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Correlation between property and fluctuation of the lipid membranes

Research Project

Project/Area Number 15H03824
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionChiba University

Principal Investigator

Fujinami Masanori  千葉大学, 大学院工学研究院, 教授 (50311436)

Co-Investigator(Kenkyū-buntansha) 豊田 太郎  東京大学, 総合文化研究科, その他 (80422377)
Co-Investigator(Renkei-kenkyūsha) NOMOTO Tomonori  千葉大学, 大学院工学研究院, 助教 (00510520)
Research Collaborator CHIARI Luca  千葉大学, 大学院工学研究院, 特任助教
WATAHIKI Yasuhito  千葉大学, 大学院融合理工学府, 大学院生
TAKAHASHI Masahiro  千葉大学, 大学院工学研究科, 大学院生
FUJII Takuya  千葉大学, 大学院工学研究科, 大学院生
KARASAWA Yuichiro  千葉大学, 大学院工学研究科, 大学院生
IKO Satoshi  千葉大学, 大学院工学研究科, 大学院生
TAKEI Tomohiko  千葉大学, 大学院工学研究科, 大学院生
UEMOTO Shoko  千葉大学, 大学院工学研究科, 大学院生
GOTO Kazuma  千葉大学, 大学院工学研究科, 大学院生
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Keywords分析科学 / 生体物理 / 生体分子 / 生体膜 / 表面・界面物性 / 界面活性分子 / 生物物理
Outline of Final Research Achievements

In the current study, the membrane tension and the diffusion coefficients of free-standing black lipid membranes (BLMs) were observed by systematic control of the cholesterol concentration and the osmotic pressure with the laser induced surface deformation (LISD) and fluorescence recovery after photobleaching (FRAP) techniques. When the osmotic pressure was raised, both the membrane tension and diffusion coefficients increased as well. On the other hand, when the cholesterol concentration was raised, the membrane tension increased whereas the diffusion coefficient decreased. The results are not only important as the quantitative evaluations about relations between membrane tension and fluidity, but also important as the fundamental properties of lipid bilayers with natural fluctuations and perturbative deformation that were unavailable before.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (19 results)

All 2018 2017 2016 2015 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (12 results) (of which Int'l Joint Research: 6 results) Remarks (2 results)

  • [Journal Article] Motion modes of two self-propelled camphor boats on the surface of a surfactant-containing solution2018

    • Author(s)
      Yuichiro Karasawa, Tomonori Nomoto, Luca Chiari, Taro Toyota, Masanori Fujinami
    • Journal Title

      Journal of Colloid and Interface Science

      Volume: 511 Pages: 184-192

    • DOI

      10.1016/j.jcis.2017.09.099

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assemblies of molecular aggregates in the blebbing motion of an oil droplet on an aqueous solution containing surfactant2017

    • Author(s)
      Shoko Uemoto, Taro Toyota, Luca Chiari, Tomonori Nomoto, Masanori Fujinami
    • Journal Title

      Colloids and Surfaces A

      Volume: 529 Pages: 373-379

    • DOI

      10.1016/j.colsurfa.2017.06.016

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurement of membrane tension of free standing lipid bilayers via laser-induced surface deformation spectroscopy2015

    • Author(s)
      Tomohiko Takei, Tatsuya Yaguchi, Takuya Fujii, Tomonori Nomoto, Taro Toyota, Masanori Fujinami
    • Journal Title

      Soft Matter

      Volume: 11 Issue: 44 Pages: 8641-8647

    • DOI

      10.1039/c5sm01264c

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effects of halide ions on the acceptor phase in spontaneous chemical oscillations in donor/membrane/acceptor systems2015

    • Author(s)
      Kazuma Goto, Tomonori Nomoto, Taro Toyota, Masanori Fujinami
    • Journal Title

      Journal of Colloid and Interface Science

      Volume: 462 Pages: 351-358

    • DOI

      10.1016/j.jcis.2015.10.013

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Changes in Interfacial Tension of a Lipid Membrane Formed at the Water/Chloroform Interface upon DNA Complex Formation2015

    • Author(s)
      Masashi Ohno, Taro Toyota, Tomonori Nomoto, Masanori Fujinami
    • Journal Title

