Fabrication of highly active antigen nanocarriers by integration of immunity-inducing functions and their application to cancer immunotherapy

• Project/Area Number: 15H03024
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Osaka Prefecture University
• Principal Investigator: YUBA Eiji 大阪府立大学, 工学(系)研究科(研究院), 准教授 (80582296)
• Co-Investigator(Kenkyū-buntansha): 宇高 恵子 高知大学, 教育研究部医療学系基礎医学部門, 教授 (40263066)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2015: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
• Keywords: リポソーム / がん免疫療法 / pH応答性高分子 / アジュバント / 免疫抑制解除 / がん抗原ペプチド / がんワクチン / 細胞傷害性Tリンパ球 / 膜融合 / 樹状細胞 / サイトゾルデリバリー / TGF-β / 細胞傷害性T細胞 / ペプチドワクチン / 生体材料 / ナノバイオ / 免疫学 / 細胞質デリバリー / ナノワクチン / 制御性T細胞

Outline of Final Research Achievements

In this study, we developed cancer antigen-loaded liposome-based nanocarrier systems integrated functional molecules for intracellular antigen delivery, activation of immune cells and cancel of immunosuppression. Integration of purposeful functional molecules for cancer immunotherapy to liposomes achieved the induction of antigen-specific immune responses and highly cancer therapeutic effect on tumor-bearing mice models. Therefore, these nanocarrier systems are promising as immunity-inducing systems for cancer immunotherapy.

Research Products

• [Journal Article] Bleomycin-loaded pH-sensitive polymer-lipid-incorporated liposomes for cancer chemotherapy (2018)
  • Author(s): E. Yuba, T. Osaki, M. Ono, S. Park, A. Harada, M. Yamashita, K. Azuma, T. Tsuka, N. Ito, T. Imagawa, Y. Okamoto
  • Journal Title: Polymers Volume: 10 Issue: 1 Pages: 74-74
  • DOI: 10.3390/polym10010074
  • Peer Reviewed / Open Access
• [Journal Article] Bladder cancer-associated cancer-testis antigen-derived long peptides encompassing both CTL and promiscuous HLA class II-restricted Th cell epitopes induced CD4+ T cells expressing converged T-cell receptor genes in vitro (2018)
  • Author(s): Tsuruta Miki、Ueda Shohei、Yew Poh Yin、Fukuda Isao、Yoshimura Sachiko、Kishi Hiroyuki、Hamana Hiroshi、Hirayama Masatoshi、Yatsuda Junji、Irie Atsushi、Senju Satoru、Yuba Eiji、Kamba Tomomi、Eto Masatoshi、Nakayama Hideki、Nishimura Yasuharu
  • Journal Title: OncoImmunology Volume: 7 Issue: 4 Pages: 1415687-1415687
  • DOI: 10.1080/2162402x.2017.1415687
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of pH-sensitive fusogenic polymer-modified liposomes co-loaded with antigen and α-galactosylceramide as an anti-tumor vaccine (2018)
  • Author(s): OKAZAKI Seiji、IWASAKI Tadashi、YUBA Eiji、WATARAI Shinobu
  • Journal Title: Journal of Veterinary Medical Science Volume: 80 Issue: 2 Pages: 197-204
  • DOI: 10.1292/jvms.17-0491
  • NAID: 130006339618
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Designing immunostimulatory double stranded messenger RNA with maintained translational activity through hybridization with poly A sequences for effective vaccination (2018)
  • Author(s): Uchida Satoshi、Yoshinaga Naoto、Yanagihara Kayoko、Yuba Eiji、Kataoka Kazunori、Itaka Keiji
  • Journal Title: Biomaterials Volume: 150 Pages: 162-170
  • DOI: 10.1016/j.biomaterials.2017.09.033
  • Peer Reviewed
• [Journal Article] DDS応用を指向した刺激応答性リポソームの設計 (2018)
  • Author(s): 弓場 英司、原田 敦史
  • Journal Title: Farumashia Volume: 54 Issue: 1 Pages: 11-15
  • DOI: 10.14894/faruawpsj.54.1_11
  • NAID: 130006301377
  • ISSN: 0014-8601, 2189-7026
  • Peer Reviewed
• [Journal Article] Hyaluronic Acid-Based pH-Sensitive Polymer-Modified Liposomes for Cell-Specific Intracellular Drug Delivery Systems (2017)
