Oxygen dynamic imaging of cell

• Project/Area Number: 15H03003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: KAMACHI Toshiaki 東京工業大学, 生命理工学院, 教授 (30272694)
• Co-Investigator(Kenkyū-buntansha): 伊藤 栄紘 東京工業大学, 生命理工学院, 特任助教 (70707918) ; 田畠 健治 第一薬科大学, 薬学部, 准教授 (80312263)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: 細胞イメージング / りん光プローブ / 活性酸素 / 酸素イメージング / 細胞 / 脂肪細胞 / 低酸素 / がん細胞 / Hypoxia / 1細胞 / 酸素ダイナミクス

Outline of Final Research Achievements

Single cell analysis of lipid metabolism was investigate with phosphorescence lifetime confocal microscopy. 3T3-L1 cell was differentiated to adipocyte so that the oxygen consumption rate of adipocyte was higher than that of preadipocyte due to increase in the number of mitochondria in the cell. Lipid droplet degradation measurment was carried out with oxygen concentration measurement imaging to clarify the importance of oxygen,
Effect of low oxygen stress on the oxygen consumption within cell was evaluated. Antioxidant enzyme systems were induced with the lowering oxygen concentration. Oxygen consumption rate was reduced by the acurate hipoxic stress.

Academic Significance and Societal Importance of the Research Achievements

本申請研究では、既設の酸素濃度イメージング共焦点光学顕微鏡を用いて、細胞内酸素濃度のダイナミクスを明らかにすることを目的とする。一細胞内の酸素濃度のダイナミックスを測定することにより、これまで未開拓であった細胞内酸素濃度イメージングによる様々な細胞機能・生命現象の追跡を行った。本申請研究では、特に、外部刺激による酸素ダイナミクス測定を通して、様々な疾病、癌の難治療性などの現象の解明につながる研究が遂行できた。

