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Development of bioactive cell interface using peptides and nano-materials

Research Project

Project/Area Number 15H04192
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionTokyo Institute of Technology

Principal Investigator

Okochi Mina  東京工業大学, 物質理工学院, 教授 (70313301)

Co-Investigator(Kenkyū-buntansha) 田中 祐圭  東京工業大学, 物質理工学院, 助教 (60533958)
早水 裕平  東京工業大学, 物質理工学院, 准教授 (80443216)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywordsペプチド / 細胞界面 / ナノ材料 / バイオセンサ / ナノバイオ / ナノシート / 先端機能デバイス
Outline of Final Research Achievements

Two-dimensional materials such as graphene, an individual layer of graphite with only an atom thickness, have attracted great attention due to its superior electrical properties. Along with these properties, increasing exploration on the potential applications of graphene and its derivatives are being utilized in many areas including electronic and energy storage devices, advanced materials, biomedicine, and biotechnology. In this study, self-assembling peptides have been focused as the non-covalent functionalization biointerface material, and peptides self-assembling to the surface of thin layer of pyropytic graphite was designed and bi-functionalized by conjugating to the peptide sequences showing affinity to cell membrane.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (32 results)

All 2018 2017 2016 2015 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 4 results,  Open Access: 3 results) Presentation (22 results) (of which Int'l Joint Research: 6 results,  Invited: 3 results) Remarks (1 results) Funded Workshop (1 results)

  • [Journal Article] Screening of bacteria-binding peptides and one-pot ZnO surface modification for bacterial cell entrapment.2018

    • Author(s)
      Masayoshi Tanaka, Ilva Hanun Harlisa, Yuta Takahashi, Natasha Agustin Ikhsana, Mina Okochi
    • Journal Title

      RSC Adv.

      Volume: 8 Issue: 16 Pages: 8795-8799

    • DOI

      10.1039/c7ra12302g

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Screening of peptide probe binding to particulate matter with a high metal content.2018

    • Author(s)
      Masayoshi Tanaka, Aw Wei Liang Alvin, Mina Okochi
    • Journal Title

      RSC Adv.

      Volume: 8 Issue: 11 Pages: 5953-5959

    • DOI

      10.1039/c7ra13290e

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection of Her2-overexpressing cancer cells using the on-chip RT-PCR employing a magnetic droplet-manipulation system.2017

    • Author(s)
      96.Mina Okochi, Shinji Koike, Masayoshi Tanaka, Hiroyuki Honda
    • Journal Title

      Biosens. Bioelectron.

      Volume: 93 Pages: 32-39

    • DOI

      10.1016/j.bios.2016.11.013

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Array-based functional peptide screening and characterization of gold nanoparticle synthesis.2017

    • Author(s)
      Tanaka M, Hikiba S, Yamashita K, Muto M, Okochi M.
    • Journal Title

      Acta Biomater.

      Volume: 49 Pages: 495-506

    • DOI

      10.1016/j.actbio.2016.11.037

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Characterization of metal binding properties of PM2.5 binding peptides screened from phage display.2017

    • Author(s)
      Aw Wei Liang Alvin, Masayoshi Tanaka, Mina Okochi
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 123 Issue: 5 Pages: 621-624

    • DOI

      10.1016/j.jbiosc.2016.12.014

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Screening of peptides associated with adhesion and aggregation of Lactobacillus rhamnosus GG in vitro.2017

    • Author(s)
      Mina Okochi, Tomoya Sugita, Yuji Asai, Masayoshi Tanaka, Hiroyuki Honda
    • Journal Title

      Biochem. Eng. J.

      Volume: 128 Pages: 178-185

    • DOI

      10.1016/j.bej.2017.10.004

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid Colorimetric Antibody Detection Using a Dual-function Peptide Probe for Silver Nanoparticle Aggregation and Antibody Recognition2016

    • Author(s)
      Mina Okochi, Tomohiro Kamiya, Takeshi Omasa, Masayoshi Tanaka, Hiroyuki Honda
    • Journal Title

      Analytical Sciences

      Volume: 32 Issue: 1 Pages: 93-97

    • DOI

      10.2116/analsci.32.93

    • NAID

      130005118571

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A molecular peptide beacon for IgG detection.2015

    • Author(s)
      Mina Okochi, Tomoya Sugita, Masayoshi Tanaka, Hiroyuki Honda
    • Journal Title

      RSC Adv.

      Volume: 5 Issue: 112 Pages: 91988-91992

    • DOI

      10.1039/c5ra15174k

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] グラフェン上でβ-sheet構造を形成するペプチドを利用した電極上への細胞固定2018

    • Author(s)
      立松宗一郎、 田中祐圭、大河内美奈
    • Organizer
      化学工学会第83年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Screening and characterization of Particulate-matter-binding peptides2018

    • Author(s)
      M. Okochi
    • Organizer
      Biotechnological Routes to Multifunctional Materials
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細胞の電気化学測定に向けたペプチド界面設計2017

    • Author(s)
      立松宗一郎、 田中祐圭、大西知子、早水裕平、大河内美奈
    • Organizer
      化学工学会第82年会
    • Place of Presentation
      芝浦工業大学
    • Year and Date
      2017-03-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] 膜結合ペプチドを用いたグラフェン電極への生体膜の固定2017

