Development of Matrix Materials Mimicking Simultaneous Osteogenesis and Adipogenesis

• Project/Area Number: 15H03027
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: National Institute for Materials Science
• Principal Investigator: Chen Guoping 国立研究開発法人物質・材料研究機構, 機能性材料研究拠点, グループリーダー (50357505)
• Co-Investigator(Renkei-kenkyūsha): KAWAZOE Naoki 国立研究開発法人物質・材料研究機構, 機能性材料研究拠点, 主幹研究員 (90314848)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: マトリックス材料 / 生体模倣材料 / 足場材料 / 再生医療 / 幹細胞 / 再生医工学材料 / 組織発生模倣型マトリックス / ヒト間葉系幹細胞 / 骨分化 / 脂肪分化 / 組織発生模倣型マトリック

Outline of Final Research Achievements

The extracellular matrix (ECM) is dynamically remodeled to regulate stem cell functions during tissue development. Here, we have prepared novel types of matrices that mimic the dynamic variation of ECMs by using simultaneous osteogenesis and adipogenesis of mesenchymal stem cells (MSCs). The matrices had different effects on differentiation of MSCs due to the different compositions depending on the differentiation stage. Late osteogenesis and early adipogenesis (LOEA) and late osteogenesis and late adipogenesis (LOLA) matrices promoted osteogenesis but not adipogenesis. Early osteogenesis and early adipogenesis (EOEA) matrices promoted adipogenesis but not osteogenesis. Early osteogenesis and late adipogenesis (EOLA) did not promote either osteogenic or adipogenesis. The tissue development mimicking matrices can be prepared at large scale and thus should be useful as versatile biomaterials.

