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Development of gel-pump microchip analysis system

Research Project

Project/Area Number 15H03827
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionOsaka University

Principal Investigator

Yamaguchi Yoshinori  大阪大学, 工学研究科, 招へい教授 (20386634)

Co-Investigator(Kenkyū-buntansha) 原 雄介  国立研究開発法人産業技術総合研究所, 材料・化学領域, 研究グループ長 (90452135)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Keywords分析化学 / ナノ・マイクロデバイス / 自励振動ゲル / クロマトグラフィー / マイクロチップ / キャピラリー電気泳動 / ワンプッシュ分析 / ゲルアクチュエータ / 分離分析 / 合成化学
Outline of Final Research Achievements

In chemical analysis including the synthesis and separation, the system for generating a solution flow, which is called “pump system” is required. Thus, the pump system is required to be miniaturized to adapt to the microchip system. To miniaturize the physical or mechanical pump, it is strong disadvantages that conventional mechanical pump is need to be architected by moving parts. And the source of the reactor which generate the force of pump should also be miniaturized to integrate in microfluidic system. Therefore, the lack of cheap and easy-to-integrate micro-pump including the power source was the fetal limitation for microfluidic system. In this study, we adopted the self-oscillating gel (BZ-gel) embedded into the microfluidic channel as the power source of the micro-pump. The self-oscillation of BZ gel generated sufficient physical forces to flow the solution in microchannel. We, here, proved that the simple oscillating BZ gel works for micro-pump for microfluidic system.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (20 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (12 results) (of which Int'l Joint Research: 12 results,  Peer Reviewed: 12 results,  Acknowledgement Compliant: 3 results) Presentation (7 results) (of which Int'l Joint Research: 4 results,  Invited: 7 results)

  • [Int'l Joint Research] 華東理工大学/上海理工大学(中国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Miniaturized gel electrophoresis system for fast separation of nucleic acids2018

    • Author(s)
      Li Zhenqing、Huang Jiaxin、Yang Bo、You Qingxiang、Sekine Shinichi、Zhang Dawei、Yamaguchi Yoshinori
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 254 Pages: 153-158

    • DOI

      10.1016/j.snb.2017.07.064

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Alignment and counting of mitochondria based on capillary electrophoresis2018

    • Author(s)
      Li Zhenqing、Yang Bo、Sekine Shinichi、Zhuang Songlin、Zhang Dawei、Yamaguchi Yoshinori
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 265 Pages: 110-114

    • DOI

      10.1016/j.snb.2018.03.032

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Precise and simultaneous enumeration of multiplex pathogens using multiplex polymer chain reaction coupled with a novel quantitative capillary electrophoresis2018

    • Author(s)
      Liu Chenchen、Yamaguchi Yoshinori、Sekine Shinichi、Ni Yi、Dou Xiaoming
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 258 Pages: 263-269

    • DOI

      10.1016/j.snb.2017.11.106

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Factors affecting the separation performance of proteins in capillary electrophoresis2018

    • Author(s)
      Zhu Yueping、Li Zhenqing、Wang Ping、Shen Lisong、Zhang Dawei、Yamaguchi Yoshinori
    • Journal Title

      Journal of Chromatography B

      Volume: 1083 Pages: 63-67

    • DOI

      10.1016/j.jchromb.2018.03.005

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Quantitative Detection of Ethanol/Acetone in Complex Solutions Using Raman Spectroscopy Based on Headspace Gas Analysis2017

    • Author(s)
      Zhao Yubin、Yamaguchi Yoshinori、Liu Chenchen、Sekine Shinichi、Dou Xiaoming
    • Journal Title

      Applied Spectroscopy

      Volume: 72 Issue: 2 Pages: 280-287

    • DOI

      10.1177/0003702817738010

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Real-time Tracking of DNA Fragment Separation by Smartphone2017

    • Author(s)
      Tao Chunxian、Yang Bo、Li Zhenqing、Zhang Dawei、Yamaguchi Yoshinori
    • Journal Title

      Journal of Visualized Experiments

      Volume: 124 Issue: 124

    • DOI

      10.3791/55926

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Non-invasive Video Image-based Analysis Method Coupled to Field Potential Recording for Evaluation of the Drug-induced Effect in Cardiac Tissue2016

