| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Outline of Final Research Achievements |
We have developed peptide nanocapsules (artificial viral capsids) self-assembled from beta-annulus peptide from tomato bushy stunt virus. In this research project, we developed nucleic acids-encapsulated artificial viral capsids bearing cell-penetrating peptides. We succeeded in synthesizing beta-annulus peptide bearing cell-penetrating His16 peptide at C-terminal, and encapsulating ssDNA into the artificial viral capsids modified with His16. The His16-modified artificial viral capsids were internalized well into HT1080 cells, whereas unmodified capsids hardly internalized. In addition, we have developed encapsulation of ssDNA via disulfide bonds into artificial viral capsids.
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