  • Author(s): Miyazaki Maiko、Yuba Eiji、Hayashi Hiroshi、Harada Atsushi、Kono Kenji
  • Journal Title: Bioconjugate Chem. Volume: 29 Issue: 1 Pages: 44-55
  • DOI: 10.1021/acs.bioconjchem.7b00551
  • NAID: 120006719650
  • Peer Reviewed
• [Journal Article] Liposome-based immunity-inducing systems for cancer immunotherapy (2017)
  • Author(s): E. Yuba
  • Journal Title: Molecular Immunology Volume: - Pages: 8-12
  • DOI: 10.1016/j.molimm.2017.11.001
  • NAID: 120006719652
  • Peer Reviewed
• [Journal Article] pH-Responsive Micelle-Based Cytoplasmic Delivery System for Induction of Cellular Immunity (2017)
  • Author(s): E. Yuba, N. Sakaguchi, Y. Kanda, M. Miyazaki, K. Koiwai
  • Journal Title: Vaccines Volume: 5 Issue: 4 Pages: 41-41
  • DOI: 10.3390/vaccines5040041
  • Peer Reviewed / Open Access
• [Journal Article] Carboxylated phytosterol derivative-introduced liposomes for skin environment-responsive transdermal drug delivery system (2017)
  • Author(s): N. Yamazaki, S. Yamakawa, T. Sugimoto, Y. Yoshizaki, R. Teranishi, T. Hayashi, A. Kotaka, C. Shinde, T. Kumei, Y. Sumida, T. Shimizu, Y. Ohashi, E. Yuba, A. Harada, K. Kono
  • Journal Title: J. Liposome Res. Volume: - Issue: 4 Pages: 275-284
  • DOI: 10.1080/08982104.2017.1369995
  • NAID: 120006719651
  • Peer Reviewed
• [Journal Article] Development of pH-sensitive Dextran Derivatives with Strong Adjuvant Function and Their Application to Antigen Delivery (2017)
  • Author(s): Yuba Eiji、Uesugi Shinya、Miyazaki Maiko、Kado Yuna、Harada Atsushi、Kono Kenji
  • Journal Title: Membranes Volume: 7 Issue: 3 Pages: 41-41
  • DOI: 10.3390/membranes7030041
  • Peer Reviewed / Open Access
• [Journal Article] pH-sensitive polymer-modified liposome-based immunity-inducing system: Effects of inclusion of cationic lipid and CpG-DNA (2017)
  • Author(s): Yoshizaki Yuta、Yuba Eiji、Sakaguchi Naoki、Koiwai Kazunori、Harada Atsushi、Kono Kenji
  • Journal Title: Biomaterials Volume: 141 Pages: 272-283
  • DOI: 10.1016/j.biomaterials.2017.07.001
  • NAID: 120006719632
  • Peer Reviewed
• [Journal Article] Potentiation of cancer immunity-inducing effect by pH-sensitive polysaccharide-modified liposomes with combination of TGF-β type I receptor inhibitor-embedded liposomes (2017)
  • Author(s): Yuba Eiji
  • Journal Title: Med. Res. Arch. Volume: 5 Issue: 5
  • DOI: 10.18103/mra.v5i5.1243
  • Peer Reviewed / Open Access
• [Journal Article] Preparation of dual-stimuli-responsive liposomes using methacrylate-based copolymers with pH and temperature sensitivities for precisely controlled release (2017)
  • Author(s): T．Sugimoto, N. Yamazaki, T. Hayashi, E. Yuba, A. Harada, A. Kotaka, C. Shinde, T. Kumei, Y. Sumida, M. Fukushima, Y. Munekata, K. Maruyama, K. Kono
  • Journal Title: Colloids Surf. B: Biointerfaces Volume: 155 Pages: 449-458
  • DOI: 10.1016/j.colsurfb.2017.04.043
  • NAID: 120006719633
  • Peer Reviewed
• [Journal Article] Therapy with transcutaneous administration of imiquimod combined with oral administration of sorafenib suppresses renal cell carcinoma growing in an orthotopic mouse model (2017)
  • Author(s): Karashima Takashi、Udaka Keiko、Niimura Mayumi、Suzuki Katsuhide、Osakabe Hiroto、Shimamoto Tsutomu、Fukata Satoshi、Inoue Keiji、Kuroda Naoto、Seiki Motoharu、Shuin Taro
  • Journal Title: Oncology Letters Volume: 14 Issue: 1 Pages: 1162-1166
  • DOI: 10.3892/ol.2017.6235
  • Peer Reviewed / Open Access
• [Journal Article] Dual-stimuli responsive liposomes using pH- and temperature-sensitive polymers for controlled transdermal delivery (2017)
