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Development and application of a high speed online electron tomography system with a non-linear discrete reconstruction method unaffected by the missing data range

Research Project

Project/Area Number 15H03538
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nanomaterials engineering
Research InstitutionKogakuin University

Principal Investigator

Baba Norio  工学院大学, 情報学部(情報工学部), 教授 (80164896)

Co-Investigator(Kenkyū-buntansha) 馬場 美鈴  工学院大学, 総合研究所(付置研究所), 研究員 (80435528)
Co-Investigator(Renkei-kenkyūsha) Kaneko Kenji  九州大学, 大学院・工学研究院, 教授 (30336002)
Research Collaborator Ogasawara Mitsuo  工学院大学, 総合研究所, 研究員
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Keywords電子線トモグラフィ / 電子顕微鏡 / 逆問題 / 画像再構成 / 情報欠落問題 / ナノ材料 / ナノバイオ
Outline of Final Research Achievements

Electron tomography based on the same principle as the medical X-ray CT is widely applied to various fields of biological and material science for 3D structural analysis with nano-order resolution. However, because in general the projection images of all directions are not acquired from the reason of instrumental specialty, the depth resolution is considerably low. In addition, it takes more than one hour to obtain tomographic images, which is serious problem from the viewpoint of practical use. In this research, to resolve these problems the efficiency of a uniquely devised reconstruction method has further improved. As a result, the depth resolution has been recovered enough. Furthermore, an online CT system has been experimentally made ahead of the world, which is capable of auto-focusing, auto-tracking of the field of view and tomographic reconstruction even for a small number of tilt series images.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (29 results)

All 2018 2017 2016 2015 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (24 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Spot auto-focusing and spot auto-stigmation methods with high-definition auto-correlation function in high-resolution TEM2018

    • Author(s)
      Shigeto Isakozawa Taishi Fuse Junpei Amano Norio Baeeba
    • Journal Title

      Microscopy

      Volume: 67 Issue: 2 Pages: 75-88

    • DOI

      10.1093/jmicro/dfy001

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 電子顕微鏡画像処理による3次元断層法と表面構造復元法2017

    • Author(s)
      馬場則男
    • Journal Title

      「画像ラボ」、日本工業出版

      Volume: 9月号 Pages: 57-61

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Design of a 300-kV gas environmental transmission electron microscope equipped with a cold field emission gun2016

    • Author(s)
      S.Isakozawa,I.Nagaoki,A.Watabe,Y.Nagakubo,N.Saito,H.Matsumoto,X.F.Zhang,Y.Taniguchi and N.Baba
    • Journal Title

      Microscopy

      Volume: 65 Issue: 4 Pages: 353-362

    • DOI

      10.1093/jmicro/dfw015

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] 電子線トモグラフィ高精度化画像処理法と新奇な断層像再構成法の試み2017

    • Author(s)
      馬場則男
    • Organizer
      日本顕微鏡学会 マルチディメンジョナルトモグラフィ研究部会
    • Place of Presentation
      東北大学片平キャンパス
    • Year and Date
      2017-02-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 濃度量子単位に基づく非線形離散再構成法の最適化の改善2017

    • Author(s)
      馬場 則男,増本 奉之,馬場 美鈴
    • Organizer
      日本顕微鏡学会第73回学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 高精細自己相関関数による電子顕微鏡のスポット オートフォーカス及びオートスティグマ2017

    • Author(s)
      天野 純平,坂本 祥平,手塚 聖貴,馬場 美鈴, 久保 貴,砂子沢 成人,馬場 則男
    • Organizer
      日本顕微鏡学会第73回学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 機械学習による電子顕微鏡像のセグメンテーション及び輪郭線抽出手法の提案2017

    • Author(s)
      前田 元,手塚 聖貴,坂本 祥平,馬場 美鈴, 馬場 則男
    • Organizer
      日本顕微鏡学会第73回学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 自己符号化器の改良と事前学習の効率を上げる HOG特徴解析法による粒子自動抽出2017

    • Author(s)
      手塚 聖貴,前田 元,馬場 美鈴,馬場 則男
    • Organizer
      日本顕微鏡学会第73回学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 電子線トモグラフィにおける最近の再構成法の進展と新奇な濃度量子単位に基づく再構成法2017

    • Author(s)
      馬場則男、増本 奉之、馬場 美鈴
    • Organizer
      日本顕微鏡学会第60回記念シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Segmentation and Contour Extraction in Biological Transmission Electron Microscope Images with ‘Bag-of-Features’ Method in Machine Learning2017

    • Author(s)
      Gen Maeda, Shoki Tezuka, Shohei Sakamoto, Misuzu Baba and Norio Baba
    • Organizer
      Microscopy and Microanalysis,Vol.23 No.S1, pp.138-139, (2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A particle extraction method with an improved auto-encoder in neural networks with the aid of HOG feature analysis2017

    • Author(s)
      Shoki Tezuka, Gen Maeda, Misuzu Baba and Norio Baba
    • Organizer
      Microscopy and Microanalysis,Vol.23 No.S1, pp.134-135, (2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A non-linear discrete reconstruction method based on the gray-level quantization unit2016

