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Development of aquatic environment restoration technology using molecular breeding strain of cyanotoxin-degrading bacteria

Research Project

Project/Area Number 15H02840
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental engineering and reduction of environmental burden
Research InstitutionUniversity of Tsukuba (2016-2017)
Toyo University (2015)

Principal Investigator

SHIMIZU Kazuya  筑波大学, 生命環境系, 准教授 (10581613)

Co-Investigator(Kenkyū-buntansha) 杉浦 則夫  筑波大学, 生命環境系(名誉教授), 名誉教授 (10302374)
間世田 英明  国立研究開発法人産業技術総合研究所, 生命工学領域, 上級主任研究員 (10372343)
岡野 邦宏  秋田県立大学, 生物資源科学部, 助教 (30455927)
内海 真生  筑波大学, 生命環境系, 准教授 (60323250)
原 啓文  長岡技術科学大学, 学内共同利用施設等, 客員研究員 (80511071)
Research Collaborator Niwooti Whangchai  
IWAMOTO Iwamoto  
Zuriati Zakaria  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords汚濁質除去 / 生物膜法 / 淡水資源 / 藍藻類産生有毒物質 / 浄水処理技術 / 汚染質除去技術 / 汚染質除去 / 浄水処置技術
Outline of Final Research Achievements

In this study, we completed the whole genome sequence of microcystin-degrading bacteria, and then made and used various molecular breeding strains to identify microcystin degradation pathway and intracellular localization of microcystin-degrading enzymes. We got the results that MlrA degrade most microcystin analogues. In addition, we have constructed an aquatic environment restoration technology by useful bacteria, protozoa, and micro-animal that can be utilized at the site which is occurred cyanobacterial bloom.
This research has shown the possibility of innovating water treatment technology utilizing molecular breeding strains and also provides the aquatic environment restoration and water treatment technology that can be used on the actual site.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (15 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (5 results) Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results) Remarks (2 results)

  • [Int'l Joint Research] Maejo University(タイ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] MJIIT(マレーシア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Maejo University(タイ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Maejo University(タイ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] University of Technology Malaysia/MJIIT(マレーシア)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Removal of Microcystis aeruginosa cells and microcystin-LR using ceramic carrier in a continuous flow bioreactor2016

    • Author(s)
      Kazuya Shimizu, Motoo Utsumi, Kunihiro Okano, Tomoaki Itayama, Norio Iwami, Hideaki Maseda, Hiroyuki Kinohira, Jieming Li, Yuhei Inamori, Zhenya Zhang, and Norio Sugiura
    • Journal Title

      Japanese Journal of Water Treatment Biology

      Volume: 52 Pages: 35-43

    • NAID

      130006471098

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Whole-Genome Sequence of the Microcystin-Degrading Bacterium Sphingopyxis sp. C-12015

    • Author(s)
      Kunihiro Okano, Kazuya Shimizu, Hideaki Maseda, Yukio Kawauchi, Motoo Utsumi, Tomoaki Itayama, Zhenya Zhang, Norio Sugiura
    • Journal Title

      Genome Announcements

      Volume: 3 Issue: 4

    • DOI

      10.1128/genomea.00838-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 藍藻毒が藍藻類捕食者に及ぼす影響とその適応機構の解明2018

    • Author(s)
      柳谷将、岩澤俊平、板山朋聡、 雷中方、 内海真生、張振亜、角野立夫、清水和哉
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Microcystin分解酵素遺伝子mlrAの特性―遺伝子マーカーと転写制御機構―2017

    • Author(s)
      古田土実和、新井美智子、柳谷将、岡野邦宏、 内海真生、張振亜、杉浦則夫、間世田英明、清水和哉
    • Organizer
      第51回日本水環境学会年会
    • Place of Presentation
      熊本大学(熊本県・熊本市)
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Microcystin初発分解酵素MlrAのmicrocystin分解特性2016

    • Author(s)
      古田土実和、新井美智子、黒古成美、岡野邦宏、内海真生、張振亜、杉浦則夫、間世田英明、清水和哉
    • Organizer
      日本水処理生物学会第53回大会
    • Place of Presentation
      千葉工業大学(千葉県・習志野市)
    • Year and Date
      2016-11-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] Removal of cyanobacteria and cyanotoxin using micro-animal and co-existing bacteria in a bioreactor2016

    • Author(s)
      Kazuya Shimizu, Kunihiro Okano, Hiroyuki Kinohira, Hideaki Maseda, Norio Iwami, Tomoaki Itayama, Zhenya Zhang, Motoo Utsumi, Norio Sugiura
    • Organizer
      16th International Symposium on Microbial Ecology (ISME16)
    • Place of Presentation
      Montreal Canada
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] MlrA, the essential enzyme for microcystins biodegradation2016

    • Author(s)
      Miwa Kodato, Michiko Arai, Narumi Kurogo, Kunihiro Okano, Zhenya Zhang, Motoo Utsumi, Norio Sugiura, Hideaki Maseda, Kazuya Shimizu
    • Organizer
      16th International Symposium on Microbial Ecology (ISME16)
    • Place of Presentation
      Montreal Canada
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microcystin分解菌によるmicrocystin分解特性2016

    • Author(s)
      黒古成美、岡野邦宏、内海真生、杉浦則夫、間世田英明、清水和哉
    • Organizer
      第50回日本水環境学会年会
    • Place of Presentation
      アスティ徳島、徳島県徳島市
    • Year and Date
      2016-03-16
    • Related Report
      2015 Annual Research Report
  • [Remarks] 研究紹介

    • URL

      https://researchmap.jp/kazuya-urso/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 東洋大学分子生態学研究室

    • URL

      http://www.mizu-env.jp

    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2024-12-25  

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