Roles played by ribosome biogenesis in exercise-induced muscle hypertrophy

• Project/Area Number: 15H03078
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Sports science
• Research Institution: The University of Tokyo
• Principal Investigator: Ishii Naokata 東京大学, 大学院総合文化研究科, 教授 (20151326)
• Co-Investigator(Kenkyū-buntansha): 中里 浩一 日本体育大学, 保健医療学部, 教授 (00307993) ; 小笠原 理紀 名古屋工業大学, 工学(系)研究科(研究院), 准教授 (10634602)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: トレーニング / 筋肥大 / リボソーム量 / タンパク質合成 / 翻訳容量 / 翻訳効率 / mTORシグナル伝達系 / リボソーム生合成 / 翻訳活性化 / SUnSET法 / UBF / リボソーム合成 / 骨格筋肥大 / 動物モデル

Outline of Final Research Achievements

This study aimed to investigate the role played by ribosome biogenesis in exercise-induced muscle hypertrophy using the rat skeletal muscles. A compensatory hypertrophy model, with which the degree of hypertrophy could be controlled, showed that both the ribosomal content and the expression of UBF (upstream binding factor), a transcription factor for rRNA, are strongly correlated with the degree of hypertrophy. In a resistance-training model, the muscle hypertrophy after the exercise period for 6 weeks showed a close association with the increase in ribosomal content. The ribosomal content increased at an early stage of training period (within 1 wk. after the initiation of training), and changed following a different time course from those of mTOR signal transduction activity and protein synthesis. These results suggest that the ribosomal content changes dynamically with the training stimulus, and the ribosomal biogenesis plays an important part in exercise-induced muscle hypertrophy.

