| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Outline of Final Research Achievements |
We aimed to establish a novel "megakaryocyte maturation / platelet release system" that enables to generate bona fide platelets with the same function as in vivo with high efficiency. Based on the live bone marrow observation of mouse megakaryocytes protruding their cytoplasm into sinusoid blood vessels and producing platelets under blood flow stimulation, we analyzed flow vectors in those images. The results indicated that turbulence in the blood flow are present adjacent to the cell wall of megakaryocytes from which platelet production event is continuously observed. We then prepared a new model culture tank that demonstrated new mechanisms of how turbulence regulates remodeling of megakaryocytes and promotes platelet production. Using this model, we found conditions that enabled to produce 100 billion platelets by 8L-scale tank. These findings led to creating a new research area of a turbulent flow-controlled thrombopoiesis mechanism.
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