| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Outline of Final Research Achievements |
Recent studies demonstrated that lipid molecules interact with membrane proteins and regulate their structures and functions. This makes us to realize the biological significance of lipids. However, due to the lack of conventional method to evaluate the interaction between lipids and membrane proteins, lipid research based on chemical biology has not been fully developed. Hence, in this study, we aimed at the creation of a new research field “lipid chemical biology”. We devised lipid-immobilized beads to identify lipid-specific membrane proteins, developed a SPR-based concise method for quantitative analysis of interaction between lipids and membrane proteins, and synthesized various fluorescent lipids to detect the interaction with membrane proteins using fluorescent microscope. In this way we successfully constructed technical bases for lipid chemical biology research.
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