Research Products

• [Journal Article] 再生医療に用いる足場材料の必要条件と作製方法 (2018)
  • Author(s): 陳国平、川添直輝
  • Journal Title: PHARM STAGE Volume: 18巻 Pages: 3-7
• [Journal Article] 再生医療用多孔質足場材料の設計と作製 (2018)
  • Author(s): 陳国平、川添直輝
  • Journal Title: 生体材料 Volume: 36巻 Pages: 120-127
• [Journal Article] 軟骨および骨組織再生のための高分子多孔質足場材料 (2018)
  • Author(s): 川添直輝 、陳国平
  • Journal Title: バイオサイエンスとバイオインダストリー Volume: 72巻 Pages: 115-119
  • NAID: 40021512187
  • Peer Reviewed
• [Journal Article] Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells (2017)
  • Author(s): Takashi Hoshiba、Naoki Kawazoe、Guoping Chen
  • Journal Title: Methods in Molecular Biology Volume: VOL2017 Pages: 1-16
  • DOI: 10.1007/7651_2017_62
  • ISBN: 9781493976553, 9781493976560
  • Peer Reviewed
• [Journal Article] Biomimetic Assembly of Vascular Endothelial Cells and Muscle Cells in Microgrooved Collagen Porous Scaffolds (2017)
  • Author(s): Chen Shangwu、Kawazoe Naoki、and Chen Guoping
  • Journal Title: Tissue Engineering Part C-Methods Volume: VOL 23 Issue: 6 Pages: 367-376
  • DOI: 10.1089/ten.tec.2017.0088
  • Peer Reviewed
• [Journal Article] Induction of Chondrogenic Differentiation of Human Mesenchymal Stem Cells by Biomimetic Gold Nanoparticles with Tunable RGD Density (2017)
  • Author(s): Jingchao Li、Xiaomeng Li、Jing Zhang、Naoki Kawazoe、Guoping Chen
  • Journal Title: Advanced Healthcare Materials Volume: VOL 6 Issue: 14
  • DOI: 10.1002/adhm.201700317
  • Peer Reviewed
• [Journal Article] Preparation of cell-derived decellularized matrices mimicking native ECM during the osteogenesis and adipogenesis of mesenchymal stem cells (2017)
  • Author(s): T. Hoshiba, N. Kawazoe, and G. Chen
  • Journal Title: Methods in Molecular Biology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Matrices Secreted during Simultaneous Osteogenesis and Adipogenesis of Mesenchymal Stem Cells Affect Stem Cells Differentiation (2016)
  • Author(s): Rong Cai, Tomoko Nakamoto, Naoki Kawazoe, Takashi Hoshiba, Guoping Chen
  • Journal Title: Acta Biomaterialia Volume: 35 Pages: 185-193
  • DOI: 10.1016/j.actbio.2016.02.009
  • Peer Reviewed
• [Journal Article] Decellularized Extracellular Matrix as an In Vitro Model to Study the Comprehensive Roles of the ECM in Stem Cell Differentiation (2016)
  • Author(s): Takashi Hoshiba, Guoping Chen, Chiho Endo, Hiroka Maruyama, Miyuki Wakui, Eri Nemoto, Naoki Kawazoe, Masaru Tanaka
  • Journal Title: Stem Cells International Volume: 2016 Issue: 1 Pages: 6397820-6397820
  • DOI: 10.1155/2016/6397820
  • Peer Reviewed / Open Access
• [Presentation] 軟骨組織発生模倣型マトリックス足場材料の作製 (2018)
  • Author(s): 陳国平、 Cai Rong、 川添直輝
  • Organizer: 第17回日本再生医療学会総会
• [Presentation] Tissue Development-Mimicking Extracellular Matrices and Scaffolds for Stem Cells Culture (2017)
  • Author(s): Guoping Chen、 Naoki Kawazoe
  • Organizer: TERMIS-AM Meeting 2017
  • Int'l Joint Research / Invited
• [Presentation] Preparation of Biomimetic ECM Scaffolds and Application for Tissue Engineering (2017)
  • Author(s): Guoping Chen、 Naoki Kawazoe
  • Organizer: TERMIS-AP 2017
  • Int'l Joint Research / Invited
• [Presentation] Hybrid Scaffolds of Biodegradable Polymers and Biomimetic Matrices for Tissue Engineering Applications (2017)
  • Author(s): Guoping Chen、 Putri Nur Rofiqoh Eviana、 Naoki Kawazoe
  • Organizer: 12th Joint Conference on Chemistry
  • Int'l Joint Research / Invited
• [Presentation] Biodegradable and Biomimetic Scaffolds for Tissue Engineering (2017)
  • Author(s): Guoping Chen、 Naoki Kawazoe
  • Organizer: 2017 International Symposium of Materials on Regenerative Med
  • Int'l Joint Research / Invited
• [Presentation] Preparation of Extracellular Matrix-Derived Scaffolds for Tissue Engineering (2017)
  • Author(s): Guoping Chen、 Naoki Kawazoe
  • Organizer: Asia Pacific Society for Materials Research 2017 Annual Meeting
  • Int'l Joint Research / Invited
• [Presentation] Preparation of Biomimetic Matrices for Controlling Stem Cell Functions (2017)
  • Author(s): Guoping Chen、 Naoki Kawazoe
  • Organizer: 12th International Symposium on Frontiers in Biomedical Polymers
  • Int'l Joint Research / Invited
• [Presentation] Preparation of tissue development-mimicking ECM scaffolds for stem cell culture (2017)
  • Author(s): Guoping Chen、 Naoki Kawazoe
  • Organizer: TERMIS European Chapter Meeting 2017
  • Int'l Joint Research / Invited
• [Presentation] 再生医療のための足場材料技術 (2017)
  • Author(s): 陳国平
  • Organizer: ジャパンライフサイエンスウイーク2017
  • Invited
• [Presentation] Preparation of Polymeric Scaffolds and Biomimetic Matrices for Tissue Engineering (2016)
  • Author(s): Guoping Chen and Naoki Kawazoe
  • Organizer: 3rd International Conference on Biomaterials in Tokyo
