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Multimodal Digital Holographic Microscopy for Simultaneous Measurement of 3D Fluorescence and 3D Phase

Research Project

Project/Area Number 15H03580
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Optical engineering, Photon science
Research InstitutionKobe University

Principal Investigator

MATOBA OSAMU  神戸大学, システム情報学研究科, 教授 (20282593)

Co-Investigator(Renkei-kenkyūsha) MIZUKAMI Tamio  長浜バイオ大学, バイオサイエンス学部, 教授 (80367896)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywordsマルチモーダルディジタルホログラフィック顕微鏡 / 蛍光 / 位相 / マルチモーダル光学顕微鏡 / 蛍光ディジタルホログラフィック顕微鏡 / 位相イメージング / ディジタルホログラフィー / マルチモーダルイメージング / ディジタルホログラフィ / インコヒーレントディジタルホログラフィ / 蛍光イメージング / マルチモーダル / 顕微鏡
Outline of Final Research Achievements

In this research, we proposed a multimodal digital holographic microscopy (DHM) that can realize simultaneous measurement of three-dimensional (3D) fluorescence and 3D phase imaging. We fabricated a multimodal DHM that can obtain simultaneously two-dimensional fluorescence distribution and 3D phase distribution. The effectiveness of the proposed DHM was confirmed by the experimental results of plant cells and mouse's brain cells. Furthermore, for the development of single shot measurement of 3D fluorescence distribution, we proposed the common-path off-axis incoherent DHM and its feasibility was confirmed by the experiment.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (39 results)

All 2017 2016 2015

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Acknowledgement Compliant: 3 results) Presentation (33 results) (of which Int'l Joint Research: 24 results,  Invited: 15 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Image recovery from defocused 2D fluorescent images in multimodal digital holographic microscopy2017

    • Author(s)
      Quan Xiangyu、Matoba Osamu、Awatsuji Yasuhiro
    • Journal Title

      Optics Letters

      Volume: 42 Issue: 9 Pages: 1796-1796

    • DOI

      10.1364/ol.42.001796

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multimodal Imaging Based on Digital Holography2017

    • Author(s)
      Osamu Matoba, Xiangyu Quan, Peng Xia, Yasuhiro Awatsuji, and Takanori Nomura
    • Journal Title

      Proc. IEEE

      Volume: 105 Issue: 5 Pages: 906-923

    • DOI

      10.1109/jproc.2017.2656148

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Single-shot incoherent digital holography using a dual-focusing lens with diffraction gratings2016

    • Author(s)
      Xiangyu Quan, Osamu Matoba, Yasuhiro Awatsuji
    • Journal Title

      Optics Letters

      Volume: 42 Issue: 3 Pages: 383-386

    • DOI

      10.1364/ol.42.000383

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Multidimensional measurement by hybrid digital holographic microscopy for biological applications2015

    • Author(s)
      Osamu Matoba, Xiangyu Quan, Peng Xia, Yasuhiro Awatsuji
    • Journal Title

      Asian Journal of Physics

      Volume: 24 Pages: 1467-1471

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Presentation] Common-path incoherent digital holography2017

    • Author(s)
      Osamu Matoba, Xiangyu Quan, and Yasuhiro Awatsuji
    • Organizer
      SPIE Commercial + Scientific Sensing and Imaging
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Off-axis incoherent digital holographic microscopy2017

    • Author(s)
      Xiangyu Quan, Osamu Matoba, and Yasuhiro Awatsuji
    • Organizer
      SPIE Commercial + Scientific Sensing and Imaging
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of common-path incoherent digital holography using dual-focusing lens with diffraction gratings2017

    • Author(s)
      X. Quan, A. Moriyama, K. Nitta, O. Matoba, Y. Awatsuji
    • Organizer
      Biomedical Imaging and Sensing Conference (BSIC'17)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Recovery of fluorescent bead image by phase compensation method based on the defocusing distance2017

    • Author(s)
      X. Quan, O. Matoba, and Y. Awatsuji
    • Organizer
      Digital Holography and Three-Dimensional Imaging (DH) 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 3D fluorescence imaging based on digital holography2017

    • Author(s)
      O. Matoba, X. Quan, and Y. Awatsuji
    • Organizer
      16th Workshop on Information Optics (WIO2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Simultaneous imaging of 3D phase and 3D fluorescence for biological application2017

    • Author(s)
      X. Quan, O. Matoba, and Y. Awatsuji
    • Organizer
      16th Workshop on Information Optics (WIO2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of Off-axis Incoherent Digital Holography based on Dual Lenses with Diffraction Gratings2017