Research Products

• [Journal Article] Methane Hydroxylation with Water as an Electron Donor under Light Irradiation in the Presence of Reconstituted Membranes Containing both Photosystem?II and a Methane Monooxygenase (2018)
  • Author(s): Ito Hidehiro、Kondo Ryuichi、Yoshimori Kosei、Kamachi Toshiaki
  • Journal Title: ChemBioChem Volume: 19 Issue: 20 Pages: 2152-2155
  • DOI: 10.1002/cbic.201800324
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Specific interaction between redox phospholipid polymers and plastoquinone in photosynthetic electron transport chain (2017)
  • Author(s): Kenya Tanaka, Masahiro Kaneko, Masahito Ishikawa, Souichiro Kato, Hidehiro Ito, Toshiaki Kamachi, Kazuhide Kamiya, Shuji Nakanishi
  • Journal Title: ChemPhysChem Volume: 18 Issue: 8 Pages: 878-881
  • DOI: 10.1002/cphc.201700065
  • Peer Reviewed
• [Journal Article] Bactericidal Action of Photo-Irradiated Aqueous Extracts from the Residue of Crushed Grapes from Winemaking (2016)
  • Author(s): Tsukada, M., Sheng, H., Tada, M., Mokudai, T., Oizumi, S., Kamachi, T. & Niwano, Y.
  • Journal Title: Biocontrol Science Volume: 21 Issue: 2 Pages: 113-121
  • DOI: 10.4265/bio.21.113
  • NAID: 130005160454
  • ISSN: 1342-4815, 1884-0205
  • Peer Reviewed / Open Access
• [Journal Article] Microbicidal action of photoirradiated aqueous extracts from wine lees (2016)
  • Author(s): Tsukada, M., Sheng, H., Kamachi, T. & Niwano, Y.
  • Journal Title: Journal of Food Science and Technology Volume: 1 Issue: 7 Pages: 1-8
  • DOI: 10.1007/s13197-016-2273-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prooxidative Potential of Photo-Irradiated Aqueous Extracts of Grape Pomace, a Recyclable Resource from Winemaking Process (2016)
  • Author(s): Tsukada, M., Nakashima, T., Kamachi, T. & Niwano
  • Journal Title: PLoS ONE Volume: 11 Issue: 6 Pages: e0158197-e0158197
  • DOI: 10.1371/journal.pone.0158197
  • Peer Reviewed / Open Access
• [Journal Article] Non-catalyzed one-step synthesis of ammonia from atmospheric air and water (2016)
  • Author(s): Haruyama, T., Namise, T., Shimoshimizu, N., Uemura, S., Takatsuji, Y., Hino, M., Yamasaki, R., Kamachi, T. & Kohno, M
  • Journal Title: Green Chemistry Volume: 18 Issue: 16 Pages: 4536-4541
  • DOI: 10.1039/c6gc01560c
  • Peer Reviewed / Open Access
• [Journal Article] High resolution imaging of intracellular oxygen concentration by phosphorescence lifetime (2015)
  • Author(s): Kurokawa H, Ito H, Inoue M, Tabata K, Sato Y, Yamagata K, Kizaka-Kondoh S, Kadonosono T, Yano S, Kamachi T.
  • Journal Title: Sci Rep. Volume: 5 Issue: 1 Pages: 10657-10657
  • DOI: 10.1038/srep10657
  • Peer Reviewed / Open Access
• [Presentation] 光化学系II再構成メタンモノオキシゲナーゼ膜画分を用いたメタン酸化反応 (2018)
  • Author(s): 近藤 龍一・森 史也・伊藤 栄紘・蒲池 利章
  • Organizer: 第70回日本生物工学会大会
• [Presentation] 細胞内酸素イメージングのための白金ポルフィリン修飾メソポーラスシリカナノ粒子の調製 (2018)
  • Author(s): 尾台俊亮・伊藤栄紘・岡本昌樹・蒲池利章
  • Organizer: 第12回バイオ関連化学シンポジウム
• [Presentation] INTRACELLULAR OXYGEN CONCENTRATION IMAGING USING MESOPOROUS SILICA NANOPARTICLES MODIFIED PLATINUM-PORPHYRIN (2018)
  • Author(s): ODAI, Shunsuke; Ito, Hidehiro; Kamachi, Toshiaki
  • Organizer: AsBIC9
  • Int'l Joint Research
• [Presentation] PHOTOINDUCED METHANE HYDROXYLATION USING PHOTOSYSTEM II RECONSTITUTED MEMBRANES CONTAINING PARTICULATE METHANE MONOOXYGENASE (2018)
  • Author(s): Hidehiro ITO, Ryuichi KONDO, Kosei YOSHIMORI, Fumiya MORI, Toshiaki KAMACHI
  • Organizer: AsBIC9
  • Int'l Joint Research
