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Development and applications of novel cell culture systems for quantitative and physiologically relevant evaluation of epithelial-mesenchymal transition

Research Project

Project/Area Number 15H03831
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionNational Institute for Materials Science

Principal Investigator

Nakanishi Jun  国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, グループリーダー (60360608)

Co-Investigator(Renkei-kenkyūsha) YAMAGUCHI Kazuo  神奈川大学, 理学部化学科, 教授 (20114902)
SUGAWARA Michiko  千葉大学, 工学部機械工学科, 准教授 (30323041)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywords細胞移動 / 上皮間葉転換 / がん / メカノバイオロジー / ケージド化合物
Outline of Final Research Achievements

Epithelial-mesenchymal transition (EMT) is a change in migration phenotype. It is thought as a promising target for cancer therapy. However, there was no convenient methods for the quantitative evaluation of EMT in physiologically relevant conditions. In the project, we revised our original photoactivatable substrates from the viewpoint of manipulating cellular mechanical environments and improving the responsivity of the photochemical reactions to make the platforms more suitable for the EMT assay. We have also succeeded in developing a mathematical method for the EMT quantification.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (28 results)

All 2018 2017 2016 2015

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 2 results,  Acknowledgement Compliant: 3 results) Presentation (16 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Book (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Photoactivatable substrates for systematic study of the impact of an extracellular matrix ligand on appearance of leader cells in collective cell migration2018

    • Author(s)
      S. A. Abdellatef and J. Nakanishi
    • Journal Title

      Biomaterials

      Volume: 169 Pages: 72-84

    • DOI

      10.1016/j.biomaterials.2018.03.045

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoactivatable Substrates: A Material-based Approach for Dissecting Cell Migration2017

    • Author(s)
      J. Nakanishi
    • Journal Title

      The Chemical Records

      Volume: 17 Issue: 6 Pages: 611-621

    • DOI

      10.1002/tcr.201600090

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of Myogenic Differentiation of Mammalian Cells Caused by Fluidity of a Liquid-Liquid Interface2017

    • Author(s)
      Kosuke Minami, Taizo Mori, Waka Nakanishi, Narumi Shigi, Jun Nakanishi, Jonathan P. Hill, Makoto Komiyama, Katsuhiko Ariga
    • Journal Title

      ACS APPLIED MATERIALS & INTERFACES

      Volume: 9 Issue: 36 Pages: 30553-30560

    • DOI

      10.1021/acsami.7b11445

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic Control of Cell Adhesion on a Stiffness-Tunable Substrate for Analyzing the Mechanobiology of Collective Cell Migration2016

    • Author(s)
      M. Kamimura, M. Sugawara, S. Yamamoto, K. Yamaguchi, and J. Nakanishi
    • Journal Title

      Biomaterials Science

      Volume: 4 Issue: 6 Pages: 933-937

    • DOI

      10.1039/c6bm00100a

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Reduced Adhesive Ligand Density in Engineered Extracellular Matrices Induces an Epithelial-Mesenchymal-Like Transition2016

    • Author(s)
      S. Marlar, S. A. Abdellatef, and J. Nakanishi
    • Journal Title

      Acta Biomaterialia

      Volume: 39 Pages: 106-113

    • DOI

      10.1016/j.actbio.2016.05.006

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Facile Preparation of Photoactivatable Surfaces with Tuned Substrate Adhesiveness2016

    • Author(s)
      Y. Shimizu, M. Kamimura, S. Yamamoto, S. A. Abdellatef, K. Yamaguchi, and J. Nakanishi
    • Journal Title

      Analytical Sciences

      Volume: 32 Issue: 11 Pages: 1183-1188

    • DOI

      10.2116/analsci.32.1183

    • NAID

      130005278006

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] What are the emerging concepts and challenges in NANO? Nanoarchitectonics, hand-operating nanotechnology and mechanobiology2016

    • Author(s)
      Katsuhiko Ariga, Kosuke Minami, Mitsuhiro Ebara and Jun Nakanishi
    • Journal Title

      Polymer Journal

      Volume: 48 Issue: 4 Pages: 371-389

    • DOI

      10.1038/pj.2016.8

    • NAID

      40020778218

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞メカノバイオロジーにおける力の計測方法2016

    • Author(s)
      中西淳
    • Journal Title

      ぶんせき

      Volume: 493 Pages: 25-26

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Facile preparation of a photoactivatable surface on a 96-well plate: a versatile and multiplex cell migration assay platform2015