  • Author(s): N. Yamazaki, T. Sugimoto, M. Fukushima, R. Teranishi, A. Kotaka, C. Shinde, T. Kumei, Y. Sumida, Y. Munekata, K. Maruyama, E. Yuba, A. Harada, K. Kono
  • Journal Title: Polymer Chemistry Volume: 8 Issue: 9 Pages: 1507-1518
  • DOI: 10.1039/c6py01754a
  • NAID: 120006719634
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Bioactive polysaccharide-based pH-sensitive polymers for cytoplasmic delivery of antigen and activation of antigen-specific immunity (2017)
  • Author(s): E. Yuba, A. Yamaguchi, Y. Yoshizaki, A. Harada, K. Kono
  • Journal Title: Biomaterials Volume: 120 Pages: 32-45
  • DOI: 10.1016/j.biomaterials.2016.12.021
  • NAID: 120006719631
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Induction of antibody response in the oral cavity of dogs following intraocular (eye drop) immunization with <i>Porphyromonas gingivalis</i> cell lysate incorporated in pH-sensitive fusogenic polymer-modified liposomes (2017)
  • Author(s): Y. Shimizu, T. Iwasaki, T. Tajima, E. Yuba, K. Kono, S. Watarai
  • Journal Title: Journal of Veterinary Medical Science Volume: 79 Issue: 2 Pages: 290-298
  • DOI: 10.1292/jvms.16-0338
  • NAID: 130005332239
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 免疫機構によるがん細胞攻撃の仕組み (2017)
  • Author(s): 宇高恵子
  • Journal Title: 日本臨床 Volume: 75 Pages: 170-173
• [Journal Article] Improvement of Peptide-Based Tumor Immunotherapy Using pH-Sensitive Fusogenic Polymer-Modified Liposomes. (2016)
  • Author(s): Yoshizaki Y, Yuba E, Komatsu T, Udaka K, Harada A, Kono K
  • Journal Title: Molecules Volume: 21 Issue: 10 Pages: 1284-1284
  • DOI: 10.3390/molecules21101284
  • Peer Reviewed / Open Access
• [Journal Article] 免疫治療のためのDDS最前線 (2016)
  • Author(s): 弓場英司，原田敦史, 河野健司
  • Journal Title: 高分子 Volume: 65 Pages: 435-440
• [Journal Article] Identification of glypican-3-derived long peptides activating both CD8 and CD4 T cells; prolonged overall survival in cancer patients with Th cell response (2016)
  • Author(s): Sayem, M. A. Tomita, Y. Yuno, A. Hirayama, M. Irie, A. Tsukamoto, H. Senju, S. Yuba, E. Yoshikawa, T. Kono, K. Nakatsura, T. Nishimura, Y.
  • Journal Title: Oncoimmunology Volume: 5 Issue: 1 Pages: e1062209-e1062209
  • DOI: 10.1080/2162402x.2015.1062209
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An oncofetal antigen, IMP-3-derived long peptides induce immune responses of both helper T cells and CTLs (2016)
  • Author(s): M. Hirayama, Y. Tomita, A. Yuno, H. Tsukamoto, S. Sejju, Y. Imamura, M. A. Sayem, A. Irie, Y. Yoshitake, D. Fukuma, M. Shinohara, A. Hamada, H. Jono, E. Yuba, K. Kono, K. Yoshida, T. Tsunoda, H. Nakayama, Y. Nishimura
  • Journal Title: OncoImmunology Volume: 5 Issue: 6 Pages: e1123368-e1123368
  • DOI: 10.1080/2162402x.2015.1123368
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Design of pH-sensitive polymer-modified liposomes for antigen delivery and their application in cancer immunotherapy (2016)
  • Author(s): E. Yuba
  • Journal Title: Polymer Journal Volume: 48 Issue: 7 Pages: 761-771
  • DOI: 10.1038/pj.2016.31
  • NAID: 120006719635
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] PAMAM dendron lipid assemblies that undergo structural transition in response to weakly acidic pH and their cytoplasmic delivery capability (2015)
  • Author(s): T. Doura, M. Yamada, R. Teranishi, Y. Yamamoto, T. Sugimoto, E. Yuba, A. Harada, K. Kono
  • Journal Title: Langmuir Volume: 印刷中 Issue: 18 Pages: 5105-5114
  • DOI: 10.1021/acs.langmuir.5b00183
