A new light energy flow model connecting from sunlight to marine ecosystem

• Project/Area Number: 15H02800
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental dynamic analysis
• Research Institution: The University of Tokyo
• Principal Investigator: Yoshizawa Susumu 東京大学, 大気海洋研究所, 准教授 (00553108)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000); Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 海洋微生物 / ロドプシン / 微生物生態 / 光エネルギー / 海洋細菌 / 海洋生態 / 細菌 / ゲノム

Outline of Final Research Achievements

In this research, the following results were obtained from genomic and metagenomic data analysis and experiments using molecular biological methods.
1. The light-adaptation strategies of marine bacteria possessing proteorhodopsin were clarified. 2. The structural analysis of Na+ and Cl- pumping rhodopsin (KR2 and NM-R3) found in marine bacteria revealed these ion transport mechanisms. 3. The functions of unknown microbial rhodopsins present in various environments were clarified.

Academic Significance and Societal Importance of the Research Achievements

地球表層の約７割を占める海洋で、どのような生物が、どのような機構を用いて太陽から光エネルギーを受容しているのか？一般的には、海洋表層に生息する植物プランクトンが光合成により光エネルギーを利用していると答えるのが常識である。しかしながら、近年の遺伝子解析手法の発展に伴い多くの海洋細菌がロドプシンを用いて太陽光からエネルギーを受け取っていることが分かってきた。本研究では、生態系を支えるエネルギー基盤である太陽光が、ロドプシンを通してどのように海洋生態系に流れ込むのかを明らかにするため、海洋細菌の持つロドプシンの網羅的探索および機能解析を実施し、複数の新規ロドプシンの機能を明らかにした。

