Regulation of radiation-induced DNA damage repair through chromatin remodeling

• Project/Area Number: 15H02819
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Kyoto University
• Principal Investigator: Kobayashi Junya 京都大学, 放射線生物研究センター, 准教授 (30301302)
• Co-Investigator(Kenkyū-buntansha): 林 幾江 広島大学, 医歯薬保健学研究科(歯), 助教 (00346503)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: DNA損傷 / 放射線 / DNA修復 / クロマチン / クロマチンリモデリング / 複製ストレス

Outline of Final Research Achievements

As the importance of chromatin remodeling factors has been reported in radiation-induced DNA double-strand breaks (DSBs) damage responses, we investigated to identify chromatin remodeling factors, which could function in DSB damage responses,using immunoprecipitation/proteomics analysis. As a results, we identified several candidates of chromatin remodeling-related factors. Among of them, we found that nucleolin could function in ATR activation and homologous recombination, which suggests that nucelolin might be an important factor for replication stress-related cellular responses.

Research Products

• [Journal Article] Structural alteration of DNA induced by viral protein R of HIV-1 triggers the DNA damage response (2018)
  • Author(s): Iijima Kenta、Kobayashi Junya、Ishizaka Yukihito
  • Journal Title: Retrovirology Volume: 15 Issue: 1
  • DOI: 10.1186/s12977-018-0391-8
  • Peer Reviewed / Open Access
• [Journal Article] Potential relationship between the biological effects of low-dose irradiation and mitochondrial ROS production (2018)
  • Author(s): Kawamura Kasumi、Qi Fei、Kobayashi Junya
  • Journal Title: Journal of Radiation Research Volume: - Issue: suppl_2 Pages: ii91-ii97
  • DOI: 10.1093/jrr/rrx091
  • NAID: 120006647309
  • Peer Reviewed / Open Access
• [Journal Article] ATM-mediated mitochondrial damage response triggered by nuclear DNA damage in normal human lung fibroblasts. (2017)
  • Author(s): Shimura T, Sasatani M, Kawai H, Kamiya K, Kobayashi J, Komatsu K, Kunugita N.
  • Journal Title: Cell Cycle. Volume: 16(24) Issue: 24 Pages: 2345-2354
  • DOI: 10.1080/15384101.2017.1387697
  • Peer Reviewed / Open Access
• [Journal Article] NBS1 is regulated by two kind of mechanisms; ATM-dependent complex formation with MRE11 and RAD50 and cell cycle dependent-degradation of protein. (2017)
  • Author(s): Zhou H, Kawamura K, Yanagihara H, Kobayashi J, Zhang-Akiyama Q.
  • Journal Title: J Radiat Res. Volume: 59 Issue: 4 Pages: 1-8
  • DOI: 10.1093/jrr/rrx014
  • NAID: 120006647308
  • Peer Reviewed / Open Access
• [Journal Article] Chronic irradiation with low-dose-rate 137Cs-γ rays inhibits NGF-induced neurite extension of PC12 cells via Ca2+/calmodulin-dependent kinase II activation. (2017)
  • Author(s): Katoh S, Kobayashi J, Umeda T, Kobayashi Y, Nobuo I and Suzuki T
  • Journal Title: J Radiat Res Volume: 58 Issue: 6 Pages: 809-815
  • DOI: 10.1093/jrr/rrx032
  • Peer Reviewed / Open Access
• [Journal Article] A comparison of radiation-induced mitochondrial damage between neural progenitor stem cells and differentiated cells. (2017)
  • Author(s): Shimura T, Sasatani M, Kawai H, Kamiya K, Kobayashi J, Komatsu K, Kunugita N.
  • Journal Title: Cell Cycle. Volume: 16 Issue: 6 Pages: 565-573
  • DOI: 10.1080/15384101.2017.1284716
  • Peer Reviewed / Open Access
• [Journal Article] An approach to elucidate NBS1 function in DNA repair using frequent nonsynonymous polymorphism in wild medaka (Oryzias Latipes) populations (2017)
  • Author(s): K. Igarashi, J. Kobayashi, T. Katsumura, Y. Urushibara, K. Hida, T. Watanabe-Asaka, H. Oota, S. Oda and H. Mitani
