electrochemical control of mas transfer and single cell analysis by multifunctional nanopipet
| Project/Area Number |
15H03542
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nano/Microsystems
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| Research Institution | Tohoku University |
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Principal Investigator |
Shiku Hitoshi 東北大学, 工学研究科, 教授 (10361164)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | 走査型プローブ顕微鏡 / 液液界面 / 遺伝子発現解析 / 組織モデル / 胚性幹細胞 / 分化誘導 / イオンコンダクタンス / 電気化学 / １細胞分析 / 遺伝子発現 / 分化 / 1細胞分析 |
|---|
| Outline of Final Research Achievements |
Recently, single-cell analysis has been developed to achieve highly sensitive and high-throughput system. Large-scale data analysis of gene expression profiles for actual tissue samples of colorectal cancer patients. However, in the present stage, expensive cell sorter is required to perform single-cell analysis of tissue samples. And the cell sorting process lose information about the original location of the single cells in the tissue samples. We have developed a single-cell analytical tool based on scanning probe microscopy to obtain high-resolution topographic images of tissue model. Sequentially, the SPM system allows to precisely collect a targeted single-cell or a part of cytosol sample from a single-cell for high-throughput gene expression analysis.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Electrochemicolor imaging using an LSI-Based device for multiplexed cell assays2017
Author(s)
Yusuke Kanno, Kosuke Ino, Hiroya Abe, Chika Sakamoto, Takehiro Onodera, Kumi Y Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue
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Journal Title
Analytical Chemistry
Volume: 89
Issue: 23
Pages: 12778-12786
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] 超高解像度電気化学顕微鏡の創成と応用2017
Author(s)
松岡涼, 青柳重夫, 松本尚志, 松平正昭, 高橋康史, 熊谷明近哉, 井田大貴, 棟方裕一, 飯田克彦, 珠玖 仁, 金村聖志, 末永智一
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Journal Title
Electrochemistry
Volume: 85
Pages: 319-326
NAID
Related Report
Peer Reviewed
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[Journal Article] Three-dimensional co-culture of C2C12/PC12 cells improves skeletal muscle tissue formation and function.2014
Author(s)
Ostrovidov, S., Ahadian, S., Ramon-Azcon, J., Hosseini, V., Fujie, T., Prakash Parthiban, S., Shiku, H., Matsue, T., Kaji, H., Ramalingam, M., Bae, H., Khademhosseini, A.
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Journal Title
Journal of Tissue Engineering and Regenerative Medicine
Volume: -
Issue: 2
Pages: 582-595
DOI
Related Report
Peer Reviewed
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