Clarification of fluid dynamical effect of near-wall flow field on enrtherial cell damage by measurement-integrated-simulation

• Project/Area Number: 15H03914
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fluid engineering
• Research Institution: Tohoku University
• Principal Investigator: Hayase Toshiyuki 東北大学, 流体科学研究所, 教授 (30135313)
• Co-Investigator(Kenkyū-buntansha): 西條 芳文 東北大学, 医工学研究科, 教授 (00292277) ; 下山 幸治 東北大学, 流体科学研究所, 准教授 (80447185)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
• Keywords: バイオ流体力学 / 計測融合シミュレーション

Outline of Final Research Achievements

It is known that damage on endothelial cells on the surface of a blood vessel causes diseases of blood vessels such as atherosclerosis or aneurisms. However, its mechanism is unknown. This study aimed to clarify the effect of flow field on the endothelial cells’ damage. A complex flow field near the surface of a carotid artery was reconstructed in a flow chamber and experiment of cell peeling by the flow load was conducted. Ultrasonic measurement integrated simulated was also performed to clarify the complex flow field in a carotid artery. Results obtained in this study are expected to be used in developing new diagnostic method of circulatory diseases based on understanding of fluid dynamical effect on cell damages.

Research Products

• [Journal Article] Photoplethysmography and ultrasonic-measurement-integrated simulation to clarify the relation between two-dimensional unsteady blood flow field and forward and backward waves in a carotid artery (2016)
  • Author(s): Shusaku Sone, Toshiyuki Hayase, Kenichi Funamoto, Atsushi Shirai
  • Journal Title: Medical & Biological Engineering & Computing Volume: - Issue: 5 Pages: 719-731
  • DOI: 10.1007/s11517-016-1543-4
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Study of Estimation Method for Unsteady Inflow Velocity in Two-Dimensional Ultrasonic-Measurement-Integrated Blood Flow Simulation (2016)
  • Author(s): Hiroko Kadowaki, Toshiyuki Hayase, Kenichi Funamoto, and Nobuyuki Taniguchi
  • Journal Title: IEEE Transactions on Biomedical Engineering Volume: 63 Issue: 2 Pages: 403-414
  • DOI: 10.1109/tbme.2015.2461559
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Blood flow analysis in carotid artery bifurcation by two-dimensional ultrasonic-measurement-integrated simulation (2015)
  • Author(s): Hiroko Kadowaki, Toshiyuki Hayase, Kenichi Funamoto, Shusaku Sone, Tadashi Shimazaki, Takao Jibiki, Koji Miyama
  • Journal Title: Journal of Biomechanical Science and Engineering Volume: 10 Issue: 1 Pages: 14-00266-14-00266
  • DOI: 10.1299/jbse.14-00266
  • NAID: 130004691140
  • ISSN: 1880-9863
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 血管壁近傍血流場が内皮細胞損傷に与える流体力学的影響の解明（流れ負荷実験における作動流体中の赤血球が内皮細胞の損傷に及ぼす影響） (2017)