    • Author(s)
      A. Moriyama, X. Quan, K. Nitta, O. Matoba
    • Organizer
      The 24th Congress of the International Commission for Optics (ICO-24)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A simple deblurring method by phase correction in the Fourier domain of out-of-focus images2017

    • Author(s)
      X. Quan, O. Matoba, Y. Awatsuji
    • Organizer
      The 24th Congress of the International Commission for Optics (ICO-24)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multimodality of phase and fluorescence in digital holography2017

    • Author(s)
      O. Matoba, X. Quan, and Y. Awatsuji
    • Organizer
      The 3rd International Conference on Photonics Solutions (ICPS2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fluorescence imaging by common-path off-axis digital holography2017

    • Author(s)
      O. Matoba, X. Quan, and Y. Awatsuji
    • Organizer
      International Workshop on Holography and Related Technologies 2017 (IWH2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 蛍光画像のフーリエ変換面での位相補正方法2017

    • Author(s)
      全 香玉, 仁田 功一, 的場 修, 粟辻 安浩
    • Organizer
      日本光学会年次学術講演会(Optics & Photonics Japan 2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] ディジタルホログラフィック顕微鏡によるマルチモーダルイメージングシンポジウム2017

    • Author(s)
      的場修,全香玉,粟辻安浩
    • Organizer
      第64回応用物理学会春季学術講演会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Experimental verification of phase retrieval of microbeads in high-speed phase imaging using digital holography2016

    • Author(s)
      Osamu Matoba, Peng Xia, Xiangyu Quan, Naoya Nagahama, Shunsuke Tanimoto, Kouchi Nitta, and Yasuhiro Awatsuji
    • Organizer
      SPIE DSS, Sensing Technology + Applications
    • Place of Presentation
      ボルチモダ(アメリカ)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Application of a dual-excitation multi-modal digital holographic microscope to biological imaging2016

    • Author(s)
      X. Quan, P. Xia, Y. Awatsuji, and O. Matoba
    • Organizer
      Collaborative Conference on 3D & Materials Research 2016 (CC3DMR 2016)
    • Place of Presentation
      ソウル(韓国)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multi-modal digital holography for live cell imaging2016

    • Author(s)
      O. Matoba, X. Quan, Y. Tamada, and Y. Awatsuji
    • Organizer
      15th Workshop on Information Optics (WIO2016)
    • Place of Presentation
      バルセロナ(スペイン)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multi-modal digital holographic microscopy and demonstration on dual-excitation fluorescence2016

    • Author(s)
      X. Quan, P. Xia, K. Nitta, O. Matoba, and Y. Awatsuji
    • Organizer
      Digital Holography and Three-Dimensional Imaging (DH) 2016
    • Place of Presentation
      ハイデルベルグ(ドイツ)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multi-modal Digital Holographic Microscope with Dualwavelength Excitation and Dual-wavelength Phase Imaging2016

    • Author(s)
      X. Quan, O. Matoba, K. Nitta, Y. Awatsuji, Y. Takizawa
    • Organizer
      第77回応用物理学会秋季学術講演会
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Digital holographic imaging and sensing for biology and sound wave measurement2016

    • Author(s)
      O. Matoba, X. Quan, and Y. Awatsuji
    • Organizer
      Photonics Asia 2016
    • Place of Presentation
      北京(中国)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] New configuration of Incoherent digital holography by dual focal lenses with grating2016

    • Author(s)
      X. Quan, O. Matoba, Y. Awatsuji
    • Organizer
      OSA-OSJ Joint Symposia in Optics & Photonics Japan 2016
    • Place of Presentation
      筑波大学東京キャンパス文京校舎(東京都文京区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Image recovery of fluprescence light from phase hologram in multi-modal digital holographic microscopy2016

    • Author(s)
      X. Quan, O. Matoba, Y. Awatsuji
    • Organizer
      OSA-OSJ Joint Symposia in Optics & Photonics Japan 2016
    • Place of Presentation
      筑波大学東京キャンパス文京校舎(東京都文京区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] マルチモーダルディジタルホログラフィック顕微鏡と植物細胞イメージング2016

    • Author(s)
      的場 修, 全 香玉,粟辻 安浩
    • Organizer
      Optics & Photonics Japan 2016
    • Place of Presentation
      筑波大学東京キャンパス文京校舎(東京都文京区)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Evaluations on Reconstruction Distance in Incoherent Digital Holography with a Diffraction Grating2016

