Directed evolution of squalene-converting biosynthetic pathways
| Project/Area Number |
15H04189
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
Umeno Daisuke 千葉大学, 大学院工学研究院, 准教授 (00400812)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
河合 繁子 千葉大学, 大学院工学研究院, 助教 (40638920)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
|
|---|
| Keywords | 合成生物学 / 進化分子工学 / スクリーニング / トリテルペン / オキシドスクアレン / 環化酵素 / 応用微生物 / バイオテクノロジー / 生物・生体工学 / 酵素反応 / 生体分子 / 微生物 / 生体機能利用 / 進化 / スクアレン / ホペン / スクアラン / ライブラリ / セレクション |
|---|
| Outline of Final Research Achievements |
All of the > 30,000 trierpenoinds are synthesized via squalene. To start accessing those structurally and functionally diverse compounds, we have developed a novel screening system to visualize squalene consumption. By using this as a screening principle, we have identified activity mutants of squalene cyclizing enzymes, enabling efficient microbial synthesis of cyclic triterpenoids. By combining these mutant enzymes with pathways for squalene-like molecules, we succeeded in establishing pathways toward numbers of novel compounds that have not been previously reported.
|
Report
(2 results)
Research Products
(8 results)
-
-
-
-
[Journal Article] Production of squalene by squalene synthases and their truncated mutants in Escherichia coli.2015
Author(s)
Katabami, A, Li, L., Iwasaki, M., Furubayashi, M., Saito, K., Umeno, D
-
Journal Title
J. Bioscience Bioengin
Volume: 119
Issue: 2
Pages: 165-171
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
-
-
-