Research Products

• [Journal Article] Effects of defensive style Nordic walking intervention in patients with lumbar and lower-limb osteoarthritis. (2018)
  • Author(s): Fukusaki, C., Leetawesup, K., Kato, N., Kadokura, Y., Nakazawa, K., Yano, H., Ishii, N.
  • Journal Title: J. Nov. Phsyiother. Volume: 8-383 Issue: 02 Pages: 1-8
  • DOI: 10.4172/2165-7025.1000383
  • Peer Reviewed / Open Access
• [Journal Article] Post-exercise blood restriction does not enhance muscle hypertrophy induced by multiple-set high-load resistance exercise. (2018)
  • Author(s): Madarame, H., Nakada, S., Ohta, T., Ishii, N.
  • Journal Title: Clin. Physiol. Funct. Imaging Volume: 18 Pages: 360-365
  • Peer Reviewed
• [Journal Article] Influence of past injurious exercise on fiber-type specific acute anabolic responses to resistance exercise in skeletal muscle. (2018)
  • Author(s): Takagi, R., Ogasawara, R., Takegaki, J., Tsutaki, A., Nakazato, K., Ishii, N.
  • Journal Title: J. Appl. Phyisiol. Volume: in press
  • Peer Reviewed
• [Journal Article] Electrically evoked local muscle contractions cause an increase in hippocampal BDNF. (2018)
  • Author(s): Maekawa, T., Ogasawara, R., Tsutaki, A., Lee, K., Nakada, S., Nalazato K., Ishii, N.
  • Journal Title: Appl. Physiol. Nutr. Metab. Volume: in press Issue: 5 Pages: 1-6
  • DOI: 10.1139/apnm-2017-0536
  • Peer Reviewed
• [Journal Article] Repeated bouts of resistance exercise with short recovery periods activates mTOR signaling, but not protein synthesis, in mouse skeletal muscle (2017)
  • Author(s): Junya Takegaki, Riki Ogasawara, Yuki Tamura, Ryo Takagi, Yuki Arihara, Arata Tsutaki, Koichi Nakazato, Naokata Ishii
  • Journal Title: Physiological Reports Volume: 5 Issue: 22
  • DOI: 10.14814/phy2.13515
  • Peer Reviewed / Open Access
• [Journal Article] Relationship between exercise volume and muscle protein synthesis in a rat model of resistance exercise. (2017)
  • Author(s): Ogasawara, R., Arihara, Y., Takegaki, J., Nakazato, K., Ishii, N.
  • Journal Title: J. Appl. Physiol. Volume: 123 Pages: 710-716
  • Peer Reviewed
• [Journal Article] Correlation between ribosome biogenesis and the magnitude of hypertrophy in overloaded skeletal muscle. (2016)
  • Author(s): Nakada, S., Ogasawara, R., Kawada, S., Maekawa, T. and Ishii, N.
  • Journal Title: PLoS ONE Volume: 11 Issue: 1 Pages: e0147284-e0147284
  • DOI: 10.1371/journal.pone.0147284
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The role of mTOR signalling in the regulation of skeletal muscle mass in a rodent model of resistance exercise. (2016)
  • Author(s): Ogsawara, R., Fujita, S., Hornberger, T., Kitaoka, Y., Makanae, Y., Nakazato, K. and Ishii, N.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 31142-31142
  • DOI: 10.1038/srep31142
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Regional adaptation of collagen in skeletal muscle to repeated bouts of strenuous eccentric exercise. (2016)
  • Author(s): Takagi, R., Ogasawara, R., Tsutaki, A., Nakazato, K. and Ishii, N.
  • Journal Title: Pflugers Archiv (Eur. J. Physiol.) Volume: 468 Issue: 9 Pages: 1565-1572
  • DOI: 10.1007/s00424-016-1860-3
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effect of resistance training using bodyweight in the elderly:Comparison of resistance exercise movement between slowand normal speed movement. (2015)
  • Author(s): Wtanabe, Y., Tanimoto, M., Oba, N., Sanada, K., Miyachi, M., Ishii, N.
  • Journal Title: Geriatric and Gerontology International Volume: 15 Issue: 12 Pages: 1270-1277
  • DOI: 10.1111/ggi.12427
  • Peer Reviewed / Open Access
• [Journal Article] 骨格筋と熱産生 (2015)
  • Author(s): 石井直方
  • Journal Title: 臨床スポーツ医学 Volume: 32 Pages: 1032-1039
• [Presentation] Consective bouts of resistance exercise cause increase in ribosomes in rat training model. (2017)
  • Author(s): Takaya Kotani, Junya Takegaki, Satoshi Nakada, Ryo Takagi, Shigeto Tomiya, Arata Tsutaki, Koichi Nakazato, Naokata Ishii
  • Organizer: 5th NSCA International Coference
  • Place of Presentation: 幕張メッセ（千葉県、千葉市美浜区中瀬）
  • Year and Date: 2017-01-29
  • Int'l Joint Research
• [Presentation] Ribosome biogenesis is activated during the early period of resistance training in rat skeletal muscle. (2017)
