Development of innovative detection system for bacteria and cells using biostructure-imprinted polymer film
| Project/Area Number |
15H03010
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
TOKONAMI Shiho 大阪府立大学, 工学(系)研究科(研究院), 准教授 (60535491)
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| Co-Investigator(Kenkyū-buntansha) |
飯田 琢也 大阪府立大学, 理学(系)研究科(研究院), 准教授 (10405350)
中瀬 生彦 大阪府立大学, 理学(系)研究科(研究院), 准教授 (40432322)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | バイオセンサ / 分子認識 / 細菌 / 細胞 / 機能性高分子 / 分析化学 |
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| Outline of Final Research Achievements |
Our aim in this study was the creation of an innovative detection system to realize the rapid and easy specific detection of small amount of pathogenic bacteria and cancer cells in a few minutes by developing a "biostructure-imprinted polymer film", where the surface structure of the detection target is three-dimensionally memorized.
We elucidated the mechanism of selective trapping of bacteria and cells into the biostructure-imprinted polymer films based on both experimental and theoretical approaches. Based on this mechanism, by producing cell-imprinted films of leukemia cells and bacteria-imprinted films of coliform group, we have succeeded in the specific detection of them and clarified that highly sensitive detection was possible even in the case of low concentration around 1000 cells / mL.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の学術的意義は、細菌・細胞表面の糖鎖などの表面化学構造がポリマー膜に転写されて選択的な捕捉ができることを物理化学的アプローチで解明できたことであり、化学・生命科学・臨床医学など多分野に渡るインパクトがあると考えている。さらに、病原性細菌・腫瘍細胞の迅速かつ高感度な検出システムの基礎構築に社会的意義があり、発展研究が推進されることで医療分野・食品業界への多大な波及効果が今後期待される。
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Report
(4 results)
Research Products
(144 results)
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[Presentation] ハニカム電極を用いた微生物燃料電池2017
Author(s)
栗田慎也, 西村勇姿, 山本靖之, Olaf Karthaus, 飯田琢也, 床波志保
Organizer
第64回応用物理学会春季学術講演会
Place of Presentation
パシフィコ横浜(神奈川県横浜市)
Year and Date
2017-03-14
Related Report
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[Presentation] Densely Fixed Bacteria Using Photoinduced Convection2016
Author(s)
S. Kurita, Y. Nishimura, Y. Yamamoto, O. Karthaus, T. Iida, S. Tokonami
Organizer
OPU-KIST Joint Symposium on Next Generation Photochemistry and Photophysics: From Materials to Applications
Place of Presentation
KIST（Seoul, South Korea）
Year and Date
2016-09-25
Related Report
Int'l Joint Research
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[Presentation] 分析空間創成に基づく迅速検出法の開発2016
Author(s)
床波志保, 椎木弘, 長岡勉, 中瀬生彦, 田村守, 飯田琢也
Organizer
日本分析化学会第65年会
Place of Presentation
北海道大学工学部(北海道札幌市)
Year and Date
2016-09-14
Related Report
Invited
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[Presentation] 光誘起対流を利用した生細菌の高密度捕捉2016
Author(s)
栗田慎也, 沼田紘志, 西村勇姿, 山本靖之, Karthaus Olaf, 飯田琢也, 床波志保
Organizer
日本分析化学会第65年会
Place of Presentation
北海道大学工学部(北海道札幌市)
Year and Date
2016-09-14
Related Report
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[Presentation] Rapid Bacterial Counting Method using Photothermal Effect2016
Author(s)
Y. Yamamoto, E. Shimizu, Y. Nishimura, S. Tokonami, T. Iida
Organizer
The 14th International Conference of Near-Field Optics, Nanophotonics and Related Techniques(NFO-14)
Place of Presentation
Okuara Act City Hotel Hamamatsu（Hamamatsu, Japan）
Year and Date
2016-09-04
Related Report
Int'l Joint Research
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[Presentation] 外場を利用した生細菌の高密度固定2016
Author(s)
栗田慎也, 西村勇姿, 山本靖之, 飯田琢也, Olaf Karthaus, 床波志保
Organizer
第76回分析化学討論会
Place of Presentation
岐阜薬科大学・岐阜大学(岐阜県岐阜市)
Year and Date
2016-05-28
Related Report
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[Presentation] Photothermal assembling of porphyrin dimers2015
Author(s)
Y. Yamamoto, Y. Nishimura, T. Tanaka, H. Yorimitsu, A. Osuka, S. Tokonami, T. Iida
Organizer
The International Chemical Congress of Pacific Basin Societies 2015（Pacifichem2015)
Place of Presentation
The Sheraton Waikiki HotelHonolulu, Hawaii, USA
Year and Date
2015-12-15
Related Report
Int'l Joint Research
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