      Analytical Sciences

      Volume: 31 Issue: 10 Pages: 979-986

    • DOI

      10.2116/analsci.31.979

    • NAID

      130005102882

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 液液界面におけるオレイン酸ナトリウム自走運動機構2017

    • Author(s)
      綿引靖人,Chiari Luca,豊田太郎,野本知理,藤浪眞紀
    • Organizer
      第77回分析化学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] レーザー誘起界面変形分光法による脂質二分子膜膜張力の膜組成および環境依存性2016

    • Author(s)
      藤井拓也,武井智彦,髙橋昌裕,野本知理,豊田太郎,藤浪眞紀
    • Organizer
      日本分析化学会 第65回年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 三相系化学振動現象における油相電解質の役割2016

    • Author(s)
      伊香智史,野本知理,豊田太郎,藤浪眞紀
    • Organizer
      日本分析化学会 第65回年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 水溶液表面での樟脳船集団運動の駆動力2016

    • Author(s)
      唐澤悠一郎,豊田太郎,野本知理,藤浪眞紀
    • Organizer
      日本分析化学会 第65回年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] Self-propelled motion of oil droplet and camphor boat on an aqueous phase2016

    • Author(s)
      Masanori Fujinami
    • Organizer
      The First International Conference on Mutiscale Applications of Surface Tension
    • Place of Presentation
      Brussels, Belgium
    • Year and Date
      2016-09-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of interfacial tension of spontaneous chemical oscillation in donor/membrane/acceptor systems2016

    • Author(s)
      Satoshi Iko, Kazuma Goto, Tomonori Nomoto, Taro Toyota , Masanori Fujinami
    • Organizer
      The First International Conference on Mutiscale Applications of Surface Tension
    • Place of Presentation
      Brussels, Belgium
    • Year and Date
      2016-09-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Membrane tension of free standing lipid bilayers by laser-induced surface deformation spectroscopy2016

    • Author(s)
      Masahiro Takahashi, Takuya Fujii, Tomohiko Takei, Tomonori Nomoto, Taro Toyota, Masanori
    • Organizer
      The First International Conference on Mutiscale Applications of Surface Tension
    • Place of Presentation
      Brussels, Belgium
    • Year and Date
      2016-09-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Non-invasive Surface Tension Measurement by Quasi-Elastic Laser Scattering Method: Mechanism of Spontaneous Motion of the Oil Droplet on an Aqueous Phase2015

    • Author(s)
      Masanori Fujinami
    • Organizer
      29th Conference of the European Colloid & Interface Society
    • Place of Presentation
      Bordeaux, France
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Surface Tension Distribution around a Camphor Boat Moving Spontaneously on a Water Surface2015

    • Author(s)
      Yuichiro Karasawa, Tomonori Nomoto, Taro Toyota, Masanori Fujinami
    • Organizer
      29th Conference of the European Colloid & Interface Society
    • Place of Presentation
      Bordeaux, France
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Spontaneous Chemical Oscillation Mechanism in water/oil/water system: Effect of chemical species in the acceptor phase2015

    • Author(s)
      Satoshi Iko, Kazuma Goto, Tomonori Nomoto, Taro Toyota, Masanori Fujinami
    • Organizer
      29th Conference of the European Colloid & Interface Society
    • Place of Presentation
      Bordeaux, France
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 油滴人工アメーバ運動における会合体挙動2015

    • Author(s)
      上本頌子,野本知理,豊田太郎,藤浪真紀
    • Organizer
      日本分析化学会第75回分析化学討論会
    • Place of Presentation
      山梨大学
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 水・油・水三相系化学振動現象における受容相液性依存性2015

    • Author(s)
      伊香智史,後藤和馬,野本知理,豊田太郎,藤浪真紀
    • Organizer
      日本分析化学会第75回分析化学討論会
    • Place of Presentation
      山梨大学
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Remarks]

    • URL

      http://chem.tf.chiba-u.jp/gacb11/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 千葉大学大学院工学研究科共生応用化学専攻計測化学研究室

    • URL

      http://chem.tf.chiba-u.jp/gacb11/index.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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