Research Products

• [Int'l Joint Research] University of Hawaii(米国)
• [Int'l Joint Research] ハワイ大学(米国)
• [Journal Article] Solar-panel and parasol strategies shape the proteorhodopsin distribution pattern in marine Flavobacteriia (2018)
  • Author(s): Kumagai Yohei、Yoshizawa Susumu、Nakajima Yu、Watanabe Mai、Fukunaga Tsukasa、Ogura Yoshitoshi、Hayashi Tetsuya、Oshima Kenshiro、Hattori Masahira、Ikeuchi Masahiko、Kogure Kazuhiro、DeLong Edward F.、Iwasaki Wataru
  • Journal Title: The ISME Journal Volume: in press Issue: 5 Pages: 1329-1343
  • DOI: 10.1038/s41396-018-0058-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Proteorhodopsin variability and distribution in the North Pacific Subtropical Gyre (2018)
  • Author(s): Olson Daniel K.、Yoshizawa Susumu、Boeuf Dominique、Iwasaki Wataru、DeLong Edward F.
  • Journal Title: The ISME Journal Volume: 12 Issue: 4 Pages: 1047-1060
  • DOI: 10.1038/s41396-018-0074-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Spectroscopic characteristics of Rubricoccus marinus xenorhodopsin (RmXeR) and a putative model for its inward H+ transport mechanism (2018)
  • Author(s): Inoue Saki、Yoshizawa Susumu、Nakajima Yu、Kojima Keiichi、Tsukamoto Takashi、Kikukawa Takashi、Sudo Yuki
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 20 Issue: 5 Pages: 3172-3183
  • DOI: 10.1039/c7cp05033j
  • Peer Reviewed
• [Journal Article] Implications for the light-driven chloride ion transport mechanism of Nonlabens marinus rhodopsin 3 by its photochemical characteristics (2017)
  • Author(s): *Tsukamoto T, Yoshizawa S, Kikukawa T, Demura M, *Sudo Y.
  • Journal Title: J. Phys. Chem. B Volume: 121 Issue: 9 Pages: 2027-2038
  • DOI: 10.1021/acs.jpcb.6b11101
  • Peer Reviewed / Open Access
• [Journal Article] An inhibitory role of Arg-84 in anion channelrhodopsin-2 expressed in Escherichia coli (2017)
  • Author(s): Doi S, Tsukamoto T, Yoshizawa S, & *Sudo Y.
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 41879-41879
  • DOI: 10.1038/srep41879
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A phylogenetically distinctive and extremely heat stable light-driven proton pump from the eubacterium Rubrobacter xylanophilus DSM 9941T (2017)
  • Author(s): Kanehara K, Yoshizawa S, Tsukamoto T, *Sudo Y.
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 44427-44427
  • DOI: 10.1038/srep44427
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Draft Genome Sequences of Tersicoccus phoenicis DSM 30849T, Isolated from a Cleanroom for Spacecraft Assembly, and Tersicoccus sp. Strain Bi-70, Isolated from a Freshwater Lake (2017)
  • Author(s): Yu Nakajima, Susumu Yoshizawa, Keiji Nakamura, Yoshitoshi Ogura, Tetsuya Hayashi and Kazuhiro Kogure
  • Journal Title: Genome announcements Volume: 5 Issue: 13
  • DOI: 10.1128/genomea.00079-17
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural mechanism for light-driven transport by a new type of chloride ion pump, Nonlabens marinus rhodopsin-3 (2016)
  • Author(s): Toshiaki Hosaka, Susumu Yoshizawa, Yu Nakajima, Noboru Ohsawa, Masakatsu Hato, Edward F. DeLong, Kazuhiro Kogure, Shigeyuki Yokoyama, Tomomi Kimura-Someya, Wataru Iwasaki and Mikako Shirouzu
  • Journal Title: Journal of Biological Chemistry Volume: 291 Issue: 34 Pages: 17488-17495
  • DOI: 10.1074/jbc.m116.728220
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 微生物型ロドプシンの多様な機能 (2016)
  • Author(s): 吉澤 晋
  • Journal Title: 光合成研究 Volume: 26 Pages: 149-154
  • Peer Reviewed
• [Journal Article] Functional and Photochemical Characterization of a Light-Driven Proton Pump from the Gammaproteobacterium Pantoea vagans. (2016)
  • Author(s): Sudo Y, Yoshizawa S.
  • Journal Title: Photochem. Photobiol. Volume: in press Issue: 3 Pages: 420-427
  • DOI: 10.1111/php.12585
  • Peer Reviewed / Open Access
• [Journal Article] Structural basis for Na+ transport mechanism by a light-driven Na+ pump (2015)
