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Construction of a high-speed structured illumination microscope and its application to the observation of dynamic soft materials

Research Project

Project/Area Number 15H03518
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nanostructural chemistry
Research InstitutionTohoku University

Principal Investigator

Kajimoto Shinji  東北大学, 薬学研究科, 講師 (80463769)

Co-Investigator(Kenkyū-buntansha) 福村 裕史  東北大学, 理学研究科, 客員教授 (50208980)
Co-Investigator(Renkei-kenkyūsha) SOGAWA Kazuhiro  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Keywords構造化照明顕微鏡 / ソフトマテリアル / 超解像イメージング / 蛍光 / オプティカルセクショニング / 超解像顕微鏡 / ナノ構造 / 細胞 / 相分離 / 溶液内柔構造 / 構造化照明 / 顕微鏡 / ソフトナノマテリアル / Ouzo効果 / 相分離構造
Outline of Final Research Achievements

By combining a high-repetition-rate nanosecond pulsed laser as a light source, and a high-speed camera as a detector, we constructed a fast structured illumination microscope. A laser pulse was split into two beam lines by using a grating and the structured illumination pattern was obtained on a sample as the interference pattern of two or three beams. The introduction of two Pockels' cells into the beam line(s) enables us to modulate the phase of the illumination pattern electrically within few hundreds microseconds. By the high-speed camera synchronized with the Pockels' cells, we obtained fluorescence images illuminated with different-phase pattern. As the result, we succeeded in obtaining optical sectioned images of a fluorescent stained living cell every 3 ms, and super-resolution images within 5 ms.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (19 results)

All 2018 2017 2016 2015

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Single bovine serum albumin molecule can hold plural blue-emissive gold nanoclusters: A quantitative study with two-photon excitation2018

    • Author(s)
      Hamza Al Kindi, Ahmed Mohamed, Shinji Kajimoto, Nurbosyn Zhanpeisov, Hideyuki Horino, Yutaka Shibata, Izabela I. Rzeznicka, Hiroshi Fukumura
    • Journal Title

      J. Photochem. Photobiol. A

      Volume: 357 Pages: 168-174

    • DOI

      10.1016/j.jphotochem.2018.02.029

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Experimental Evaluation of Density of Water in a Cell by Raman Microscopy2017

    • Author(s)
      Mizuki Takeuchi, Shinji Kajimoto, Takakazu Nakabayashi
    • Journal Title

      The Journal of Physical Chemistry Letters

      Volume: 8 Issue: 21 Pages: 5241-5245

    • DOI

      10.1021/acs.jpclett.7b02154

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Formation and Growth of Silver Nanocubes upon Nanosecond Pulsed Laser Irradiation: Effects of Laser Intensity and Irradiation Time2017

    • Author(s)
      Umair Yaqub Qazi, Zameer Shervani, Rahat Javaid, Shinji Kajimoto, and Hiroshi Fukumura
    • Journal Title

      Advances in Nanoparticles

      Volume: 6 Issue: 04 Pages: 148-157

    • DOI

      10.4236/anp.2017.64013

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Conformational changes in inhibitory PAS domain protein associated with binding of HIF-1α and Bcl-xL in living cells2017

    • Author(s)
      S. Kasai, S. Kajimoto, Y. Ito, T. Saito, K. Yasumoto, M. Tokunaga, K. Sakata-Sogawa, H. Fukumura, K. Sogawa
    • Journal Title

      J. Biochem.

      Volume: 161 Pages: 291-296

    • DOI

      10.1093/jb/mvw068

    • NAID

      40021162091

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chlorine adlayer-templated growth of a hybrid inorganic - organic layered structure on Au(111)2016

    • Author(s)
      I.I. Rzeznicka, H. Horino, K. Yagyu, T. Suzuki, S. Kajimoto, H. Fukumura
    • Journal Title

      Surface Science

      Volume: 印刷中 Pages: 46-50

    • DOI

      10.1016/j.susc.2016.03.007

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Phase behavior of a binary fluid mixture of quadrupolar molecules2016