    • Author(s)
      立松 宗一郎、田中 祐圭、大西 知子、早水 裕平、大河内 美奈
    • Organizer
      化学とマイクロ・ナノシステム学会第35研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 金ナノ粒子合成制御に向けた金イオン還元性を示すペプチド触媒ライブラリーの作製2017

    • Author(s)
      田中 祐圭、大河内美奈
    • Organizer
      日本生物工学会第69回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 合成される金ナノ粒子物性を選択できるペプチド触媒のスクリーニング2017

    • Author(s)
      田中 祐圭、大河内美奈
    • Organizer
      日本化学会第11回バイオ関連化学シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] ペプチドマイクロアレイ解析技術によるアレルギー症状の経過予測2017

    • Author(s)
      大河内美奈
    • Organizer
      日本生物工学会第69回大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Peptide array-based rational design of TNT recognition peptide derived from anti-TNT monoclonal antibody2017

    • Author(s)
      M. Okochi, K. Yanai, M. Muto, M. Tanaka
    • Organizer
      10th World Congress of Chemical Engineering
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gold nanoparticle synthesis using multi-functional peptide screened by peptide array2017

    • Author(s)
      M. Tanaka, M. Okochi
    • Organizer
      10th World Congress of Chemical Engineering
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Peptide screening for one-pot gold nanopaticle syntheses2017

    • Author(s)
      M. Tanaka, M. Okochi
    • Organizer
      YABEC2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 金ナノ粒子合成に寄与する二機能性ペプチドのキャラクタリゼーション2016

    • Author(s)
      田中 祐圭、大河内 美奈
    • Organizer
      第68回日本生物工学会
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 複合機能性ペプチドを利用した金ナノ粒子合成法の開発2016

    • Author(s)
      田中祐圭、武藤正記、大河内美奈
    • Organizer
      化学工学会第48回秋季大会
    • Place of Presentation
      徳島大学
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] ペプチドプローブを用いたバイオセンシング2016

    • Author(s)
      大河内美奈
    • Organizer
      2016ペプチド夏の勉強会
    • Place of Presentation
      八王子セミナーハウス
    • Year and Date
      2016-07-31
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Detection of cancer cells using on-chip gene expression analysis utilizing a magnetic beads-droplet-manipulation system2016

    • Author(s)
      Mina Okochi, Shinji Koike, Masayoshi Tanaka, Hiroyuki Honda
    • Organizer
      Biosensors 2016
    • Place of Presentation
      Gothenburg, Sweden
    • Year and Date
      2016-05-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Screening of bacterial lipopolysaccharide binding peptides from toll like receptor 4-derived peptide library2016

    • Author(s)
      Ilva Hanun Harlisa, Masaki Muto, Masayoshi Tanaka, Mina Okochi
    • Organizer
      ImPACT International Symposium on InSECT 2016
    • Place of Presentation
      名古屋大学
    • Year and Date
      2016-04-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] Peptide array based screening of gold binding peptides that catalyze gold nanoparticle production2016

    • Author(s)
      Masayoshi Tanaka, Mina Okochi
    • Organizer
      ImPACT International Symposium on InSECT 2016
    • Place of Presentation
      名古屋大学
    • Year and Date
      2016-04-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 金ナノ粒子合成に寄与するペプチドのキャラクタリゼーション2016

    • Author(s)
      田中 祐圭、山下 健仁、大河内美奈
    • Organizer
      化学とマイクロ・ナノシステム学会 第33回研究会
    • Place of Presentation
      東京大学生産技術研究所
    • Year and Date
      2016-04-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 乳酸菌およびグラファイトに親和性を示す二機能性ペプチドの設計2016

    • Author(s)
      大河内美奈、大西知子、増島弘顕、田中祐圭、早水裕平
    • Organizer
      電気化学会第83回大会
    • Place of Presentation
      大阪大学
    • Year and Date
      2016-03-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] Toll like receptor 4 からの大腸菌結合ペプチドプローブの探索2016

    • Author(s)
      Ilva Hanun Harlisa、高橋雄太、武藤正記、田中祐圭、大河内美奈
    • Organizer
      化学工学会第81回大会
    • Place of Presentation
      関西大学
    • Year and Date
      2016-03-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] In vitro 3D co-culture patterning model for evaluation of tumor cell malignancy2015

    • Author(s)
      Mina Okochi, Shuhei Yamamoto, Hiroyuki Honda
    • Organizer
      PACIFICHEM2015
    • Place of Presentation
      大阪大学
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 乳酸菌と親和性を持つグラファイト結合ペプチドの評価2015

    • Author(s)
      大西知子、増島弘顕、田中祐圭、大河内美奈、早水裕平
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      城山観光ホテル
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Toll-like receptor 4 (TLR4) 由来ペプチドライブラリーによる細菌結合ペプチドの探索2015

    • Author(s)
      高橋 雄太、 Ilva Hanun Harlisa、 田中 祐圭、 大河内 美奈
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      城山観光ホテル
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Remarks] 東京工業大学物質理工学院大河内研究室

    • URL

      http://www.chemeng.titech.ac.jp/~lab-okochi/index.html

    • Related Report
      2017 Annual Research Report 2015 Annual Research Report
  • [Funded Workshop] Biosensors20162016

    • Place of Presentation
      Gothenburg, Sweden
    • Year and Date
      2016-05-25
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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