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Elucidation of the proliferation and differentiation mechanism of the human induced pluripotent stem cells and its proposal to practical production from view point of transport phenomena

Research Project

Project/Area Number 15H04173
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Properties in chemical engineering process/Transfer operation/Unit operation
Research InstitutionOsaka University

Principal Investigator

Yasunori Okano  大阪大学, 基礎工学研究科, 教授 (90204007)

Co-Investigator(Kenkyū-buntansha) 紀ノ岡 正博  大阪大学, 工学研究科, 教授 (40234314)
金森 敏幸  国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (50356797)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Keywords移動速度論 / 数値解析 / iPS細胞 / 流動 / 物質移動 / 大量培養
Outline of Final Research Achievements

In suspension culture of iPS cells, the shear stress generated during the mixing is expected to promote differentiation of induced pluripotent stem (iPS) cells. This stress on the cell can be controlled by the impeller shape and rotational rate of the cell suspension. However, it is difficult to measure the effect of these operative parameters on the shear stress by experiments. Therefore, we have developed a numerical model to obtain the average and the maximum shear stress in two kinds of stirred tanks and an orbital shaking cylindrical container. Our results showed that the shear stress depended strongly on the type of mixing and lesser extent on the shape of the impeller. The average shear stress is larger in the shaking mode than that in the stirring mode. In contrast, the maximum shear stress is much smaller for the shaking than for stirring, suggesting stirring and shaking should be selectively used depending on the application.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (15 results)

All 2018 2017 2016 2015

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (13 results) (of which Int'l Joint Research: 5 results)

  • [Journal Article] Numerical investigation for the movement of cell colonies in bioreactors : stirring and orbital shaking tanks2018

    • Author(s)
      Takuya Yamamoto, Masaki Yano, Yasunori Okano and Masahiro Kino-oka
    • Journal Title

      J. Chem. Eng. Jpn.

      Volume: 未定

    • NAID

      130006743040

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Numerical study of fluid dynamics and particle behavior in an iPS cell culture tank2017

    • Author(s)
      Masaki Yano, Takuya Yamamoto, Yasunori Okano, Toshiyuki Kanamori and Mashiro Kino-oka
    • Journal Title

      AJChE

      Volume: 17 Pages: 29-36

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] iPS細胞懸濁培養における粒径分布の影響に関する数値解析2018

    • Author(s)
      田中 康奨, 矢野 雅貴, 関本 敦, 岡野 泰則, 紀ノ岡 正博
    • Organizer
      第20回化学工学会学生発表会(東広島大会)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 数値解析を用いたiPS細胞振盪培養における最適化条件の探索2018

    • Author(s)
      金丸 尚紀, 矢野 雅貴, 関本 敦, 岡野 泰則, 紀ノ岡 正博
    • Organizer
      化学工学会 第83年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 離散要素法を用いたiPS細胞懸濁培養の数値シミュレーション2017

    • Author(s)
      矢野雅貴、山本 卓也、岡野 泰則、都倉 知浩、金森 敏幸、紀ノ岡 正博
    • Organizer
      化学工学会第82年会
    • Place of Presentation
      芝浦工大(東京都江東区)
    • Year and Date
      2017-03-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 懸濁培養最適化のための数値流体解析・離散要素法連成シミュレーション2017

    • Author(s)
      矢野雅貴、山本 卓也、岡野 泰則、都倉 知浩、金森 敏幸、紀ノ岡 正博
    • Organizer
      化学工学会第82年会
    • Place of Presentation
      芝浦工大(東京都江東区)
    • Year and Date
      2017-03-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Numerical simulation of iPS cell behavior in a suspension culture using CFD-DEM2017

    • Author(s)
      Masaki Yano, Takuya Yamamoto, Yasunori Okano, and Mashiro Kino-oka
    • Organizer
      The 10th World Congress of Chemical Engineering (WCCE10)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] iPS細胞に関する懸濁培養最適化のための混合装置内粒子挙動解析モデル2017

    • Author(s)
      矢野 雅貴, 山本 卓也, 岡野 泰則, 紀ノ岡 正博
    • Organizer
      化学工学会第49回秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Numerical study of fluid dynamics and particle behaviour in an iPS cell culture tank2016

    • Author(s)
      M. Yano, T. Yamamoto, Y. Okano, T. Kanamori and M. Kino-Oka
    • Organizer
      23rd Regional Symposium on Chemical Engineering (RSCE2016)
    • Place of Presentation
      Vung Tau City, Vietnam
    • Year and Date
      2016-10-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Investigation of particle behavior in a stirred tank for optimization of suspension culture of iPS cell2016

    • Author(s)
      M. Yano, T. Yamamoto, Y. Okano, T. Kanamori, M. Kino-oka
    • Organizer
      The 27th International Symposium on Transport Phenoma (ISTP-27)
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2016-09-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] iPS細胞懸濁培養最適化のための粒子挙動の数値解析2016

    • Author(s)
      矢野 雅貴、山本 卓也、高木 洋平、岡野 泰則、杉浦 慎治、金森 敏幸、紀ノ岡 正博
    • Organizer
      化学工学会 第48回秋季大会
    • Place of Presentation
      徳島大学(徳島県徳島市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 培養環境中の三次元物質移動を考慮したiPS細胞培養数値シミュレーション2016

    • Author(s)
      鈴木寛子、山本卓也、高木洋平、岡野泰則、杉浦慎治、須丸公雄、金森敏幸、紀ノ岡正博
    • Organizer
      化学工学会第81年会
    • Place of Presentation
      関西大学(大阪府吹田市)
    • Year and Date
      2016-03-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Numerical modeling of iPS cell culture based on mass transfer in a culture environment2015

    • Author(s)
      H. Suzuki, T. Yamamoto, Y. Takagi, Y. Okano, S. Sugiura, K. Sumaru, T. Kanamori and M. Kino-oka
    • Organizer
      APCChE 2015 Congress incorporating Chemeca 2015
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2015-09-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Numerical Modelling on iPS Cell Culture Based on Transport Phenomena in a Culture Environment2015

    • Author(s)
      Y. Okano, H. Suzuki, T. Yamamoto, Y. Takagi, S. Sugiura, K. Sumaru, T. Kanamori, and M. Kino-oka
    • Organizer
      The 22nd Regional Symposium on Chemical Engineering (RSCE 2015)
    • Place of Presentation
      Vung Tau City, Vietnam
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] iPS細胞のかん流培養に関する数値シミュレーション2015

    • Author(s)
      鈴木寛子、山本卓也、高木洋平、岡野泰則、杉浦慎治、須丸公雄、金森敏幸、紀ノ岡正博
    • Organizer
      化学工学会第47回秋季大会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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