    • Author(s)
      X. Quan, O. Matoba, Y. Awatsuji
    • Organizer
      The 6th Japan-Korea Workshop on Digital Holography and Information Photonics (DHIP2016)
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multimodal imaging of 3D phase and 2D fluorescence distributions using digital holographic microscopy2016

    • Author(s)
      O. Matoba, X. Quan, Y. Awatsuji
    • Organizer
      The 6th Japan-Korea Workshop on Digital Holography and Information Photonics (DHIP2016)
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 3D Sensing by Digital Holography2015

    • Author(s)
      O. Matoba, X. Quan, H. Inokuchi, P. Xia, Y. Awatsuji
    • Organizer
      The 15th International Conference on Computers, Communications and Systems (ICCCS2015)
    • Place of Presentation
      Daegu University(Daegu, Korea)
    • Year and Date
      2015-11-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ディジタルホログラフィック顕微鏡における高速移動位相物体の位相再構成像の改善2015

    • Author(s)
      夏 鵬,仁田 功一,的場 修,粟辻 安浩
    • Organizer
      Optics & Photonics Japan 2015 (OPJ2015)
    • Place of Presentation
      筑波大学東京キャンパス(東京都,文京区)
    • Year and Date
      2015-10-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生体細胞の3次元位相像と2次元蛍光像の同時動画計測実験2015

    • Author(s)
      全 香玉, 夏 鵬, 仁田 功一,的場 修, 粟辻 安浩
    • Organizer
      Optics & Photonics Japan 2015 (OPJ2015)
    • Place of Presentation
      筑波大学東京キャンパス(東京都,文京区)
    • Year and Date
      2015-10-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Phase and fluorescence imaging by digital holographic microscopy2015

    • Author(s)
      O. Matoba, X. Quan, P. Xia, Y. Awatsuji
    • Organizer
      The 5th Korea-Japan Workshop on Digital Holography and Information Photonics (DHIP2015)
    • Place of Presentation
      KIST(Gangneung, Korea)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ディジタルホログラフィック顕微鏡による高速3次元位相物体計測2015

    • Author(s)
      土谷和弘,仁田功一,的場修
    • Organizer
      3次元画像コンファレンス 2015
    • Place of Presentation
      海洋研究開発機構(神奈川県・横浜市)
    • Year and Date
      2015-07-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] A hybrid digital holographic microscopy for biological applications2015

    • Author(s)
      O. Matoba, X. Quan, Y. Awatuji
    • Organizer
      The 2nd International Conference on Photonics Solutions (ICPS2015)
    • Place of Presentation
      Dusit Thani Hotel(Hua Hin, Thailand)
    • Year and Date
      2015-07-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A hybrid digital holographic microscopy2015

    • Author(s)
      O. Matoba
    • Organizer
      14th workshop on information optics (WIO2015)
    • Place of Presentation
      京都ガーデンパレス(京都府・京都市)
    • Year and Date
      2015-06-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Phase measurement using high-speed movable stage by digital holography under CW laser illumination2015

    • Author(s)
      P. Xia, X. Quan, K. Nitta, O. Matoba, Y. Awatsuji
    • Organizer
      14th workshop on information optics (WIO2015)
    • Place of Presentation
      京都ガーデンパレス(京都府・京都市)
    • Year and Date
      2015-06-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Hybrid digital holographic microscope for simultaneous measurement of 3D phase and 3D fluorescence distributions and its signal processing2015

    • Author(s)
      X. Quan, P. Xia, K. Nitta, O. Matoba, Y. Awatsuji
    • Organizer
      14th workshop on information optics (WIO2015)
    • Place of Presentation
      京都ガーデンパレス(京都府・京都市)
    • Year and Date
      2015-06-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Integrated fluorescence and phase-contrast digital holographic microscopy for live cell imaging2015

    • Author(s)
      X. Quan, K. Nitta, O. Matoba, Y. Awatsuji
    • Organizer
      SPIE DSS, Sensing Technology + Applications
    • Place of Presentation
      Baltimore (USA)
    • Year and Date
      2015-04-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] ディジタルホログラフィック顕微鏡2017

    • Inventor(s)
      的場修
    • Industrial Property Rights Holder
      国立大学法人神戸大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] ディジタルホログラフィック顕微鏡2016

    • Inventor(s)
      的場 修
    • Industrial Property Rights Holder
      国立大学法人神戸大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-200457
    • Filing Date
      2016-10-11
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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