  • Place of Presentation: 東京大学伊藤国際学術研究センター(東京都文京区)
  • Year and Date: 2016-11-28
  • Int'l Joint Research
• [Presentation] Biomimetic Matrices and Scaffolds from Cultured Cells (2016)
  • Author(s): Guoping Chen
  • Organizer: 2016 China-Korea Symposium on Biomimetic and Regenerative Medical Materials
  • Place of Presentation: 成都（中国）
  • Year and Date: 2016-11-18
  • Int'l Joint Research / Invited
• [Presentation] PREPARATION OF BIOMIMETIC ECM SCAFFOLDS FOR TISSUE ENGINEERING (2016)
  • Author(s): Guoping Chen and Naoki Kawazoe
  • Organizer: Biomaterials International 2016
  • Place of Presentation: 墾丁（台湾）
  • Year and Date: 2016-10-30
  • Int'l Joint Research / Invited
• [Presentation] Biodegradable Polymer Scaffolds and Biomimetic Matrices for Tissue Engineering Applications (2016)
  • Author(s): Guoping Chen
  • Organizer: HKU International Symposium for 3D Bio-printing and Biomaterials
  • Place of Presentation: 香港
  • Year and Date: 2016-10-27
  • Int'l Joint Research / Invited
• [Presentation] Design and Preparation of Hybrid and Biomimetic Porous Scaffolds for Tissue Engineering (2016)
  • Author(s): Guoping Chen, Rong Cai, Shangwu Chen, Naoki Kawazoe
  • Organizer: TERMIS-AP Meeting 2016
  • Place of Presentation: 新北（台湾）
  • Year and Date: 2016-09-03
  • Int'l Joint Research / Invited
• [Presentation] Preparation of Cultured Cell-Derived Biomimetic Scaffolds for Tissue Engineering (2016)
  • Author(s): Guoping Chen, Rong Cai, Naoki Kawazoe
  • Organizer: International Forum of Biomedical Materials-Biomaterials Interfaces and
  • Place of Presentation: 杭州（中国）
  • Year and Date: 2016-08-01
  • Int'l Joint Research / Invited
• [Presentation] Development of Biomimetic Scaffolds for Tissue Engineering (2016)
  • Author(s): Guoping Chen, Rong Cai, Naoki Kawazoe
  • Organizer: 2016 Asia University Symposium on Biomedical Engineering
  • Place of Presentation: 北京（中国）
  • Year and Date: 2016-07-20
  • Int'l Joint Research / Invited
• [Presentation] Extracellular matrices simultaneously mimicking stepwise osteogenesis-co-adipogenesis of mesenchymal stem cells (2016)
  • Author(s): Guoping Chen, Rong Cai, Naoki Kawazoe
  • Organizer: TERMIS-EU 2016
  • Place of Presentation: ウプサラ（スウェーデン）
  • Year and Date: 2016-06-28
  • Int'l Joint Research / Invited
• [Presentation] Polymer Porous Scaffolds and Biomimetic Matrices for Tissue Engineering (2016)
  • Author(s): Guoping Chen, Qin Zhang, Shangwu Chen, Rong Cai, Naoki Kawazoe
  • Organizer: Thailand-Japan Collaborative Seminar
  • Place of Presentation: バンコク（タイ）
  • Year and Date: 2016-05-25
  • Int'l Joint Research
• [Presentation] Tissue development-mimicking extracellular matrices from cultured cells (2016)
  • Author(s): Guoping Chen, Rong Cai, Takashi Hoshiba, Naoki Kawazoe
  • Organizer: 10th World Biomaterials Congress
  • Place of Presentation: モントリオール（カナダ）
  • Year and Date: 2016-05-17
  • Int'l Joint Research
• [Presentation] 細胞の機能制御及び組織再生のための足場材料 (2016)
  • Author(s): 陳 国平
  • Organizer: 学振第174委員会第54回研究会
  • Place of Presentation: 京都テルサ（京都府京都市）
  • Invited
• [Presentation] 再生医療のための組織発生模倣型マトリックス材料の作製 (2015)
  • Author(s): 陳国平, Cai Rong, 干場 隆志, 川添直輝
  • Organizer: 第64回高分子討論会
  • Place of Presentation: 東北大学川内キャンパス（宮城県仙台市）
  • Year and Date: 2015-09-15
• [Presentation] Influence of Stepwise Chondrogenesis-Mimicking 3D Extracellular Matrix on Chondrogenic Differentiation of Mesenchymal Stem Cells (2015)
  • Author(s): Cai Rong, 中本智子, 川添直輝, 陳国平
  • Organizer: 第44回医用高分子シンポジウム
  • Place of Presentation: 産業技術総合研究所 臨海副都心センター（東京都江東区）
  • Year and Date: 2015-07-27
• [Presentation] Preparation of Stepwise Chondrogenesis-Mimicking ECM Scaffolds for Tissue Engineering (2015)
  • Author(s): Guoping Chen, Rong Cai, Naoki Kawazoe
  • Organizer: 2nd International Conference on Regenerative Biomedical Materials
  • Place of Presentation: 武漢（中国）
  • Year and Date: 2015-06-04
  • Int'l Joint Research / Invited
• [Presentation] Stepwise Tissue Development-Mimicking ECM Scaffolds from Cultured Cells (2015)
  • Author(s): Guoping Chen, Rong Cai, Tomoko Nakamoto, Naoki Kawazoe
  • Organizer: 5th China-Europe Symposium on Biomaterials in Regenerative Medicine
  • Place of Presentation: 杭州（中国）
  • Year and Date: 2015-04-07
  • Int'l Joint Research / Invited
• [Book] 技術情報協会 (2017)
  • Author(s): 川添直輝、陳国平
  • Total Pages: 465
  • Publisher: 生体吸収性材料の開発と安全性評価（第2章1節）
  • ISBN: 9784861046896
• [Book] 技術情報協会 (2017)
  • Author(s): 陳国平、川添直輝
  • Total Pages: 526
  • Publisher: 動物細胞培養・自動化におけるトラブル発生原因と対策（第10章6節）
  • ISBN: 9784861046841
• [Book] Polymeric Biomaterials for Tissue Regeneration-From Surface/Interface Design to 3D Constructs (2016)
  • Author(s): Guoping Chen and Naoki Kawazoe
  • Publisher: Springer