Expression of an artificial action potential and its application to screening devices for drug side effects
| Project/Area Number |
15H03822
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Analytical chemistry
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
山本 英明 東北大学, 学際科学フロンティア研究所, 助教 (10552036)
馬 騰 東北大学, 材料科学高等研究所, 助教 (10734543)
但木 大介 東北大学, 電気通信研究所, 助教 (30794226)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
NIWANO MICHIO 東北福祉大学, 感性福祉研究所, 教授 (20134075)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
|
|---|
| Keywords | マイクロ・ナノデバイス / ナノバイオ / イオンチャネル / シリコンチップ / 活動電位 / 脂質二分子膜 |
|---|
| Outline of Final Research Achievements |
In recent years, the risk evaluation of potential drug side effects to the heart is indispensable in drug discovery, and it is highly desirable to construct an artificial system for this evaluation. In this study, we tried to develop a screening platform for potential drug side effects based on artificially formed bilayer lipid membranes (BLMs) that can express action potentials. However, we faced an issue of a low efficiency in incorporation of ion-channels that are necessary for generation of action potentials. To address this issue, we investigated the process of ion channel incorporation and found that application of appropriate centrifugal forces during the incorporation process lead to a dramatic enhancement of the incorporation probability from ca. 6% to ca. 70%. We also investigated development of high-throughput assay systems for the ion channels and succeeded in simultaneous recordings of hERG channel activities in BLM systems.
|
Report
(4 results)
Research Products
(121 results)
-
-
-
[Journal Article] Amphiphobic Septa Enhance the Mechanical Stability of Free-Standing Bilayer Lipid Membranes2018
Author(s)
Daichi Yamaura, Daisuke Tadaki, Shun Araki, Miyu Yoshida, Kohei Arata, Takeshi Ohori, Ken-ichi Ishibashi, Miki Kato, Teng Ma, Ryusuke Miyata, Hideaki Yamamoto, Ryugo Tero, Masao Sakuraba, Toshio Ogino, Michio Niwano, Ayumi Hirano-Iwata
-
Journal Title
Langmuir
Volume: 印刷中
Issue: 19
Pages: 5615-5622
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels.2017
Author(s)
Daisuke Tadaki, Daichi Yamaura, Shun Araki, Miyu Yoshida, Kohei Arata, Takeshi Ohori, Ken-ichi Ishibashi, Miki Kato, Teng Ma, Ryusuke Miyata, Yuzuru Tozawa, Hideaki Yamamoto, Michio Niwano, and Ayumi Hirano-Iwata
-
Journal Title
Scientific Report
Volume: 7
Issue: 1
Pages: 17736-17736
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] Astrocytes with previous chronic exposure to amyloid beta-peptide fragment 1–40 suppress excitatory synaptic transmission.2017
Author(s)
Kawano H, Oyabu K, Yamamoto H, Eto K, Adaniya Y, Kubota K, Watanabe T, Hirano-Iwata A, Nabekura J, Katsurabayasi S, Iwasaki K.
-
Journal Title
J Neurochemistry
Volume: 143
Issue: 6
Pages: 624-634
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Molecular doping of regioregular poly(3-hexylthiophene) layers by 2,3,5,6-tetrafluoro-7,7,8,8-tetracyanoquinodimethane investigated by infrared spectroscopy and electrical measurements2015
Author(s)
Daisuke Tadaki, Teng Ma, Jinyu Zhang, Shohei Iino, Ayumi Hirano-Iwata, Yasuo Kimura, and Michio Niwano
-
Journal Title
Jpn. J. Appl. Phys
Volume: 54
Issue: 9
Pages: 091602-091602
DOI
NAID
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] エクソソーム-基板間の相互作用を利用した特徴抽出2016
Author(s)
小川 裕太, 伊藤 和希, 横田 圭司, 松村 幸子, 南澤 宝美后, 菅 加奈子, 芝 清隆, 木村 康男, 平野 愛弓, 荻野 俊郎
Organizer
第77回応用物理学会秋季学術講演
Place of Presentation
朱鷺メッセ，新潟
Year and Date
2016-09-13
Related Report
-
-
-
-
[Presentation] Fabrication method of silicon chips with micro-apertures for formation of artificial bilayer lipid membranes2016
Author(s)
D. Tadaki, A. Hirano-Iwata, K. Ishibashi, S. Araki, M. Yoshida, K. Arata, T. Ohori, H. Yamamoto, M. Niwano
Organizer
20th International Vacuum Congress (IVC-20)
Place of Presentation
BEXCO (Busan Exhibition Convention Center), Busan, Korea
Year and Date
2016-08-21
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 固体基板への固定による生体物質の評価2016
Author(s)
荻野俊郎, 伊藤和希, 横田圭司, 小川裕太, 松村幸子, 南澤宝美后, 菅加奈子, 芝清隆, 木村康男, 平野愛弓
Organizer
電子情報通信学会 電子デバイス(ED）研究会
Place of Presentation
山形大学，米沢
Year and Date
2016-04-21
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
