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electrochemical control of mas transfer and single cell analysis by multifunctional nanopipet

Research Project

Project/Area Number 15H03542
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nano/Microsystems
Research InstitutionTohoku University

Principal Investigator

Shiku Hitoshi  東北大学, 工学研究科, 教授 (10361164)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords走査型プローブ顕微鏡 / 液液界面 / 遺伝子発現解析 / 組織モデル / 胚性幹細胞 / 分化誘導 / イオンコンダクタンス / 電気化学 / 1細胞分析 / 遺伝子発現 / 分化 / 1細胞分析
Outline of Final Research Achievements

Recently, single-cell analysis has been developed to achieve highly sensitive and high-throughput system. Large-scale data analysis of gene expression profiles for actual tissue samples of colorectal cancer patients. However, in the present stage, expensive cell sorter is required to perform single-cell analysis of tissue samples. And the cell sorting process lose information about the original location of the single cells in the tissue samples. We have developed a single-cell analytical tool based on scanning probe microscopy to obtain high-resolution topographic images of tissue model. Sequentially, the SPM system allows to precisely collect a targeted single-cell or a part of cytosol sample from a single-cell for high-throughput gene expression analysis.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (45 results)

All 2018 2017 2016 2015 2014

All Journal Article (22 results) (of which Peer Reviewed: 22 results,  Open Access: 2 results,  Acknowledgement Compliant: 4 results) Presentation (23 results) (of which Int'l Joint Research: 8 results,  Invited: 15 results)

  • [Journal Article] Micropatterning of Nafion Membranes on an Electrode Array Using Photolithographic and Lift-off Techniques for Selective Electrochemical Detection and Signal Accumulation2018

    • Author(s)
      Terauchi Mayuko、Ino Kosuke、Kanno Yusuke、Imai Shunsuke、Shiku Hitoshi、Matsue Tomokazu
    • Journal Title

      Chemistry Letters

      Volume: 47 Issue: 2 Pages: 204-206

    • DOI

      10.1246/cl.171031

    • NAID

      130006337743

    • ISSN
      0366-7022, 1348-0715
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemicolor imaging of endogenous alkaline phosphatase and respiratory activities of mesenchymal stem cell aggregates in early-stage osteodifferentiation2018

    • Author(s)
      Ino Kosuke、Onodera Takehiro、Kanno Yusuke、Suda Atsushi、Kunikata Ryota、Matsue Tomokazu、Shiku Hitoshi
    • Journal Title

      Electrochimica Acta

      Volume: 268 Pages: 554-561

    • DOI

      10.1016/j.electacta.2018.02.094

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of oxygen consumption analysis with an on-chip electrochemical device and simulation2017

    • Author(s)
      Hiramoto Kaoru、Yasumi Masahiro、Ushio Hiroshi、Shunori Atsushi、Ino Kosuke、Shiku Hitoshi、Matsue Tomokazu
    • Journal Title

      Analytical Chemistry

      Volume: 89 Issue: 19 Pages: 10303-10310

    • DOI

      10.1021/acs.analchem.7b02074

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemicolor imaging using an LSI-Based device for multiplexed cell assays2017

    • Author(s)
      Yusuke Kanno, Kosuke Ino, Hiroya Abe, Chika Sakamoto, Takehiro Onodera, Kumi Y Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue
    • Journal Title

      Analytical Chemistry

      Volume: 89 Issue: 23 Pages: 12778-12786

    • DOI

      10.1021/acs.analchem.7b03042

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bioelectrochemical applications of microelectrode arrays in cell analysis and engineering2017

    • Author(s)
      Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue
    • Journal Title

      Current Opinion in Electrochemistry

      Volume: 5 Issue: 1 Pages: 146-151

    • DOI

      10.1016/j.coelec.2017.08.004

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 3D electrochemical and ion current imaging using scanning electrochemical-scanning ion conductance microscopy2017

    • Author(s)
      4)Takahashi, Y.; Ida, H.; Matsumae, Y.; Komaki, H.; Zhou, Y.; Kumatani, A.; Kanzaki, M.; Shiku, H.; Matsue, T.
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 19 Issue: 39 Pages: 26728-26733

    • DOI

      10.1039/c7cp05157c

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 超高解像度電気化学顕微鏡の創成と応用2017

    • Author(s)
      松岡涼, 青柳重夫, 松本尚志, 松平正昭, 高橋康史, 熊谷明近哉, 井田大貴, 棟方裕一, 飯田克彦, 珠玖 仁, 金村聖志, 末永智一
    • Journal Title

