| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
Recently, single-cell analysis has been developed to achieve highly sensitive and high-throughput system. Large-scale data analysis of gene expression profiles for actual tissue samples of colorectal cancer patients. However, in the present stage, expensive cell sorter is required to perform single-cell analysis of tissue samples. And the cell sorting process lose information about the original location of the single cells in the tissue samples. We have developed a single-cell analytical tool based on scanning probe microscopy to obtain high-resolution topographic images of tissue model. Sequentially, the SPM system allows to precisely collect a targeted single-cell or a part of cytosol sample from a single-cell for high-throughput gene expression analysis.
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