  • Author(s): 井上浩介, 早瀬敏幸, 宮内優, 鈴木美利亞
  • Organizer: 日本機械学会第29回バイオエンジニアリング講演会
  • Place of Presentation: ウイングあいち（愛知県・名古屋市）
  • Year and Date: 2017-01-19
• [Presentation] 2次元超音波計測融合血流解析における高精度エイリアシング検出と補正 (2017)
  • Author(s): 原田大輔，早瀬敏幸，宮内優，井上浩介
  • Organizer: 日本機械学会第29回バイオエンジニアリング講演会
  • Place of Presentation: ウイングあいち（愛知県・名古屋市）
  • Year and Date: 2017-01-19
• [Presentation] 血管壁近傍血流場が内皮細胞損傷に与える流体力学的影響の解明(流れ負荷実験における作動流体中の赤血球が内皮細胞のはく離に及ぼす影響) (2017)
  • Author(s): 鈴木美利亜、早瀬敏幸、宮内優、井上浩介
  • Organizer: 日本機械学会第29回バイオエンジニアリング講演会
  • Place of Presentation: ウイングあいち（愛知県・名古屋市）
  • Year and Date: 2017-01-19
• [Presentation] Detection and Correction of Aliasing and Reverse Flow in Two-Dimensional Ultrasonic-Measurement-Integrated Blood Flow Analysis (2017)
  • Author(s): 15.Daisuke Harada, Toshiyuki Hayase, Suguru Miyauchi, Kosuke Inoue
  • Organizer: 14th International Conference on Flow Dynamics (ICFD2017)
  • Int'l Joint Research
• [Presentation] 2次元超音波計測融合血流解析における計測誤差の検出と補正 (2017)
  • Author(s): 原田大輔，早瀬敏幸，宮内優，井上浩介
  • Organizer: 東北大学・統計数理研究所合同ワークショップ
• [Presentation] 2次元超音波計測融合血流解析におけるエイリアシングと逆流の判別と補正 (2017)
  • Author(s): 原田大輔，早瀬敏幸，宮内優，井上浩介
  • Organizer: 日本超音波医学会第90回学術集会
• [Presentation] 2次元超音波計測融合血流解析により再現された血流場の可視化に関する研究 (2017)
  • Author(s): 原田大輔, 早瀬敏幸, 宮内優, 井上浩介
  • Organizer: 日本機械学会第30回バイオエンジニアリング講演会
• [Presentation] 血管壁近傍血流場が内皮細胞損傷に与える流体力学的影響の解明（斜流負荷実験における作動流体中の赤血球が内皮細胞のはく離に及ぼす影響） (2017)
  • Author(s): 井上浩介，早瀬敏幸，宮内優
  • Organizer: 日本機械学会第30回バイオエンジニアリング講演会
• [Presentation] Determination of Fluid Mechanical Effects Caused by Near Wall Blood Flow Field on Endothelial Cell Damage: Effect of Red Blood Cells in Working Fluid (2016)
  • Author(s): Miria Suzuki, Toshiyuki Hayase, Suguru Miyauchi, Kosuke Inoue
  • Organizer: 16th International Conference on Biomedical Engineering
  • Place of Presentation: Singapore（Singapore）
  • Year and Date: 2016-12-07
  • Int'l Joint Research
• [Presentation] Two-Dimensional Ultrasonic-Measurement-Integrated Simulation of Blood Flow in a Carotid Artery Considering Deformation of the Blood Vessel (2016)
  • Author(s): Daisuke Harada, Toshiyuki Hayase, Suguru Miyauchi, Kosuke Inoue
  • Organizer: 16th International Conference on Biomedical Engineering
  • Place of Presentation: Singapore（Singapore）
  • Year and Date: 2016-12-07
  • Int'l Joint Research
• [Presentation] 血管の変形を考慮した頸動脈血流の2次元超音波計測融合シミュレーション (2016)
  • Author(s): 原田大輔，早瀬敏幸，宮内優，井上浩介
  • Organizer: 日本機械学会第27回バイオフロンティア講演会
  • Place of Presentation: 北海道大学（北海道・札幌市）
  • Year and Date: 2016-10-22
• [Presentation] Two-Dimensional Ultrasonic-Measurement-Integrated Blood Flow Analysis Considering Deformation of Blood Vessel by Pulsation: Extraction of Unsteady Vessel Shape from B-mode Images (2016)