      Electrochemistry

      Volume: 85 Pages: 319-326

    • NAID

      130007342836

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical motion tracking of microorganisms using a large-scale integration (LSI)-based amperometric device2017

    • Author(s)
      Kosuke Ino, Yusuke Kanno, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 56 Issue: 24 Pages: 6818-6822

    • DOI

      10.1002/anie.201701541

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High speed scanning ion conductance microscopy for quantitative analysis of nanoscale dynamics of microvilli2017

    • Author(s)
      Hiroki Ida, Yasufumi Takahashi, Hitoshi Shiku,Tomokazu Matsue
    • Journal Title

      Analytical Chemistry

      Volume: 89 Issue: 11 Pages: 6015-6020

    • DOI

      10.1021/acs.analchem.7b00584

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Continuous collection and simultaneous detection of picoliter volume of nucleic acid samples using a mille-feuille probe2017

    • Author(s)
      Ito Hidenori、Tanaka Motoki、Zhou Yuanshu、Nashimoto Yuji、Takahashi Yasufumi、Ino Kosuke、Matsue Tomokazu、Shiku Hitoshi
    • Journal Title

      Anal. Bioanal. Chem.

      Volume: 409 Issue: 4 Pages: 961-969

    • DOI

      10.1007/s00216-016-0006-y

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell Sheet Fabrication Using RGD Peptide-Coupled Alginate Hydrogels Fabricated by an Electrodeposition Method.2017

    • Author(s)
      F. Ozawa, K. Ino,* Y. Takahashi, H. Shiku, T. Matsue*.
    • Journal Title

      Chem. Lett.

      Volume: 45 Issue: 4 Pages: 605-608

    • DOI

      10.1246/cl.170003

    • NAID

      130005549081

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Binary-number-based digital electrochemical detection using a single working electrode with multiple sensors2017

    • Author(s)
      K. Ino, Y. Kanno, Y. Yamada, H. Shiku, T. Matsue
    • Journal Title

      Electrochemistry Communications

      Volume: 77 Pages: 76-80

    • DOI

      10.1016/j.elecom.2017.02.016

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Scanning Probe Microscopy for Nanoscale Electrochemical Imaging2017

    • Author(s)
      Takahashi, Y., Kumatani, A.; Shiku, H.; Matsue, T.
    • Journal Title

      Analytical Chemistry

      Volume: 89 Issue: 1 Pages: 342-357

    • DOI

      10.1021/acs.analchem.6b04355

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] An approach to the research on ion and water properties in the interphase between the plasma membrane and bulk extracellular solution2017

    • Author(s)
      Hiroshi Hibino, Madoka Takai, Hidenori Noguchi, Seishiro Sawamura, Yasufumi Takahashi, Hideki Sakai, Hitoshi Shiku
    • Journal Title

      J. Physiol. Sci.

      Volume: 67 Issue: 4 Pages: 439-445

    • DOI

      10.1007/s12576-017-0530-3

    • NAID

      40021238302

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of mRNA Localization Using Double Barrel Scanning Ion Conductance Microscopy2016

    • Author(s)
      Nashimoto Yuji、Takahashi Yasufumi、Zhou Yuanshu、Ito Hidenori、Ida Hiroki、Ino Kosuke、Matsue Tomokazu、Shiku Hitoshi
    • Journal Title

      ACS Nano

      Volume: 10 Issue: 7 Pages: 6915-6922

    • DOI

      10.1021/acsnano.6b02753

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sequential Monitoring of Oxygen Consumption Rate of Mouse Embryoid Bodies in Glucose-Depleted Solution2016

    • Author(s)
      H. Shiku*, N. Aoki, T. Arai, Y. Zhou, K. Y. Inue, K. Ino, T. Matsue*.
    • Journal Title

      Electrochemistry

      Volume: 84 Issue: 5 Pages: 302-304

    • DOI

      10.5796/electrochemistry.84.302

    • NAID

      130005150418

    • ISSN
      1344-3542, 2186-2451
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Redox Cycling-based Electrochemical Reporter Gene Assay for Single Cells Using a Scanning Electrochemical Microscope-microwell System2016

    • Author(s)
      H. Ida, K. Ino, J. Suzuki, Y. Kanno, H. Shiku, T. Matsue*.
    • Journal Title