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] pH-Sensitive polymer-liposome-based antigen delivery systems potentiated with interferon-γ gene lipoplex for efficient cancer immunotherapy (2015)
  • Author(s): E. Yuba, Y. Kanda, Y. Yoshizaki, R. Teranishi, A. Harada, K. Sugiura, T. Izawa, J. Yamate, N. Sakaguchi, K. Koiwai, K. Kono,
  • Journal Title: Biomaterials Volume: 67 Pages: 214-224
  • DOI: 10.1016/j.biomaterials.2015.07.031
  • NAID: 120007159642
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Multifunctional liposomes having target specificity, temperature-triggered release, and near-infrared fluorescence imaging for tumor-specific chemotherapy (2015)
  • Author(s): K. Kono, M. Takashima, E. Yuba, A. Harada, Y. Hiramatsu, H. Kitagawa, T. Otani, K. Maruyama, S. Aoshima
  • Journal Title: J. Control. Release Volume: 216 Pages: 69
  • DOI: 10.1016/j.jconrel.2015.08.005
  • URL: https://pure.teikyo.jp/en/publications/ddef4ff1-dd22-4dd6-8d0b-6921ae82f778
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] pH-Responsive polysaccharide-modified liposomes for antigen delivery and induction of immune responses. (2018)
  • Author(s): E. Yuba, A. Yamaguchi, S. Uesugi, A. Harada, K. Kono
  • Organizer: 255th ACS National Meeting & Expositions
  • Int'l Joint Research
• [Presentation] Mannose residue-introduced pH-sensitive curdlan-modified liposome for antigen presenting cell-specific antigen delivery system (2018)
  • Author(s): Y. Fukaya, E. Yuba, A. Harada, K. Kono
  • Organizer: 255th ACS National Meeting & Expositions
  • Int'l Joint Research
• [Presentation] Development of hyaluronic acid derivative-modified liposomes as dendritic cell-specific antigen delivery carriers (2018)
  • Author(s): M. Miyazaki, E. Yuba, H. Hayashi, A. Harada, K. Kono
  • Organizer: 255th ACS National Meeting & Expositions
  • Int'l Joint Research
• [Presentation] pH-sensitive polysaccharides-based immunity-inducing systems (2017)
  • Author(s): E. Yuba, A. Yamaguchi, A. Harada, K. Kono
  • Organizer: 第66回高分子学会年次大会
• [Presentation] A phase I study of novel multi-HLA binding peptides and a new combination of immune adjutants against solid tumors (2017)
  • Author(s): H. Matsui, S. Hazama, K. Tamada, K. Udaka, Y. Koki, T. Uematsu, H. Arima, H. Furukawa, T. Miyakawa, S. Doi, M. Nakajima, M. Kitahara, S. Kanekiyo, Y. Tokumitsu, M. Iida, Y. Tokuhisa, K. Sakamoto, N. Suzuki, S. Takeda, S. Yamamoto, S. Yoshino, T. Ueno, H. Nagano
  • Organizer: American Society of Clinical Oncology (ASCO) 2017 Annual Meeting
  • Int'l Joint Research
• [Presentation] Novel WT1 peptide vaccination for newly diagnosed malignant gliomas (2017)
  • Author(s): Y. Kawasaki, K. Udaka, T. Ueba
  • Organizer: The 36th Sapporo International cancer Symposium
  • Int'l Joint Research
• [Presentation] pH応答性多糖修飾リポソームの抗原ペプチドデリバリキャリアとしての機能 (2017)
  • Author(s): 弓場 英司，能﨑優太，原田 敦史，宇高恵子，西村泰治，河野 健司
  • Organizer: 第33回日本DDS学会学術集会
• [Presentation] マンノースを末端基とするデンドロン脂質の合成とその抗原キャリアの作製 (2017)
  • Author(s): 斉藤良典, 弓場英司, 原田敦史, 河野健司
  • Organizer: 第63回高分子研究発表会(神戸)
• [Presentation] マンノース残基導入pH応答性カードランを修飾したリポソームの抗原デリバリー機能 (2017)
  • Author(s): 深谷佳樹, 弓場英司, 原田敦史, 河野健司
  • Organizer: 第63回高分子研究発表会(神戸)
• [Presentation] Design of functional dendrimer-gold nanorod hybrids for biomedical applications (2017)
  • Author(s): E. Yuba, K. Kono
  • Organizer: IUPAC 17th International Symposium on MacroMolecular Complexes
  • Int'l Joint Research / Invited
• [Presentation] 免疫を活性化して、ワクチンを細胞内に運ぶ。カルボキシ化多糖の力 (2017)
  • Author(s): 弓場 英司
  • Organizer: イノベーション・ジャパン2017
• [Presentation] 抗原送達・免疫誘導のためのpH応答性多糖修飾リポソームの設計 (2017)
  • Author(s): 弓場 英司，山口彩加，上杉慎也，原田 敦史，河野 健司