  • Author(s): Daisuke Harada, Toshiyuki Hayase, Suguru Miyauchi, Kosuke Inoue, Hiroko Kadowaki, Tadashi Shimazaki, Takao Jibiki, Koji Miyama
  • Organizer: 13th International Conference on Flow Dynamics (ICFD2016)
  • Place of Presentation: 仙台国際センター（宮城県・仙台市）
  • Year and Date: 2016-10-10
  • Int'l Joint Research
• [Presentation] Determination of Fluid Mechanical Effects Caused by Near Wall Blood Flow Field on Endothelial Cell Damage: Effect of Shear Stress on Cell Peeling of Cultured Endothelial Cells (2016)
  • Author(s): Miria Suzuki, Toshiyuki Hayase, Suguru Miyauchi, Kosuke Inoue
  • Organizer: 13th International Conference on Flow Dynamics (ICFD2016)
  • Place of Presentation: 仙台国際センター（宮城県・仙台市）
  • Year and Date: 2016-10-10
  • Int'l Joint Research
• [Presentation] 拍動に伴う血管の変形を考慮した2次元超音波計測融合血流解析システム (2016)
  • Author(s): 原田大輔，早瀬敏幸，宮内優，井上浩介，門脇弘子，船本健一，島崎正，地挽隆夫，見山広二
  • Organizer: 日本超音波医学会第89回学術集会
  • Place of Presentation: 国立京都国際会館、グランドプリンスホテル京都（京都府・京都市）
  • Year and Date: 2016-05-27
• [Presentation] 拍動に伴う血管形状の変形を考慮した2次元超音波計測融合血流解析（Bモード画像による非定常血管形状抽出） (2016)
  • Author(s): 原田大輔，早瀬敏幸，宮内優，井上浩介，門脇弘子，島崎正，地挽隆夫，見山広二
  • Organizer: 日本機械学会東北支部第51期総会・講演会
  • Place of Presentation: 東北大学（宮城県・仙台市）
  • Year and Date: 2016-03-11
• [Presentation] 2次元超音波計測融合血流解析における速度ベクトルの解析精度の検証 (2016)
  • Author(s): 門脇弘子，早瀬敏幸，宮内優，井上浩介，島崎正，地挽隆夫
  • Organizer: 日本機械学会第28回バイオエンジニアリング講演会
  • Place of Presentation: 東京工業大学（東京都・目黒区）
  • Year and Date: 2016-01-10
• [Presentation] Determination of optimum feedback gain of two-dimensional ultrasonic-measurement-integrated blood flow analysis system (2015)
  • Author(s): Hiroko Kadowaki, Toshiyuki Hayase, Kenichi Funamoto, Suguru Miyauchi, Kosuke Inoue, Tadashi Shimazaki, Takao Jibiki, Koji Miyama
  • Organizer: 12th International Conference on Flow Dynamics (ICFD2015)
  • Place of Presentation: 仙台国際センター（宮城県・仙台市）
  • Year and Date: 2015-10-29
  • Int'l Joint Research
• [Presentation] 2次元超音波計測融合血流解析における上流端流速推定手法の実血管形状による検証 (2015)
  • Author(s): 門脇弘子，早瀬敏幸，船本健一，宮内優，谷口信行
  • Organizer: 日本流体力学会年会2015
  • Place of Presentation: 東京工業大学（東京都・目黒区）
  • Year and Date: 2015-09-26
• [Presentation] Effect of speckle noise in ultrasonic measurement on two-dimensional ultrasonic-measurement-integrated blood flow analysis (2015)
  • Author(s): Hiroko Kadowaki, Toshiyuki Hayase, Kenichi Funamoto, Suguru Miyauchi, Kosuke Inoue, Tadashi Shimazaki, Takao Jibiki, Koji Miyama
  • Organizer: World Congress on Electrical Engineering and Computer Systems and Science
  • Place of Presentation: Barcelona （Spain）
  • Year and Date: 2015-07-13
  • Int'l Joint Research
• [Presentation] 2次元超音波計測融合血流解析システムにおける最適フィードバックゲインの決定 (2015)
  • Author(s): 門脇弘子，早瀬敏幸，船本健一，井上浩介，島崎正，地挽隆夫，見山広二
  • Organizer: 日本超音波医学会第88回学術集会
  • Place of Presentation: グランドプリンスホテル高輪（東京都・品川区）
  • Year and Date: 2015-05-22