    • Author(s)
      M. Toda, S. Kajimoto, S. Toyouchi, T. Kawakatsu, Y. Akama, M. Kotani, H. Fukumura
    • Journal Title

      Phys. Rev. E,

      Volume: 94 Issue: 5 Pages: 052601-052601

    • DOI

      10.1103/physreve.94.052601

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] Molecular behavior in biological systems studied by various microscopic techniques2017

    • Author(s)
      Shinji Kajimoto
    • Organizer
      化学系学協会東北大会/Joint Meeting of the Tohoku Area Chemistry Societies
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 蛍光及びラマン顕微鏡によって観測される細胞内の分子挙動2017

    • Author(s)
      梶本真司
    • Organizer
      日本薬学会東北支部主催 第6回物理・分析系若手研究者セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 金ナノ粒子の圧力に依存した発光における粒子サイズの効果2017

    • Author(s)
      松本季大,梶本真司,福村裕史,橋本修一
    • Organizer
      2017年光化学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 生細胞の細胞膜付近における分子の動態の解明に向けた全反射ラマン/蛍光顕微鏡の構築2017

    • Author(s)
      炭谷双葉, 梶本真司, 中林孝和
    • Organizer
      第11回分子科学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 超解像での生細胞観察に向けた高速構造化照明顕微鏡の構築2017

    • Author(s)
      鈴木斗夢, 梶本真司, 中林孝和
    • Organizer
      第11回分子科学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ラマンイメージングによる生細胞内の水の定量2017

    • Author(s)
      竹内瑞貴, 梶本真司, 中林孝和
    • Organizer
      第11回分子科学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Synthesis of chiral compounds in transient mesoscopic structures formed during laser-induced phase separation2016

    • Author(s)
      Shinji KAJIMOTO
    • Organizer
      1st International Symposium on Photosynergetics
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 金ナノ粒子のナノ秒レーザーパルス励起に伴う発光現象における圧力依存性2016

    • Author(s)
      松本季大,梶本真司,福村裕史,橋本修一
    • Organizer
      2016年光化学討論会
    • Related Report
      2016 Annual Research Report
  • [Presentation] ジアセチレンナノ結晶固相光重合初期過程のサブピコ秒過渡吸収分光2016

    • Author(s)
      仮屋薗 寛悟,梅本 嵩之,小野寺 恒信,及川 英俊,五月女 光,梶本 真司,福村 裕史,上田 潔,和田 真一,平谷 篤也
    • Organizer
      第10回分子科学討論会
    • Related Report
      2016 Annual Research Report
  • [Presentation] iso-ブトキシエタノール-水混合溶液のナノ秒温度ジャンプ誘起相分離過程2016

    • Author(s)
      深井隆達,横須賀巧,梶本真司,福村裕史
    • Organizer
      第10回分子科学討論会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Plasmon-mediated formation and growth of gold nanoparticles on ITO substrates2015

    • Author(s)
      Shinji KAJIMOTO, Takashi OKAMOTO, Tomonori TAKAHASHI, Hiroshi FUKUMURA,
    • Organizer
      The International Chemical Congress Of Pacific Basin Societies 2015 (PacifiChem2015)
    • Place of Presentation
      Hawaii
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ouzo効果を示す水-エタノール-オクタノール三成分系溶液のラマンスペクトル2015

    • Author(s)
      神山貴大、梶本真司、福村裕史
    • Organizer
      第9回分子科学討論会
    • Place of Presentation
      東京
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Book] “Raman imaging Microscopy for Quantitative Analysis of Biological Samples”, in Multi-Parametric Live Cell Microscopy of 3D Tissue Models2017

    • Author(s)
      Shinji Kajimoto, Mizuki Takeuchi, Takakazu Nakabayashi
    • Total Pages
      10
    • Publisher
      Springer
    • ISBN
      9783319673585
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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