| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
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| Outline of Final Research Achievements |
All of the > 30,000 trierpenoinds are synthesized via squalene. To start accessing those structurally and functionally diverse compounds, we have developed a novel screening system to visualize squalene consumption. By using this as a screening principle, we have identified activity mutants of squalene cyclizing enzymes, enabling efficient microbial synthesis of cyclic triterpenoids. By combining these mutant enzymes with pathways for squalene-like molecules, we succeeded in establishing pathways toward numbers of novel compounds that have not been previously reported.
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