Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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Outline of Final Research Achievements |
The aim of this study was to clarify the mechanism of aquatic plants to accumulate specific microorganisms in the rhizosphere from the characteristics of microbial communities formed in the rhizosphere and gene expression analysis of aquatic plants coexisting with rhizosphere microorganisms. In the rhizosphere of Spirodela polyrrhiza and Lemna minor, two microorganism groups which show different accumulating behavior were confirmed. The one group showed quick, that is within 3 days, accumulation to the rhizosphere. Another group showed slow accumulation but steadily over time. As the characteristic of the microorganism group selectively accumulating in the rhizosphere, the presence of motility seemed one of important factors. Although the gene expression analysis of S. polyrrhiza coexisting with rhizobacteria was failed because of the low quality of mRNA extracted from S. polyrrhiza, we could develop a series of experimental methods including improvement points.
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