• [Presentation] Hydrogen peroxide imaging using polystyrene beads containing platinum porphyrin (2018)
  • Author(s): Hidehiro Ito, Shunsuke Odai, Toshiaki Kamachi
  • Organizer: 日本化学会第99春期年会
• [Presentation] T3-L1細胞における脂肪滴と細胞内酸素濃度の同時イメージング (2018)
  • Author(s): 前田 和真・尾台 俊亮・伊藤栄紘・蒲池 利章
  • Organizer: 日本化学会第98春期年会
• [Presentation] 白金ポルフィリン修飾メソポーラスシリカナノ粒子の調製と 細胞内酸素濃度イメージングへの利用 (2018)
  • Author(s): 尾台俊亮・伊藤栄紘・岡本昌樹・蒲池利章
  • Organizer: 日本化学会第98春期年会
• [Presentation] 細胞内小器官における酸素消費の低酸素応答 (2017)
  • Author(s): 松崎真衣、小林友輝斗、伊藤栄紘、蒲池利章
  • Organizer: 日本化学会第97春期年会
• [Presentation] 細胞周期に依存した細胞内酸素濃度変化 (2017)
  • Author(s): 小林友輝斗、黒川宏美、伊藤栄紘、井上正宏、蒲池利章
  • Organizer: 日本化学会第97春期年会
• [Presentation] Thermosynechococcus elongatus BP-1由来PSIIを利用したメタンモノオキシゲナーゼによる光駆動プロピレン酸化 (2017)
  • Author(s): 近藤龍一、森史也、伊藤栄紘、蒲池利章
  • Organizer: 第16回 微生物研究会
• [Presentation] Measurement of Oxygen Concentration Dynamics Inside a Single Cell by PLIM (2017)
  • Author(s): Toshiaki Kamachi and Hidehiro Ito
  • Organizer: 14th International Symposium on Applied Bioinorganic Chemistry
  • Int'l Joint Research
• [Presentation] PHOSPHORESCENCE LIFETIME MEASUREMENT FOR O2 CONCENTRATION IMAGING INSIDE A SINGLE CELL (2017)
  • Author(s): Toshiaki Kamachi、 Mai Matsuzaki、Hidehiro Ito
  • Organizer: The Ninth JSME-KSME Thermal and Fluids Engineering Conference (TFEC9)
  • Int'l Joint Research
• [Presentation] Oxygen Concentration Imaging Inside a Single Cell by PLIM (2016)
  • Author(s): Toshiaki Kamachi, Hiromi Kurokawa, Hidehiro Ito
  • Organizer: 8th Asian Biological Inorganic Chemistry Conference
  • Place of Presentation: New Zealand
  • Year and Date: 2016-12-04
  • Int'l Joint Research
• [Presentation] リン光寿命イメージング測定を用いた外部刺激による細胞内酸素濃度変化観察 (2016)
  • Author(s): 伊藤栄紘、黒川宏美、小林友輝斗、松崎真衣、前田和真、佐藤叔史、井上正宏、蒲池利章
  • Organizer: 第10回バイオ関連化学シンポジウム
  • Place of Presentation: 金沢
  • Year and Date: 2016-09-07
• [Presentation] ワイン製造残渣水抽出物への光照射による殺菌活性と活性酸素生成 (2016)
  • Author(s): 多田美香 ，塚田愛，蒲池利章，庭野吉己
  • Organizer: 第69回日本酸化ストレス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2016-08-30
• [Presentation] 白金ポルフィリンデンドリマーの合成と細胞内酸素濃度イメージング (2016)
  • Author(s): 尾台俊亮、松崎真衣、八馬彬、伊藤栄紘、蒲池利章
  • Organizer: 日本化学会第96春期年会
  • Place of Presentation: 同志社
  • Year and Date: 2016-03-23
• [Presentation] Oxygen Concentration Imaging Inside a Single Cell by PLIM (2016)
  • Author(s): Toshiaki Kamachi, Hiromi Kurokawa, Hidehiro Ito
  • Organizer: 12th Advanced Imaging Methods Workshop
  • Place of Presentation: Bｅｒｋｅｌｅｙ， USA
  • Year and Date: 2016-02-04
  • Int'l Joint Research / Invited
• [Presentation] Phosphorescence lifetime measurement for oxygen concentration imaging inside a single cel (2015)
  • Author(s): Kamachi, Toshiaki
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2015
  • Place of Presentation: Ｈａｗａｉｉ， ＵＳＡ
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] リン光寿命イメージングによる1細胞内酸素濃度イメージング (2015)
  • Author(s): 蒲池利章
  • Organizer: Biochemical and Molecular Biology2015
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] Phosphorescence lifetime measurement for oxygen concentration imaging of cell (2015)
  • Author(s): Kamachi, Toshiaki
  • Organizer: 5th Japanese-German Joint Seminar
  • Place of Presentation: つくば
  • Year and Date: 2015-09-23
  • Int'l Joint Research / Invited
• [Presentation] リン光寿命測定を利用した酸素濃度イメージングによる細胞内低酸素領域の解明 (2015)
  • Author(s): 伊藤栄紘、黒川宏美、小林友輝斗、田畠健治、矢野重信、井上正宏、蒲池 利章
  • Organizer: 第9回バイオ関連化学シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 2015-09-10