  • Author(s): H. E. Kato, K. Inoue, R. Abe-Yoshizumi, Y. Kato, H. Ono, M. Konno, S. Hososhima, T. Ishizuka, M. R. Hoque, H. Kunitomo, J. Ito, S. Yoshizawa, K. Yamashita, M. Takemoto, T. Nishizawa, R. Taniguchi, K. Kogure, A. D. Maturana, Y. Iino, H. Yawo, R. Ishitani, H. Kandori*, O. Nureki*
  • Journal Title: Nature Volume: 未定 Issue: 7550 Pages: 48-53
  • DOI: 10.1038/nature14322
  • Peer Reviewed / Open Access
• [Presentation] A “photo”heterotrophic lifestyle of rhodopsin-containing bacteria (2017)
  • Author(s): Susumu Yoshizawa
  • Organizer: Ocean University of China-The University of Tokyo Marine Microbiology Workshop
  • Int'l Joint Research / Invited
• [Presentation] Microbial rhodopsin: a photoreceptor protein found in any environment where water exists and light reaches (2017)
  • Author(s): Susumu Yoshizawa
  • Organizer: Joint Symposium on Ocean, Coastal, and Atmospheric Sciences
  • Int'l Joint Research / Invited
• [Presentation] 微生物型ロドプシン：光利用機構の多様性は我々に何を語るのか？ (2017)
  • Author(s): 吉澤 晋
  • Organizer: 生命科学系学会合同年次大会(ConBio2017)
  • Invited
• [Presentation] NGSが解き明かす海洋微生物の新しい光エネルギー利用機構 (2017)
  • Author(s): 吉澤 晋
  • Organizer: NGS現場の会第五回研究会
  • Invited
• [Presentation] 海洋微生物のロドプシンを用いた新しい光エネルギー利用機構 (2017)
  • Author(s): 吉澤 晋
  • Organizer: 第１７回東京大学生命科学シンポジウム
  • Invited
• [Presentation] ウイルスによるロドプシン遺伝子の水平伝播 (2016)
  • Author(s): 吉澤 晋
  • Organizer: 第31回日本微生物生態学会
  • Place of Presentation: 横須賀市文化会館（神奈川県・横須賀市）
  • Year and Date: 2016-11-23
  • Invited
• [Presentation] ミッション：機能未知遺伝子の正体を暴け！ (2016)
  • Author(s): 吉澤 晋
  • Organizer: ゲノム時代の海洋生物学の展望
  • Place of Presentation: 大気海洋研究所講堂（千葉県・柏市）
  • Year and Date: 2016-11-02
• [Presentation] A new light-driven H+ pumping rhodopsin is plant-associated Gammaproteobacterium “Pantoea vagans” (2016)
  • Author(s): Susumu Yoshizawa and Yuki Sudo
  • Organizer: 16th International Society for Microbial Ecology
  • Place of Presentation: Montreal (Canada)
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] 光あるところにロドプシンあり：水圏微生物の新しい光エネルギー利用機構 (2016)
  • Author(s): 吉澤 晋
  • Organizer: 第一回水圏微生物研究フォーラム
  • Place of Presentation: 大気海洋研究所講堂（千葉県・柏市）
  • Year and Date: 2016-08-09
• [Presentation] ロドプシンで太陽エネルギーを利用する-光駆動型イオンポンプ- (2016)
  • Author(s): 吉澤 晋
  • Organizer: 第８９回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪府・大阪市）
  • Year and Date: 2016-03-23
  • Invited
• [Presentation] プロテオロドプシン保有発光細菌における発光量の調節 (2015)
  • Author(s): 中島悠, 吉澤, 木暮一啓
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピアアイランド（兵庫県・神戸市）
  • Year and Date: 2015-12-01
• [Presentation] プロテオロドプシンを持つ海洋細菌の有光層への適応に寄与する２つの遺伝子、SbtAとDUF2237 (2015)
  • Author(s): 熊谷 洋平, 吉澤 晋, 福永 津嵩, 渡辺 麻衣, 池内 昌彦, 小椋 義俊, 林 哲也, 木暮 一啓, 岩崎 渉
  • Organizer: 第30回日本微生物生態学会
  • Place of Presentation: 土浦亀城プラザ（茨城県・土浦市）
  • Year and Date: 2015-10-22
• [Presentation] ゲノム駆動型の未知遺伝子の機能解析 -プロテオロドプシンとDUF2237を例として- (2015)
  • Author(s): 熊谷 洋平, 吉澤 晋, 木暮 一啓, 岩崎 渉
  • Organizer: 生命情報科学若手の会第７回研究会
  • Place of Presentation: 慶應義塾大学鶴岡タウンキャンパス（山形県・鶴岡市）
  • Year and Date: 2015-10-01
• [Presentation] Physiological advantage of bacteira possessing multiple rhodopsins (2015)
  • Author(s): Yu Nakajima, Susumu Yoshizawa, Kazuhiro Kogure
  • Organizer: 14th Aquatic Microbial Ecology (SAME14)
  • Place of Presentation: Uppsala, Sweden
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] プロテオロドプシンを持つ海洋性フラボバクテリアのゲノム比較から、新規の光利用関連遺伝子を探る (2015)
  • Author(s): 熊谷 洋平, 吉澤 晋, 福永 津嵩, 渡辺 麻衣, 池内 昌彦, 小椋 義俊, 林 哲也, 木暮 一啓, 岩崎 渉
  • Organizer: NGS現場の会第四回研究会
  • Place of Presentation: つくば国際会議場（茨城県・つくば市）
  • Year and Date: 2015-07-01
• [Remarks] 光を利用するか、それとも避けるか？ ～海洋細菌の二種類の光適応戦略の解明～
  • URL: http://www.aori.u-tokyo.ac.jp/research/news/2018/20180206.html
• [Remarks] 海洋細菌由来新規ロドプシンによる、光によってナトリウムイオンを細胞外へと運び出す仕組みの解明
  • URL: http://www.aori.u-tokyo.ac.jp/research/topics/2015/20150515.html