| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we used 3 complementary studies: i) C2C12 myotubes were subjected to uniaxial stretch to assess whether stretching regulates p62 expression; ii) C57BL/6 mice were assigned to either voluntary running or no exercise to examine whether exercise training regulates p62 protein; and iii) muscle-specific p62 knockout and wild-type littermates mice were assigned to either exercise training or sedentary groups, and the muscle content of antioxidants was determined after exercise training. Our results demonstrated that p62 mRNA levels were significantly increased by mechanical stretch in C2C12 myotubes. Voluntary running in C57BL/6 mice increased p62 protein and p62 phosphorylation in skeletal muscle. Loss of p62 in skeletal muscle also blunted the exercise training-mediated increase in antioxidants in skeletal muscle. These findings indicate that p62 is required for exercise training-mediated increase of antioxidants in skeletal muscle.
|