  • Organizer: 第66回高分子討論会
• [Presentation] pH-Responsive polysaccharide-modified liposomes for intracellular drug delivery (2017)
  • Author(s): E. Yuba
  • Organizer: 13th IUPAC International Conference NMS-XIII
  • Int'l Joint Research / Invited
• [Presentation] 腫瘍に対するＴ細胞戦略 (2017)
  • Author(s): 宇高 恵子
  • Organizer: 第65回ＮＰＯ法人日本口腔科学会中国・四国地方部会
• [Presentation] Construction of a TAP deficient HLA class I-binding assay system with the use of CRISPR/Cas9 system (2017)
  • Author(s): T. Shimizu, T. Komatsu, K.Udaka
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] Extravasation of CTLs occurs by recognizing the MHC-peptide complexes cross-presented by vascular endothelial cells (2017)
  • Author(s): T. Komatsu, R. Iwade, M. Kijima, T. Sato, K. Udaka
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] Application with nano-sized cyanoacryl particles ameliorates experimental dermatitis through the bacteriocidal activity (2017)
  • Author(s): M. Kasai, S. Sano, K. Udaka
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] Topical application of nano-sized, bactericidal polymer particles ameliorates hapten-induced dermatitis (2017)
  • Author(s): K. Udaka, M. Kasai, A. Kawaguchi, R. Kamijima, S. Matsuzaki, K. Suzuki, M. Yamamoto, S. Sano, S. Shirotake
  • Organizer: 日本研究皮膚科学会第42回年次学術大会・総会
• [Presentation] pH応答性カードランとカチオン性脂質をハイブリッド化した高活性リポソームアジュバントの開発 (2017)
  • Author(s): 門柚奈, 弓場英司, 原田敦史, 河野健司
  • Organizer: 第６回バイオ・メディカル・フォーラム
  • Place of Presentation: あべのメディックス(大阪府大阪市)
• [Presentation] 免疫を活性化する・抗原をはこぶ機能性多糖を利用した免疫誘導システム (2017)
  • Author(s): 弓場英司
  • Organizer: メディカルジャパン2017
  • Place of Presentation: インテックス大阪(大阪府大阪市)
• [Presentation] Hyaluronic acid derivative-modified liposomes as intracellular drug delivery system to CD44-expressing cells. (2016)
  • Author(s): M. Miyazaki, E. Yuba, H. Hayashi, A. Harada, K. Kono
  • Organizer: The 11th SPSJ International Polymer Conference
  • Place of Presentation: 福岡国際会議場(福岡県福岡市）
  • Year and Date: 2016-12-13
  • Int'l Joint Research
• [Presentation] Topical treatment with nano-sized particles of cyanoacryl polymer ameliorates experimental dermatitis through backteriocidal effect. (2016)
  • Author(s): Yamamoto M, Kasai M, Kawaguchi A, Kamijima R, Suzuki K, Shirotake S, Sano S, Udaka K
  • Organizer: 日本研究皮膚科学会第４１回年次学術大会・総会
  • Place of Presentation: 仙台国際センター(宮城県仙台市)
  • Year and Date: 2016-12-09
• [Presentation] How do antigen specific DC4 and CD8 T cells contribute to the development of anexperimental contact dermatitis? (2016)
  • Author(s): Okunaga I, Shimizu T, Udaka K
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県宜野湾市)
  • Year and Date: 2016-12-05
• [Presentation] Cross-presentation of tumor antigen by endothelial cells promotes infiltration of tumor specific CTLs (2016)
  • Author(s): Komatsu T, Iwade R, Sato T, Udaka K
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県宜野湾市)
  • Year and Date: 2016-12-05
• [Presentation] Development of effective immune-inducing system using pH-sensitive polysaccharide-modified liposomes and TGF-β signal inhibitor-embedded liposomes (2016)
  • Author(s): E. Yuba, S. Uesugi, A. Harada, K. Kono
  • Organizer: 3rd International Conference on Biomaterials Science in Tokyo (ICBS2016)
  • Place of Presentation: 伊藤謝恩ホール(東京都文京区)
  • Year and Date: 2016-11-28
  • Int'l Joint Research
• [Presentation] pH-Sensitive polymer-modified liposome-based immunity inducing system: the effect of inclusion of cationic lipid and CpG-DNA (2016)
  • Author(s): Y. Yoshizaki, E. Yuba, N. Sakaguchi, K. Koiwai, A. Harada, K. Kono