      Electrochemistry

      Volume: 84 Issue: 5 Pages: 308-311

    • DOI

      10.5796/electrochemistry.84.308

    • NAID

      130005150421

    • ISSN
      1344-3542, 2186-2451
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical Imaging for Single-cell Analysis of Cell Adhesion Using a Collagen-coated Large-scale Integration (LSI)-based Amperometric Device2016

    • Author(s)
      Abe H, Kanno Y, Ino K, Inoue KY, Suda A, Kunikata R, Matsudaira M, Shiku H, Matsue T
    • Journal Title

      Electrochemistry

      Volume: 84 Issue: 5 Pages: 364-367

    • DOI

      10.5796/electrochemistry.84.364

    • NAID

      130005150427

    • ISSN
      1344-3542, 2186-2451
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Electrochemical hydrogel lithography of calcium-alginate hydrogels for cell culture2016

    • Author(s)
      Fumisato Ozawa, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue
    • Journal Title

      Materials

      Volume: 9 Issue: 9 Pages: 744-744

    • DOI

      10.3390/ma9090744

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Localized gene expression analysis during sprouting angiogenesis in mouse embryoid bodies using a double barrel carbon probe2016

    • Author(s)
      H Ito, Y Nashimoto, Y Zhou, Y Takahashi, K Ino, H Shiku*, T Matsue*
    • Journal Title

      Anal Chem.

      Volume: 88 Issue: 9 Pages: 610-613

    • DOI

      10.1039/c5ra23454a

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Evaluation of senescence in indivicual MCF-7 spheroids based on electrochemical measurement of senescence-associated β-galactosidase activity2015

    • Author(s)
      Zhou Y, Ino K, Shiku H, Matsue T
    • Journal Title

      Electrochim. Acta

      Volume: 186 Pages: 449-454

    • DOI

      10.1016/j.electacta.2015.10.115

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Three-dimensional co-culture of C2C12/PC12 cells improves skeletal muscle tissue formation and function.2014

    • Author(s)
      Ostrovidov, S., Ahadian, S., Ramon-Azcon, J., Hosseini, V., Fujie, T., Prakash Parthiban, S., Shiku, H., Matsue, T., Kaji, H., Ramalingam, M., Bae, H., Khademhosseini, A.
    • Journal Title

      Journal of Tissue Engineering and Regenerative Medicine

      Volume: - Issue: 2 Pages: 582-595

    • DOI

      10.1002/term.1956

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] Characterization of multicellular spheroids and embryoid bodies by electrochemical imaging2018

    • Author(s)
      珠玖 仁
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 多機能電極プローブによる細胞塊の1細胞分析2018

    • Author(s)
      珠玖 仁
    • Organizer
      電気化学会第85回大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 多点電極デバイスによる三次元培養系試料の電気化学イメージング2018

    • Author(s)
      珠玖 仁
    • Organizer
      第8回生体界面研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Noninvasive electrochemical characterization of multicellular spheroids and embryobodies2018

    • Author(s)
      Hitoshi Shiku
    • Organizer
      9th international workshop on nanostructures & nanoelectronics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Oxygen Consumption of Individual Mammalian Embryos, Embryoid Bodies, and Multicellular Spheroids Probed by Scanning Electrochemical Microscopy.2017

    • Author(s)
      H. Shiku
    • Organizer
      The 19th Chongqing ART Conference (2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 多機能電極プローブによる生細胞・生体界面の局所分析2017

    • Author(s)
      珠玖 仁
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 走査型イオンコンダクタンス顕微鏡による細胞・生体界面のナノイメージング2017

    • Author(s)
      珠玖 仁
    • Organizer
      2017年真空学会・表面科学会合同公演会(第37回表面科学学術講演会、第58回真空に関する連合講演会)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 電気化学センシングに基づく生体機能評価2017

    • Author(s)
      珠玖 仁
    • Organizer
      みちのく分析科学シンポジウム2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Visualization of Nanoscale Neuron Surface Topography and Detection of Neurotransmitter Release Using Nano Electrochemical Microscopy.2016

    • Author(s)
      T. Yasufumi , S. Hitoshi, T. Matsue
    • Organizer
      PITTCON 2016
    • Place of Presentation
      Georgia World Congress Center, Atlanta, GA USA
    • Year and Date
      2016-03-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Electrochemical micro- and nano-analysis of embryos and embryonic stem cells,2016

    • Author(s)
      Hitoshi Shiku
    • Organizer
      Tohoku University's Chemistry Summer School 2016
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Scanning probe microscopy for high-resolution and multi-functional images2016

