| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
We have developed the signal amplification systems consisting of chemically-modified DNA conjugates. The luminescence and electrochemical signals were catalytically amplified with autonomous successive DNA strand exchanges such as HCR (hybridization chain reaction) and DNA circuits. The luminous signal amplification was performed with EDTA- and Phen (1,10-phenanthrorine)-modified DNA in the presence of lanthanide (Tb3+ or Eu3+) ion. The complexes formed on the certain DNA structures such as tandem duplex DNA, long tandem DNA wire, and cruciform DNA. Their luminescence lifetimes were extremely long as those of the typical luminous Ln complexes and their luminescence quantum yields were moderate, ca. 10 %. Electrochemical signals were amplified through entropy-driven DNA circuit consisting of Fc (ferrocene)- and βCyD (β-cyclodextrin)-modified DNA. We succeeded in enhancing the signals more by trapping Fc-DNA on the electrode modified with its complementary DNA.
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