  • Organizer: 3rd International Conference on Biomaterials Science in Tokyo (ICBS2016)
  • Place of Presentation: 伊藤謝恩ホール(東京都文京区)
  • Year and Date: 2016-11-28
  • Int'l Joint Research
• [Presentation] Hyaluronic acid derivative-modified liposomes for a target-specific intracellular drug delivery system (2016)
  • Author(s): M. Miyazaki, E. Yuba, H. Hayashi, A. Harada, K. Kono
  • Organizer: 3rd International Conference on Biomaterials Science in Tokyo (ICBS2016)
  • Place of Presentation: 伊藤謝恩ホール(東京都文京区)
  • Year and Date: 2016-11-28
  • Int'l Joint Research
• [Presentation] カードラン誘導体修飾リポソームの免疫誘導活性に及ぼすカチオン性脂質導入の効果 (2016)
  • Author(s): 門柚奈、弓場英司、坂口奈央樹、小岩井一倫、原田敦史、河野健司
  • Organizer: 日本バイオマテリアル学会シンポジウム2016
  • Place of Presentation: 福岡国際会議場(福岡県福岡市）
  • Year and Date: 2016-11-21
• [Presentation] サイトカイン遺伝子と抗原の同時送達によるpH応答性リポソームワクチンの高活性化 (2016)
  • Author(s): 弓場英司、神田雄平、原田敦史、河野健司
  • Organizer: 日本バイオマテリアル学会シンポジウム2016
  • Place of Presentation: 福岡国際会議場(福岡県福岡市）
  • Year and Date: 2016-11-21
• [Presentation] Preparation of Polysaccharide Derivatives Having pH-Sensitivity and Immune Activation Function (2016)
  • Author(s): E. Yuba, A. Yamaguchi, A. Harada, K. Kono
  • Organizer: Biomaterials International2016
  • Place of Presentation: Howard Beach Resort Kenting(台湾、墾丁)
  • Year and Date: 2016-10-30
  • Int'l Joint Research
• [Presentation] Liposome engineering for efficient cancer immunotherapy (2016)
  • Author(s): E. Yuba, K. Kono
  • Organizer: 12th IUPAC International Conference NMS-XII
  • Place of Presentation: 湖南農業大学(中国、長沙)
  • Year and Date: 2016-10-14
  • Int'l Joint Research / Invited
• [Presentation] Simultaneous delivery system of antigenic protein and cytokine gene using hybrid complexes of pH-sensitive polymer-modified liposome and lipoplex (2016)
  • Author(s): E. Yuba, Y. Kanda, A. Harada, K. Kono
  • Organizer: 4th Symposium on Innovative Polymers for Controlled Delivery
  • Place of Presentation: Worldhotel Grand Dushulake Suzhou(中国、蘇州)
  • Year and Date: 2016-09-23
  • Int'l Joint Research
• [Presentation] カルボキシ基導入多糖修飾リポソームのpH応答性・抗原デリバリー機能に及ぼす側鎖構造の影響 (2016)
  • Author(s): 弓場 英司、上杉慎也、原田 敦史、河野 健司
  • Organizer: 第65回高分子討論会
  • Place of Presentation: 神奈川大学横浜キャンパス(神奈川県横浜市)
  • Year and Date: 2016-09-14
• [Presentation] 弱酸性pHで構造転移するデンドロン脂質集合体の調製とサイトゾルデリバリーシステムへの展開 (2016)
  • Author(s): 弓場 英司、堂浦智裕、山田めぐみ、原田 敦史、河野 健司
  • Organizer: 第65回高分子討論会
  • Place of Presentation: 神奈川大学横浜キャンパス(神奈川県横浜市)
  • Year and Date: 2016-09-14
• [Presentation] 機能性高分子の表面修飾による細胞特異的リポソームDDSの設計 (2016)
  • Author(s): 弓場 英司
  • Organizer: 粉体工学会第52回夏期シンポジウム
  • Place of Presentation: ひょうご共済会館(兵庫県神戸市)
  • Year and Date: 2016-08-08
  • Invited
• [Presentation] CpG-DNAを複合化したpH応答性高分子修飾リポソームの免疫誘導機能 (2016)
  • Author(s): 能崎 優太、弓場 英司、坂口 奈央樹、小岩井 一倫、原田 敦史、河野 健司
  • Organizer: 第32回日本DDS学会学術集会
  • Place of Presentation: グランシップ（静岡県静岡市）
  • Year and Date: 2016-06-30
• [Presentation] pH応答性多糖修飾リポソームとTGF-βI型受容体阻害剤包埋リポソームを併用したがん免疫誘導システムの構築 (2016)
  • Author(s): 弓場 英司、上杉慎也、原田 敦史、河野 健司
  • Organizer: 第32回日本DDS学会学術集会
  • Place of Presentation: グランシップ（静岡県静岡市）
  • Year and Date: 2016-06-30
• [Presentation] pH応答性膜融合ポリマーとがん細胞膜ベシクルの複合化によるがん抗原デリバリーシステムの構築 (2016)
  • Author(s): 浦﨑拓真、弓場 英司、原田 敦史、河野 健司
  • Organizer: 第32回日本DDS学会学術集会
  • Place of Presentation: グランシップ（静岡県静岡市）
  • Year and Date: 2016-06-30
• [Presentation] TGF-β受容体阻害剤包埋リポソームの併用によるpH応答性多糖修飾リポソームのがん免疫誘導機能の増強 (2016)
  • Author(s): 弓場 英司・上杉慎也・原田 敦史・河野 健司
  • Organizer: 第65回高分子学会年次大会
  • Place of Presentation: 国際会議場・展示場(兵庫県神戸市)
  • Year and Date: 2016-05-25
