| Project/Area Number |
15H03828
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
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| Research Collaborator |
KURODA Masao
OZAKI Shynsuke
SAKAMOTO Naoto
IMAICHI Yuki
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
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| Keywords | トロンビンバインディングアプタマー / カリウムイオン / ナトリウムイオン / 細胞外イメージング / 細胞イメージング / カリウム / ナトリウム / 4本鎖DNA / TBA / FRET / カリウムイオンセンシング / ナトリウムイオンセンシング / 蛍光プローブ |
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| Outline of Final Research Achievements |
Cell network signals were produced by ionic gradient between inner and outer living cell. Temporal-spatial imaging of ionic gradient of potassium (K+) and sodium (Na+) ions in living cell helps study of cell network signal. We successfully realized by K+ imaging by the oligonucleotide-peptide conjugate carrying fluorescence resonance energy transfer dye pair, named as potassium ion sensing oligonucleotide, PSO, for cell interior or cell network. We developed the method to locate PSO on a cell surface and to good performance for fluorescence intensity and preference of K+ over Na+. We synthesize series of PSO derivatives carrying different length of oligonucleotide and/or peptide. We constructed the method to localize PSO on a cell surface and found out PSO derivative carrying improved performance under cell surface. Furthermore, we change DNA sequence or length systematically to create sodium sensing oligonucleotide, SSO, and we synthesized it as a first case in the world.
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