    • Author(s)
      Tomoyuki Masumoto, Misuzu Baba, Norio Baba
    • Organizer
      The 59th Symposium of The Japanese Society of Microscopy
    • Place of Presentation
      帝京平成大学 池袋キャンパス
    • Year and Date
      2016-11-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] Contour detection and segmentation method applicable to Electron tomography images with auto-classification by machine learning2016

    • Author(s)
      Gen Maeda, Shoki Tezuka, Shohei Sakamoto, Misuzu Baba, Norio Baba
    • Organizer
      The 59th Symposium of The Japanese Society of Microscopy
    • Place of Presentation
      帝京平成大学 池袋キャンパス
    • Year and Date
      2016-11-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] A spot auto-focus method with high definition ACF and applications to electron tomography2016

    • Author(s)
      Shohei Sakamoto,, Kenta Sugimoto, Tomoyuki Masumoto, Shoki Tezuka, Misuzu Baba and Norio Baba
    • Organizer
      The 59th Symposium of The Japanese Society of Microscopy
    • Place of Presentation
      帝京平成大学 池袋キャンパス
    • Year and Date
      2016-11-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] Applications of a particle extraction method with deep neural networks using improved auto-encoders to biological ultra-thin section images2016

    • Author(s)
      Shoki Tezuka, Gen Maeda, Misuzu Baba, Norio Baba
    • Organizer
      The 59th Symposium of The Japanese Society of Microscopy
    • Place of Presentation
      帝京平成大学 池袋キャンパス
    • Year and Date
      2016-11-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] 濃度量子単位に基づく非線形離散濃度階調再構成法の総当たり法による改善2016

    • Author(s)
      馬場 則男,増本 奉之,馬場 美鈴
    • Organizer
      日本顕微鏡学会第72回学術講演会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2016-06-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] ニューラルネットワークにおける自己符号化器を用いた自動粒子抽出2016

    • Author(s)
      手塚聖貴,前田 元,馬場美鈴,馬場 則男
    • Organizer
      日本顕微鏡学会第72回学術講演会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2016-06-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 3D相関視野追跡法とHD-ACFスポットオートフォーカスによるオンライントモグラフィ2016

    • Author(s)
      坂本 祥平,杉本 健太,増本 奉之,手塚 聖貴,馬場 美鈴,馬場 則男
    • Organizer
      日本顕微鏡学会第72回学術講演会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2016-06-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 積算平均ラインプロファイルマップを用いた電子顕微鏡画像上の輪郭線抽出法2016

    • Author(s)
      前田 元,手塚 聖貴,馬場 美鈴,馬場 則男
    • Organizer
      日本顕微鏡学会第72回学術講演会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2016-06-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 濃度量子単位に基づく非線形離散濃度階調再構成法の実用化2016

    • Author(s)
      増本 奉之,馬場 美鈴,馬場 則男
    • Organizer
      日本顕微鏡学会第72回学術講演会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2016-06-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] オートファジー不能変異株の形態学的解析2016

    • Author(s)
      馬場 美鈴,坂本 祥平,前田 元,手塚 聖貴,馬場則男
    • Organizer
      日本顕微鏡学会第72回学術講演会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2016-06-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] Image enhancement of electron microscope images with Gabor wavelets-based filtering2015

    • Author(s)
      Tomoyuki Masumoto, Gen Maeda and Norio Baba
    • Organizer
      The 2nd East-Asia Microscopy Conference
    • Place of Presentation
      The Himeji Chamber of Commerce and Industry
    • Year and Date
      2015-11-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 濃度量子化単位に基づく非線形離散濃度階調再構成法の収束性の改善2015

    • Author(s)
      馬場則男
    • Organizer
      日本顕微鏡学会第71回学術講演会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2015-05-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] ‘逆投影線像’相互相関による自動視野追跡法の広角度傾斜追跡性能2015

    • Author(s)
      坂本 祥平,杉本 健太,友永 祥彦,馬場 美鈴,馬場 則男
    • Organizer
      日本顕微鏡学会第71回学術講演会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 3次元逆投影線像相互相関法による投影シリーズの倍率補正2015

    • Author(s)
      杉本 健太,友永 祥彦,馬場 美鈴,馬場 則男
    • Organizer
      日本顕微鏡学会第71回学術講演会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] ガボールウェーブレットフィルターを用いた電子顕微鏡画像強調処理2015

    • Author(s)
      増本 奉之,前田 元,馬場 則男
    • Organizer
      日本顕微鏡学会第71回学術講演会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] オートファゴソーム前駆体膜が積荷を選択的に捕捉する機構の3次元形態解析2015

    • Author(s)
      馬場 美鈴,杉本 健太,坂本 祥平,前田 元,増本 奉之,馬場 則男
    • Organizer
      日本顕微鏡学会第71回学術講演会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report
  • [Book] ライフサイエンス顕微鏡学ハンドブック、執筆:「2電顕トモグラフィー」馬場則男、馬場美鈴2018

    • Author(s)
      山科正平、高田邦昭
    • Total Pages
      344
    • Publisher
      朝倉書店
    • ISBN
      9784254310948
    • Related Report
      2017 Annual Research Report
  • [Remarks] 工学院大学 研究者情報データベース

    • URL

      http://www.kogakuin.ac.jp/research/index.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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