• [Presentation] 細胞特異性と細胞内デリバリー機能をあわせもつヒアルロン酸誘導体修飾リポソームの構築 (2016)
  • Author(s): 宮﨑麻衣子・弓場 英司・原田 敦史・林弘志・河野 健司
  • Organizer: 第65回高分子学会年次大会
  • Place of Presentation: 国際会議場・展示場(兵庫県神戸市)
  • Year and Date: 2016-05-25
• [Presentation] Cell-derived vesicles modified with pH-sensitive polymers for intracellular delivery of autologous antigen (2016)
  • Author(s): E. Yuba, T. Sakano, T. Kato, T. Urasaki, A. Harada, K. Kono
  • Organizer: 10th World Biomaterials Congress
  • Place of Presentation: Palais des congres de Montreal（カナダ、モントリール）
  • Year and Date: 2016-05-17
  • Int'l Joint Research
• [Presentation] Effect of CpG-DNA inclusion in pH-sensitive polymer-liposome vaccines on their immune-inducing activities (2016)
  • Author(s): Y. Yoshizaki, E. Yuba, N. Sakaguchi, K. Koiwai, A. Harada, K. Kono
  • Organizer: 10th World Biomaterials Congress
  • Place of Presentation: Palais des congres de Montreal（カナダ、モントリール）
  • Year and Date: 2016-05-17
  • Int'l Joint Research
• [Presentation] アジュバント機能と抗原デリバリー機能を併せ持つ多糖をベースにした免疫誘導システム (2016)
  • Author(s): 弓場英司、山口彩加、原田敦史、河野健司
  • Organizer: 第9回次世代アジュバント研究会
  • Place of Presentation: 大阪、千里ライフサイエンスセンター
  • Year and Date: 2016-01-19
• [Presentation] カチオン性脂質・Toll様受容体リガンドを導入したpH応答性高分子修飾リポソームのアジュバント効果とがん免疫治療への応用 (2016)
  • Author(s): 能﨑優太、弓場英司、坂口奈央樹、小岩井一倫、原田敦史、河野健司
  • Organizer: 第9回次世代アジュバント研究会
  • Place of Presentation: 大阪、千里ライフサイエンスセンター
  • Year and Date: 2016-01-19
• [Presentation] 血管内皮細胞のクロスプレゼンテーションによるＣＴＬの固形腫瘍への抗原特異的浸潤 (2016)
  • Author(s): 岩出良介、横山水紀、小松利広、木嶋美香、笠井道之、清水健之、佐藤健人、宇高恵子
  • Organizer: 第１５回四国免疫フォーラム
  • Place of Presentation: 高知大学医学部(高知県南国市)
• [Presentation] 血管内皮細胞のＨＬＡクラスＩＩ発現は、Ｔ細胞浸潤及び膵腫瘍に対する傷害活性と関連する (2016)
  • Author(s): 宇高恵子、木岐 淳、西森 功、西原利治、小松利広、西岡千恵、鈴木勝英
  • Organizer: 第８回血液疾患免疫療法学会
  • Place of Presentation: 北海道大学(北海道札幌市）
• [Presentation] がん免疫療法の開発戦略 -抗原提示の視点から- (2016)
  • Author(s): 宇高 恵子
  • Organizer: 日本臨床麻酔学会第３６回大会
  • Place of Presentation: 高知県立県民文化ホール(高知県高知市)
  • Invited
• [Presentation] Bioactive polysaccharide-based pH-sensitive polymers for cytoplasmic delivery of antigen and activation of antigen-specific immunity (2015)
  • Author(s): Eiji Yuba, Ayaka Yamaguchi, Atsushi Harada, Kenji Kono
  • Organizer: PACIFICHEM2015
  • Place of Presentation: ホノルル、ハワイコンベンションセンター
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Multiple adjuvant molecules-incorporated pH-sensitive polymer-modified liposomes for efficient activation and induction of antigen-specific immunity (2015)
  • Author(s): Ｙuta Yoshizaki, Eiji Yuba, Naoki Sakaguchi, Kazunori Koiwai, Atsushi Harada, Kenji Kono
  • Organizer: PACIFICHEM2015
  • Place of Presentation: ホノルル、ハワイコンベンションセンター
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] 免疫細胞の病源体認識機構を模倣したスマート抗原デリバリーシステムの開発 (2015)
  • Author(s): 弓場英司
  • Organizer: 第4回ネイチャーインダストリーアワード
  • Place of Presentation: 大阪、大阪科学技術センター
  • Year and Date: 2015-12-04
• [Presentation] アジュバント機能と抗原運搬機能を併せ持つpH応答性多糖による免疫誘導システムの開発 (2015)
  • Author(s): 弓場英司
  • Organizer: 第35回バイオ技術シーズ公開会
  • Place of Presentation: 大阪、大阪科学技術センター
  • Year and Date: 2015-12-02
• [Presentation] pH応答性リポソームとTGF-β阻害剤包埋リポソームの併用によるがん免疫治療効果の増強 (2015)
  • Author(s): 上杉慎也，神田雄平、弓場英司、原田敦史、河野健司
  • Organizer: 第35回日本バイオマテリアル学会大会
  • Place of Presentation: 東京、タワーホール船堀
  • Year and Date: 2015-11-25
• [Presentation] pH-Sensitive polymer-modified liposomes incorporated with adjuvants for cancer immunotherapy (2015)
  • Author(s): Yuta Yoshizaki, Eiji Yuba, Naoki Sakaguchi, Kazunori Koiwai, Atsushi Harada, Kenji Kono
  • Organizer: The 3rd OPU-TKU international symposium
