Development of various functional peptides for biochips analyzing cancer cells

• Project/Area Number: 15H03113
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomolecular chemistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Mihara Hisakazu 東京工業大学, 生命理工学院, 教授 (30183966)
• Co-Investigator(Kenkyū-buntansha): 堤 浩 東京工業大学, 生命理工学院, 准教授 (70398105)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 生体分子科学 / ペプチド / 細胞相互作用 / バイオチップ

Outline of Final Research Achievements

In order to approach the development of peptide biochips analyzing properties of various cancer cells and stem cells, the construction of a variety of functional peptides interacting with cells has been performed. The peptide biochip system utilizes the cell fingerprint analyses for characterization of cell properties.

Research Products

• [Journal Article] Gold Nanoparticles Conjugated with Glycopeptides for Lectin Detection and Imaging on Cell Surface (2018)
  • Author(s): Hiroshi Tsutsumi, Tomoko Shirai, Hiroyuki Ohkusa, Hisakazu Mihara
  • Journal Title: Protein Peptide Letter Volume: 25 Issue: 1 Pages: 84-89
  • DOI: 10.2174/0929866525666171218124434
• [Journal Article] Screening for Concanavalin A Binders from a Mannose-modified alpha-Helix Peptide Phage Library (2017)
  • Author(s): Iou Ven Chang, Hiroshi Tsutsumi, Hisakazu Mihara
  • Journal Title: Molecular BioSystems Volume: 13 Issue: 11 Pages: 2222-2225
  • DOI: 10.1039/c7mb00495h
  • Peer Reviewed
• [Journal Article] Dihydrofolate Reductase Inhibitory Peptides Screened from a Structured Designed β-Loop Peptide Library Displayed on Phage (2015)
  • Author(s): Hiroshi Tsutsumi, Kazuhiro Nakano, Hisakazu Mihara
  • Journal Title: Molecular BioSystems Volume: 11 Issue: 10 Pages: 2713-2716
  • DOI: 10.1039/c5mb00316d
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Monosaccharide-modified alpha-helix peptide phage libraries for carbohydrate-binding proteins (2018)
  • Author(s): Iou Ven Chang, Hiroshi Tsutsumi, Hisakazu Mihara
  • Organizer: 日本化学会第98春季年会
• [Presentation] Semisynthetic approach for ligand screening using chemically modified peptide phage libraries (2017)
  • Author(s): Hiroshi Tsutsumi, Iou Ven Chang, Teerapat Anananuchatkul, Hisakazu Mihara
  • Organizer: 第54回ペプチド討論会
  • Int'l Joint Research
• [Presentation] 二次構造設計糖ペプチドファージライブラリの構築とレクチン結合性糖ペプチドリガンド探索への応用 (2017)
  • Author(s): 堤浩、Chang IouVen、廣原庸平、三原久和
  • Organizer: 日本化学会第97春季年会
• [Presentation] 両親媒性αヘリックスペプチドの細胞導入特性の解析 (2017)
  • Author(s): 澁谷圭祐、陶欣然、堤浩、三原久和
  • Organizer: 日本化学会第97春季年会
• [Presentation] 単糖導入ペプチド修飾金ナノ粒子の合成とレクチンタンパク質との相互作用 (2016)
  • Author(s): 白井智子、堤 浩、湯浅英哉、三原久和
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 京都
  • Year and Date: 2016-03-24
• [Presentation] 細胞識別に向けた種々の両親媒性α-ヘリックスペプチドの細胞膜透過活性解析 (2016)
  • Author(s): 堤浩、陶欣然、三原久和
  • Organizer: 第10回バイオ関連化学シンポジウム
• [Presentation] Screening for concanavalin A binders from a mannose-conjugated alpha-helix peptide phage library (2016)
  • Author(s): IouVen Chang, Hiroshi Tsutsumi, Hisakazu Mihara
  • Organizer: 第53回ペプチド討論会
  • Int'l Joint Research
• [Presentation] A mannose-conjugated α-helix designed peptide phage-library as a tool for the screening of Concanavalin A binding ligands (2015)
  • Author(s): IouVen Chang、Yohei Hirohara、Hiroshi Tsutsumi、Hisakazu Mihara
  • Organizer: The 7th International Peptide Symposium
  • Place of Presentation: Singapore
  • Year and Date: 2015-12-09
  • Int'l Joint Research
• [Presentation] 細胞選択的な膜透過性を有する設計α-ヘリックスペプチドの機能解析 (2015)
  • Author(s): 堤 浩、陶欣然、三原久和
  • Organizer: 第9回バイオ関連化学シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 2015-09-10
• [Presentation] 単糖修飾β-ループペプチドファージライブラリの構築と糖結合タンパク質結合性ペプチドの探索 (2015)
  • Author(s): 廣原庸平、新井佳菜子、堤 浩、三原久和
  • Organizer: 第25回バイオ・高分子シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2015-07-23
• [Book] ペプチド医薬品のスクリーニング・安定化・製剤化技術 (2017)
  • Author(s): 堤浩、三原久和
  • Total Pages: 557
  • Publisher: 技術情報協会
  • ISBN: 9784861046872
• [Book] 医療・診断をささえるペプチド科学－再生医療・DDS・診断への応用 (2017)
  • Author(s): 堤浩、三原久和
  • Total Pages: 315
  • Publisher: シーエムシー出版
  • ISBN: 9784781312675
• [Book] Peptide Microarrays-Method and Protocols (2016)
  • Author(s): Kenji Usui, Kinya Tomizaki, Hisakazu Mihara
  • Total Pages: 296
  • Publisher: Springer
  • ISBN: 9781493930371
• [Book] バイオチップの基礎と応用－原理から最新の研究・開発動向まで－ (2015)
  • Author(s): 臼井健二、堤 浩、三原久和
  • Total Pages: 275
  • Publisher: シーエムシー出版