Development and applications of novel cell culture systems for quantitative and physiologically relevant evaluation of epithelial-mesenchymal transition
| Project/Area Number |
15H03831
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | National Institute for Materials Science |
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Principal Investigator |
Nakanishi Jun 国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, グループリーダー (60360608)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGUCHI Kazuo 神奈川大学, 理学部化学科, 教授 (20114902)
SUGAWARA Michiko 千葉大学, 工学部機械工学科, 准教授 (30323041)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Keywords | 細胞移動 / 上皮間葉転換 / がん / メカノバイオロジー / ケージド化合物 |
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| Outline of Final Research Achievements |
Epithelial-mesenchymal transition (EMT) is a change in migration phenotype. It is thought as a promising target for cancer therapy. However, there was no convenient methods for the quantitative evaluation of EMT in physiologically relevant conditions. In the project, we revised our original photoactivatable substrates from the viewpoint of manipulating cellular mechanical environments and improving the responsivity of the photochemical reactions to make the platforms more suitable for the EMT assay. We have also succeeded in developing a mathematical method for the EMT quantification.
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Report
(4 results)
Research Products
(28 results)