    • Author(s)
      Wilfred V. Espulger, Masato Saito, Jong-Kook LEE, Yoshinori Yamaguchi, Eiichi Tamiya
    • Journal Title

      Electrochemistry

      Volume: 84 Issue: 5 Pages: 283-289

    • DOI

      10.5796/electrochemistry.84.283

    • NAID

      130005150436

    • ISSN
      1344-3542, 2186-2451
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Rapid identification and quantitation for oral bacteria based on short-end capillary electrophoresis2016

    • Author(s)
      Jin Chen, Yi Ni, Chenchen Liu, Yoshinori Yamaguchi, Qinmiao Chen, Shinichi Sekine, Xifang Zhu, Xiaoming Dou
    • Journal Title

      Talanta

      Volume: 160 Pages: 425-430

    • DOI

      10.1016/j.talanta.2016.07.049

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The Development of a Portable Buoyancy-Driven PCR System and Its Evaluation by Capillary Electrophoresis2016

    • Author(s)
      Zhenqing Li, Yang Zhao, Dawei Zhang, Songlin Zhuang, Yoshinori Yamaguchi
    • Journal Title

      Sensors & Actuators: B. Chemical

      Volume: 230 Pages: 779-784

    • DOI

      10.1016/j.snb.2016.02.143

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Analysis of the Inhibition of Nucleic Acid Dyes on Polymerase Chain Reaction by Capillary Electrophoresis2016

    • Author(s)
      Zhenqing Li, Chenchen Liu, Siyao Ma, Dawei Zhang, Yoshinori Yamaguchi
    • Journal Title

      Analytical Method

      Volume: 8 Issue: 11 Pages: 2330-2334

    • DOI

      10.1039/c5ay02705e

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Gene analysis of multiple oral bacteria by the polymerase chain reaction coupled with capillary polymer electrophoresis2016

    • Author(s)
      Liu C, Yamaguchi Y, Sekine S, Ni Y, Zhu X, Dou X.
    • Journal Title

      J Sep Sci

      Volume: 39(5) Issue: 5 Pages: 986-992

    • DOI

      10.1002/jssc.201501087

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Capillary electrophoresis of RNA in hydroxyethylcellulose polymer with various molecular weights.2016

    • Author(s)
      Zhenqing Li, Chenchen Liu, Dawei Zhang, Yoshinori Yamaguchi
    • Journal Title

      Journal of Chromatography B

      Volume: 1011 Pages: 114-120

    • DOI

      10.1016/j.jchromb.2015.12.057

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Gel-pump microfluidic system for chemical analysis2017

    • Author(s)
      Yoshinori Yamaguchi
    • Organizer
      17th Asia-Pacific International Symposium on Microscale Separations and Analysis(APCE2017) Shanghai
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ゲルポンプ内蔵マイクロチップ分析2017

    • Author(s)
      山口佳則
    • Organizer
      キャピラリー電気泳動シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Capillary Polymer Electrophoresis for nucleic acid2016

    • Author(s)
      Yoshinori Yamaguchi
    • Organizer
      2016 International Conference on Nanomaterials, Photoelectronic Devices and Information Technology
    • Place of Presentation
      Changzhou, China
    • Year and Date
      2016-11-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Capillary Electrophoresis Separation for 1-bp difference2016

    • Author(s)
      Yoshinori Yamaguchi
    • Organizer
      16th Asia-Pacific International Symposium on Microscale Separations and Analysis
    • Place of Presentation
      Johor Bahru, Johor, Malaysia
    • Year and Date
      2016-11-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] RNAキャピラリー電気泳動2016

    • Author(s)
      山口佳則
    • Organizer
      第67回日本電気泳動学会総会
    • Place of Presentation
      釧路観光国際交流センター
    • Year and Date
      2016-08-26
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 心筋細胞イメージング2016

    • Author(s)
      山口佳則
    • Organizer
      日本応用物理学会
    • Place of Presentation
      関西学院大学西宮キャンパス
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Capillary Electrophoresis for siRNA and Bacteria detection2015

    • Author(s)
      Yoshinori Yamaguchi
    • Organizer
      APCE 2015 (TAIWAN)
    • Place of Presentation
      TAIWAN
    • Year and Date
      2015-11-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2015-04-16   Modified: 2022-08-30  

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