  • Journal Title: PLOS ONE Volume: 12(1) Issue: 1 Pages: e0170006-e0170006
  • DOI: 10.1371/journal.pone.0170006
  • NAID: 120005980875
  • Peer Reviewed / Open Access
• [Journal Article] Severe mitochondrial damage associated with low-dose radiation sensitivity in ATM- and NBS1-deficient cells. (2016)
  • Author(s): Shimura T, Kobayashi J, Komatsu K, Kunugita N.
  • Journal Title: Cell Cycle Volume: 15 Issue: 8 Pages: 1099-1107
  • DOI: 10.1080/15384101.2016.1156276
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Induction of Excess Centrosomes in Neural Progenitor Cells during the Development of Radiation-Induced Microcephaly. (2016)
  • Author(s): Shimada M, Matsuzaki F, Kato A, Kobayashi J, Matsumoto T, Komatsu K.
  • Journal Title: PLoS One Volume: 11 Issue: 7 Pages: e0158236-e0158236
  • DOI: 10.1371/journal.pone.0158236
  • NAID: 120006348291
  • Peer Reviewed / Open Access
• [Journal Article] Increased oxidative stress in AOA3 cells disturbs ATM-dependent DNA damage responses (2015)
  • Author(s): Kobayashi J, et al.
  • Journal Title: Mutat Res Genet Toxicol Environ Mutagen Volume: 782 Pages: 42-50
  • DOI: 10.1016/j.mrgentox.2015.03.012
  • NAID: 120005670865
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Chromatin modification and NBS1: their relationship in DNA double-strand break repair (2015)
  • Author(s): Saito Y, Zhou H, Kobayashi J.
  • Journal Title: Genes & Genetic Systems Volume: 90 Issue: 4 Pages: 195-208
  • DOI: 10.1266/ggs.15-00010
  • NAID: 130005118400
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] KDEL receptor 1 regulates T-cell homeostasis via PP1 that is a key phosphatase for ISR. (2015)
  • Author(s): Kamimura, D., K. Katsunuma, Y. Arima, T. Atsumi, J-J Jiang, H. Bando, J. Meng, L. Sabharwal, A. Stofkova, N. Nishikawa, H. Suzuki, H. Ogura, N. Ueda, M. Tsuruoka, M. Harada, J. Kobayashi, T. Hasegawa, H. Yoshida, H. Koseki, I. Miura, S. Wakana, K. Nishida, H. Kitamura, T. Fukada, T. Hirano, and M. Murakami
  • Journal Title: Nat. Commun. Volume: 6 Issue: 1 Pages: 7474-7474
  • DOI: 10.1038/ncomms8474
  • NAID: 120005647268
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Excess processing of oxidative damaged bases hypersensitivity to oxidative stress and low dose rate irradiation. (2015)
  • Author(s): Yoshikawa Y, Kobayashi J, et al.
  • Journal Title: Free Radic Res. Volume: 49 Issue: 10 Pages: 1239-1248
  • DOI: 10.3109/10715762.2015.1061186
  • Peer Reviewed / Open Access
• [Journal Article] BRCA1 and CtIP are both required to recruit dna2 at double-strand breaks in homologous recombination. (2015)
  • Author(s): Hoa NN, Kobayashi J, Omura M, Hirakawa M, Yang SH, Komatsu K, Paull TT, Takeda S, Sasanuma H.
  • Journal Title: PLoS One Volume: 10 Issue: 4 Pages: e0124495-e0124495
  • DOI: 10.1371/journal.pone.0124495
  • NAID: 120005615636
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] nucleolinによるDNA複製ストレス応答の制御 (2017)
  • Author(s): 河村香寿美、Qi Fei、加藤竹雄、松浦伸也、小松賢志、小林純也
  • Organizer: 2017年度生命科学系学会合同年次大会（ConBio2017）
• [Presentation] Regulation of various DNA damage responses through complex formations of NBS1. (2015)
  • Author(s): Kobayashi J
  • Organizer: 16th Ataxia-Telangiectasia Workshop (ATW2015)
  • Place of Presentation: Beijing, China
  • Year and Date: 2015-10-11
  • Int'l Joint Research / Invited
• [Presentation] DNA damage-dependent formation of MRE11/RAD50/NBS1 complex. (2015)
  • Author(s): Kobayashi J
  • Organizer: 15th International Congress of Radiation Research (ICRR2015)
  • Place of Presentation: 京都
  • Year and Date: 2015-05-25
  • Int'l Joint Research