Yonehara, Hiroshi Sugiyama, Masayuki Endo and Hirohide Saito
-
Journal Title
Nature Communications
Volume: 8
Issue: 1
Pages: 540-540
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
[Journal Article] Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches.2015
Author(s)
Miki K, Endo K, Takahashi S, Funakoshi S, Takei I, Katayama S, Toyoda T, Kotaka M, Takaki T, Umeda M, Okubo C, Nishikawa M, Oishi A, Narita M, Miyashita I, Asano K, Hayashi K, Osafune K, Yamanaka S, Saito H, Yoshida Y.
-
Journal Title
Cell Stem Cell.
Volume: 16(6)
Issue: 6
Pages: 699-711
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