    • Author(s)
      Masao Kamimura, Olivia Scheideler, Yoshihisa Shimizu, Shota Yamamoto, Kazuo Yamaguchi, Jun Nakanishi
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 17 Issue: 21 Pages: 14159-14167

    • DOI

      10.1039/c5cp01499a

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 細胞移動現象のアクティブウォッチング2018

    • Author(s)
      中西淳
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 表面の接着性に応じた細胞移動挙動を分析するための光応答基板の開発2017

    • Author(s)
      中西淳,山口和夫
    • Organizer
      第77回分析化学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 上皮間葉転換の阻害剤スクリーニングに向けた数理解析に基づく細胞集団性の定量化法2017

    • Author(s)
      深山達也,菅原道子,野々村真紀子,中西淳
    • Organizer
      分析化学会第66年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞集団移動の基質依存性の分析に用いる表面化学,力学特性が制御された光応答基板の開発2017

    • Author(s)
      岡田佳,山本翔太,山口和夫,佐々木直樹,中西淳
    • Organizer
      分析化学会第66年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 材料を用いる細胞メカノバイオロジーのアクティブウォッティング2017

    • Author(s)
      中西淳
    • Organizer
      分析化学会第66年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 細胞集団移動を探究するための光応答基板2017

    • Author(s)
      中西淳
    • Organizer
      細胞を創る会10.0
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 光応答材料を用いる細胞集団移動現象の構成的分析2017

    • Author(s)
      中西淳
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶応大学日吉キャンパス(神奈川県横浜市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Development of photoactivatable substrates and their applications to collective cell migration studies2016

    • Author(s)
      J. Nakanishi
    • Organizer
      ICBS2016
    • Place of Presentation
      伊藤ホール(東京都文京区)
    • Year and Date
      2016-11-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Photoactivatable compliant substrates for precise analysis of mechanobiological regulation in collective cell migration2016

    • Author(s)
      J. Nakanishi, M. Kamimura, K. Yamaguchi
    • Organizer
      WBC2016
    • Place of Presentation
      Montreal (Canada)
    • Year and Date
      2016-05-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞外マトリクスリガンドの密度低下によって誘導される上皮間葉転換2015

    • Author(s)
      中西淳,Marlar Saw
    • Organizer
      第37回日本バイオマテリアル学会
    • Place of Presentation
      京都
    • Year and Date
      2015-11-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 材料を用いる細胞集団移動現象の解析2015

    • Author(s)
      中西淳
    • Organizer
      第8回ChemBioハイブリッドレクチャー
    • Place of Presentation
      東京
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 光応答基板を用いる細胞集団移動現象におけるナノメカノバイオロジーの探究2015

    • Author(s)
      中西淳
    • Organizer
      第64回高分子討論会
    • Place of Presentation
      仙台
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞移動研究のためのマテリアルバイオロジー的研究ツール2015

    • Author(s)
      中西淳,山口和夫
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Photoactivatable substrates for dissecting biology and mechanics in collective cell migration2015

    • Author(s)
      Jun Nakanishi
    • Organizer
      International Symposium of Nanoarchitectonics for Mechanobiology
    • Place of Presentation
      つくば
    • Year and Date
      2015-07-29
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞移動アッセイのための市販96ウェルプレートの光機能化2015

    • Author(s)
      上村真生,菅原路子,山口和夫,中西淳
    • Organizer
      第64回高分子学会年次大会
    • Place of Presentation
      札幌
    • Year and Date
      2015-05-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光応答96ウェルプレートを用いる細胞移動アッセイ2015

    • Author(s)
      上村真生,菅原路子,山口和夫,中西淳
    • Organizer
      第75回分析化学討論会
    • Place of Presentation
      山梨
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Book] Biomaterials Nanoarchitectonics2016

    • Author(s)
      Jun Nakanishi
    • Total Pages
      347
    • Publisher
      Elsevier
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 上皮間葉転換誘導細胞阻害剤2016

    • Inventor(s)
      大村智,高橋洋子,中島琢自,松本厚子,中西淳
    • Industrial Property Rights Holder
      国立研究開発法人物質・材料研究機構/学校法人北里研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-186970
    • Filing Date
      2016-09-26
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] シャーレ型細胞培養容器2015

    • Inventor(s)
      山口和夫,伊藤倫子,山本翔太,中西淳,山本浩司
    • Industrial Property Rights Holder
      山口和夫,伊藤倫子,山本翔太,中西淳,山本浩司
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-08-26
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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