  • Author(s): Kotani, T., Takegaki, J., Nakada, S., Takagi, R., Tsutaki, A., Nakazato, K., Ishii, N.
  • Organizer: Experimental Biology 2017
  • Int'l Joint Research
• [Presentation] Changes in ribosome content during early and middle period of resistance training in rat skeletal muscle. (2017)
  • Author(s): Kotani, T., Takegaki, J., Takagi, R. Tsutaki, A., Nakazato, K., Ishii, N.
  • Organizer: Interdisciplinary Symposium for Up-and-coming Material Scientists 2017
  • Int'l Joint Research
• [Presentation] Resistance training with short interval between bouts does not induce skeletal muscle hypertrophy in rats. (2017)
  • Author(s): Takegaki, J., Kotani, T., Ogasawara, R., Tsutaki, A., Nakazato, K., Ishii, N.
  • Organizer: Experimental Biology 2017
  • Int'l Joint Research
• [Presentation] High frequency resistance training does not cause skeletal muscle hypertrophy due to activation of proteolytic systems in rat skeletal muscle. (2017)
  • Author(s): Takegaki, J., Ogasawara, R., Tsutaki, A., Nakazato, K., Ishii, N.
  • Organizer: Cell symposia: Exercise Metabolism
  • Int'l Joint Research
• [Presentation] セッション間の回復時間の違いが同強度・同回数のレジスタンス運動の効果に与える影響 (2017)
  • Author(s): 竹垣 淳也，小谷 鷹哉，小笠原 理紀，蔦木 新，中里 浩一，石井直方
  • Organizer: 第25回日本運動生理学会大会
• [Presentation] レジスタンストレーニング初期におけるリボソーム合成に関わる因子の変化 (2017)
  • Author(s): 小谷 鷹哉, 竹垣 淳也, 高木 領, 東宮 繁人, 蔦木 新, 中里 浩一, 石井 直方
  • Organizer: 第25回日本運動生理学会大会
• [Presentation] セッション間の回復時間の違いがレジスタンス運動後の筋タンパク質分解系に与える影響 (2017)
  • Author(s): 竹垣 淳也，小谷 鷹哉，小笠原 理紀，蔦木 新， 中里 浩一，石井直方
  • Organizer: 第72回日本体力医学会大会
• [Presentation] 継続的なレジスタンストレーニングによるリボソーム量の増加メカニズムの検討 (2017)
  • Author(s): 小谷 鷹哉, 竹垣 淳也, 高木 領, 東宮 繁人, 蔦木 新, 中里 浩一, 石井 直方
  • Organizer: 第72回日本体力医学会大会
• [Presentation] Effect of increasing exercise bout on skeletal muscle ribosome biogenesis. (2016)
  • Author(s): Takaya Kotani, Satoshi Nakada, Ryo Takagi, Junya Takegaki, Shigeto Tomiya, Arata Tsutaki, Koichi Nakazato, Naokata Ishii
  • Organizer: 10th International Conference on Strength Training
  • Place of Presentation: 龍谷大学響都ホール （京都府、京都市南区東九条西山王町）
  • Year and Date: 2016-11-30
  • Int'l Joint Research
• [Presentation] Fiber type-dependent differences in anabolic response of skeletal muscle to resistance exercise. (2016)
  • Author(s): Takagi R, Ogasawara R, Takegaki J, Tsutaki A, Nakazato K, Ishii N.
  • Organizer: The Integrative Biology of Exercise VII
  • Place of Presentation: Phoenix, USA
  • Year and Date: 2016-11-02
  • Int'l Joint Research
• [Presentation] レジスタンストレーニング回数の違いがリボソーム生合成におよぼす影響 (2016)
  • Author(s): 小谷鷹哉、 中田智史、竹垣淳也、 高木領、 東宮繁人、蔦木新、 中里浩一、 石井直方
  • Organizer: 第71回 日本体力医学会
  • Place of Presentation: いわて県民情報交流センター（岩手県、盛岡市盛岡駅西通）
  • Year and Date: 2016-09-25
• [Presentation] Repeated bouts of resistance training activate Akt-mTOR signaling, but not protein synthesis in mouse skeletal muscle. (2016)
  • Author(s): Junya Takegaki, Yuki Arihara, Riki Ogasawara, Arata Tsutaki, Koichi Nakazato, Naokata Ishii
  • Organizer: Experimental Biology 2016
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-04-04
  • Int'l Joint Research
• [Presentation] Repeated bouts of resistance training activate Akt-mTOR signaling, but not protein synthesis in mouse skeletal muscle. (2016)
  • Author(s): Junya Takegaki, Yuki Arihara, Riki Ogasawara, Arata Tsutaki, Koichi Nakazato, Naokata Ishii
  • Organizer: Experimental Biology 2016
  • Place of Presentation: サンディエゴ（アメリカ合衆国）
  • Year and Date: 2016-04-04
  • Int'l Joint Research
• [Presentation] レジスタンストレーニングにおける回復時間の違いが筋タンパク合成に与える影響 (2016)
  • Author(s): 竹垣淳也，石井直方
  • Organizer: 第4回骨格筋生物学研究会
  • Place of Presentation: 松本大学（長野県松本市）
  • Year and Date: 2016-03-04
• [Presentation] 伸張性収縮による骨格筋損傷後の筋線維ミオシン重鎖タイプ構成 (2015)
  • Author(s): 高木領, 小笠原理紀, 蔦木新, 中里浩一, 石井直方
  • Organizer: 第70回日本体力医学会大会
  • Place of Presentation: 和歌山県民文化会館（和歌山県和歌山市）
  • Year and Date: 2015-09-18
• [Book] Stiffness of Contracting Human Muscle Measured with Supersonic Shear Imaging. In “Muscle Contraction and Cell Motility: Fundamentals and Developments” (Sugi, H., ed.) (2016)
  • Author(s): Sasaki, K. and Ishii, N.
  • Publisher: Pan Stanford Publishing, Singapore
• [Remarks] 東京大学石井直方教授の情報サイト
  • URL: http://wildlife.jpn.com/wildlife-consul/ishii/content/labo/index.html
• [Remarks] 東京大学 石井直方教授の情報サイト
  • URL: http://wildlife.jpn.com/wildlife-consul/ishii/content/labo/index.html