  • Place of Presentation: 大阪、大阪府立大学学術交流会館
  • Year and Date: 2015-11-19
  • Int'l Joint Research
• [Presentation] HLA class II-binding peptides exhibit more promiscuous binding to allomorphs than the HLA class I-binding peptides (2015)
  • Author(s): Udaka K, Kasajima R, Chi S, Arima N, Komatsu T
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌、札幌コンベンションセンター
  • Year and Date: 2015-11-18
• [Presentation] 細胞膜由来ベシクルへのpH応答性高分子のハイブリッド化による自家がんワクチンの創製 (2015)
  • Author(s): 弓場英司, 坂野貴宣, 加藤拓実, 浦崎拓真, 原田敦史, 河野健司
  • Organizer: 第64回高分子討論会
  • Place of Presentation: 仙台、東北大学
  • Year and Date: 2015-09-15
• [Presentation] pH応答性高分子・カチオン性脂質・CpG-DNAを用いた多機能型リポソームワクチンの設計 (2015)
  • Author(s): 能崎優太、弓場英司、坂口奈央樹、小岩井一倫、原田敦史、河野健司
  • Organizer: 第64回高分子討論会
  • Place of Presentation: 仙台、東北大学
  • Year and Date: 2015-09-15
• [Presentation] 免疫活性化分子を多重集積化したpH応答性高分子修飾リポソームの作製とがん免疫治療への応用 (2015)
  • Author(s): 能崎優太、弓場英司、坂口奈央樹、小岩井一倫、原田敦史、河野健司
  • Organizer: 第44回 医用高分子シンポジウム
  • Place of Presentation: 東京、産業技術総合研究所 臨海副都心センター
  • Year and Date: 2015-07-27
• [Presentation] Simultaneous Delivery System of Antigenic Protein and Cytokine Gene Based on pH-Sensitive Polymer-Modified Liposomes for Induction of Antigen-Specific Immune Responses (2015)
  • Author(s): Eiji Yuba, Yuhei Kanda, Atsushi Harada, Kenji Kono
  • Organizer: 42nd Controlled Release Society Annual Meeting & Exposition
  • Place of Presentation: スコットランド、エジンバラ、Edinburgh International Conference Centre
  • Year and Date: 2015-07-26
  • Int'l Joint Research
• [Presentation] Presentation of tumor antigens in the context of MHC class II molecules by the endothelial cells in solid tumors grown in vivo (2015)
  • Author(s): Udaka K, Komatsu T, Ichikawa Y, Kuroe S
  • Organizer: ICCIM2015 International Conference of Cancer Immunotherapy and Macrophages 2015(がん免疫療法・マクロファージ国際会議2015)
  • Place of Presentation: 東京、東京大学
  • Year and Date: 2015-07-09
  • Int'l Joint Research
• [Presentation] pH応答機能と生理活性を持つ多糖誘導体修飾リポソームの作製とがん免疫治療への応用 (2015)
  • Author(s): 弓場英司, 山口彩加, 原田敦史, 河野健司
  • Organizer: 第31回日本DDS学会
  • Place of Presentation: 東京、京王プラザホテル
  • Year and Date: 2015-07-02
• [Presentation] 血管内皮細胞による腫瘍細胞の取り込みに関わるopsonin活性について (2015)
  • Author(s): 小松利広、宇高恵子
  • Organizer: 第１４回四国免疫フォーラム
  • Place of Presentation: 松山、愛媛大学
  • Year and Date: 2015-06-20
• [Presentation] Design of functional polymer-modified liposomes for antigen delivery and their application to cancer immunotherapy (2015)
  • Author(s): Eiji Yuba
  • Organizer: 第64回高分子学会年次大会
  • Place of Presentation: 札幌、札幌コンベンションセンター
  • Year and Date: 2015-05-27
  • Invited
• [Book] DDS先端技術の製剤への応用開発 (2017)
  • Author(s): 弓場英司
  • Total Pages: 450
  • Publisher: 技術情報協会
• [Book] DDSキャリア作製プロトコル集 (2015)
  • Author(s): 河野健司, 弓場英司
  • Total Pages: 262
  • Publisher: シーエムシー
• [Book] DDSキャリア作製プロトコル集 (2015)
  • Author(s): 弓場英司, 河野健司
  • Total Pages: 262
  • Publisher: シーエムシー
• [Remarks] 大阪府立大学大学院工学研究科応用化学分野生体高分子化学研究グループ
  • URL: http://www2.chem.osakafu-u.ac.jp/ohka/ohka9/index.htm
• [Remarks] 大阪府立大学大学院工学研究科応用化学分野生体高分子化学研究グループ
  • URL: http://www.chem.osakafu-u.ac.jp/ohka/ohka9/index.htm
• [Patent(Industrial Property Rights)] ｐＨ応答性リポソーム (2017)
  • Inventor(s): 弓場英司, 深谷佳樹
  • Industrial Property Rights Holder: 弓場英司, 深谷佳樹
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-136235
  • Filing Date: 2017
• [Patent(Industrial Property Rights)] 正電荷脂質と多糖誘導体を含む微粒子担体 (2016)
  • Inventor(s): 弓場英司 河野健司 門柚奈 小岩井一倫 坂口奈央樹
  • Industrial Property Rights Holder: 公立大学法人大阪府立大学 テルモ株式会社
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-214408
  • Filing Date: 2016-11-01