    • Author(s)
      Hitoshi Shiku
    • Organizer
      平成28年度化学系学協会東北大会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] センシング技術による細胞機能評価2016

    • Author(s)
      珠玖 仁
    • Organizer
      第39回 教師のための化学教育講座
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] mRNA analysis of living cells after capturing SICM topography of the living cell membrane2015

    • Author(s)
      H. Shiku, Y. Nashimoto, Y. Takahashi, H. Ito, Y. Zhou, K. Ino, T. Matsue
    • Organizer
      The 2015 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Honolulu, Hawaii
    • Year and Date
      2015-12-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation of senescence-associated β-galactosidase activity in single cell using scanning electrochemical microscope2015

    • Author(s)
      Y. Zhou, K. Ino, H. Shiku, T. Matsue
    • Organizer
      The 2015 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Honolulu, Hawaii
    • Year and Date
      2015-12-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Integrated nanopipette probes to obtain high-resolution bio-functional images2015

    • Author(s)
      H. Shiku
    • Organizer
      第25回日本MRS年次大会
    • Place of Presentation
      横浜情報文化センター, 横浜市
    • Year and Date
      2015-12-10
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 組織内におけるハイスループット局所遺伝子解析2015

    • Author(s)
      伊藤秀矩, 梨本裕司, 周縁殊, 田中元基, 高橋康史, 伊野浩介, 珠玖 仁, 末永智一
    • Organizer
      第5回CSJ化学フェスタ,
    • Place of Presentation
      タワーホール船堀, 東京
    • Year and Date
      2015-10-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] ガラスピペット内における細胞塊の培養とエクソソームの検出2015

    • Author(s)
      宮下紘介,井田大貴,梨本裕司,周縁殊,伊野浩介,高橋康史,珠玖 仁,末永智一
    • Organizer
      2015年電気化学秋季大会
    • Place of Presentation
      埼玉工業大学(埼玉県, 深谷市)
    • Year and Date
      2015-09-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] 走査型電気化学顕微鏡を用いた細胞老化評価法の開発2015

    • Author(s)
      周 縁殊, 伊野 浩介, 珠玖 仁, 末永 智一
    • Organizer
      2015年電気化学秋季大会
    • Place of Presentation
      埼玉工業大学(埼玉県, 深谷市)
    • Year and Date
      2015-09-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] 電気化学計測に基づく受精卵および細胞塊の機能評価装置の開発2015

    • Author(s)
      珠玖 仁, 青柳重夫
    • Organizer
      日本分析化学会 第64年会
    • Place of Presentation
      九州大学伊都キャンパス(福岡県, 福岡市)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Multi-functional Nanopipette Probe for Single-cell Analysis of Tissue Models2015

    • Author(s)
      Hitoshi Shiku, Yuji Nashimoto, Yuanshu Zhou, Yasufumi Takahashi, Kosuke Ino, Tomokazu Matsue
    • Organizer
      日本分析化学会 第64年会,第2回アジア分析科学シンポジウム
    • Place of Presentation
      九州大学伊都キャンパス(福岡県, 福岡市)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] High throughput Electrode Probe Techniques for Local Gene Expression Analysis of Tissue Model2015

    • Author(s)
      H. Ito, Y. Nashimoto, Y. Takahashi, K. Ino, H. Shiku, T. Matsue
    • Organizer
      The 10th International Symposium on Chemical-Environmental-Biomedical Technology-2015(isCEBT2015)
    • Place of Presentation
      Hsinchu, Taiwan
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 胚様体における多項目分析および単一細胞レベルの遺伝子発現解析ツールの開発2015

    • Author(s)
      珠玖 仁,梨本裕司,周 縁殊,高橋康史,伊野浩介,井上(安田)久美,末永智一
    • Organizer
      日本動物細胞工学会大会(JAACT2015)
    • Place of Presentation
      東北大学片平さくらホール(宮城県, 仙台市)
    • Year and Date
      2015-07-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Scanning Electrode Probe Technique for Analyzing Tissue Models at Single Cell Level2015

    • Author(s)
      Hitoshi Shiku, Yuji Nashimoto, Hidenori Ito, Yuasufumi Takahashi, Yuanshu Zhou, Kumi Y. Inoue, Kosuke Ino, Tomokazu Matsue
    • Organizer
      The XXIII International Symposium on Bioelectrochemistry and Bioenergetics
    • Place of Presentation
      Malmoe, Sweden
    • Year and Date
      2015-06-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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