| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
We analyzed transcription-coupled nucleotide excision repair (TC-NER), a subpathway of nucleotide excision repair that rapidly removes transcription-blocking DNA damage, and the following results on the functions of TC-NER factors were obtained. (1) The C-terminal region and SUMOylation of CSB plays critical roles in TC-NER. (2) UVSSA is degraded by proteasome upon dissociation from USP7, resulting in TC - NER deficiency, but when a mutation is introduced at the ubiquitination site, degradation is suppressed and TC - NER becomes normal. (3) Ubiquitination of RNA polymerase II may be involved in the restart of transcription after removal of transcription-blocking DNA damage. These results are important cues to reveal molecular mechanism of TC-NER.
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