Fluorescent probes for live-cell super-resolution imaging

Research Project

Project/Area Number	15H03833
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Bio-related chemistry
Research Institution	The University of Tokyo
Principal Investigator	Sato Moritoshi 東京大学, 大学院総合文化研究科, 教授 (00323501)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000) Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000) Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000) Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywords	蛍光 / イメージング / 細胞 / 超高解像度 / タンパク質
Outline of Final Research Achievements	We have developed a novel fluorescent probe for live-cell super-resolution imaging based on protein engineering of a blue-light photoreceptor. We have demonstrated its applicability to live-cell super-resolution imaging. The present study could allow us to visualize a broad range of biological processes at the super-resplition.