• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of cellular and nuclear signals that regulate motor learning

Research Project

Project/Area Number 15H04254
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurophysiology / General neuroscience
Research InstitutionGunma University

Principal Investigator

Hirai Hirokazu  群馬大学, 大学院医学系研究科, 教授 (70291086)

Co-Investigator(Renkei-kenkyūsha) HOSOI Nobutake  群馬大学, 大学院医学系研究科, 講師 (90543570)
KONNO Ayumu  群馬大学, 大学院医学系研究科, 講師 (40509048)
Research Collaborator MATSUZAKI Yasunori  群馬大学, 大学院医学系研究科, 助教 (50738200)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Keywords小脳 / プルキンエ細胞 / mGluR1 / 運動学習 / バクロフェン / 脊髄小脳失調症 / 運動失調 / AAVベクター / SCA1 / SCA3 / 運動記憶 / 脳神経疾患 / 遺伝子 / シグナル伝達 / 神経科学 / 生理学 / 脊髄小脳変性症 / ポリグルタミン病 / ロータロッド / パッチクランプ
Outline of Final Research Achievements

Decrease of several genes including transient receptor potential cation channel type 3 (TRPC3), inositol trisphosphate (IP3) receptor and Homer protein homolog 3 (HOMER3) were found in the cerebellum of spinocerebellar ataxia type 1 (SCA1) knock-in mouse. We identified retinoid-related orphan receptor α (RORα), which is a transcription factor that could regulate expression of those genes. Moreover, we found that a gene “X” is increased more than 3 times in SCA1 knock-in mice 3 h after treatment with baclofen and rotarod training. Since haploinsufficiency of the gene “X” is known to cause mental retardation, upregulation of the gene “X” may play a key role in baclofen-induced improvement of motor learning in SCA1 mice.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (42 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (15 results) Journal Article (14 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 14 results,  Acknowledgement Compliant: 2 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results) Remarks (1 results)

  • [Int'l Joint Research] Krasnoyarsk State Medical University(ロシア連邦)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Universitas Padjadjaran(インドネシア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Bergen(ノルウェー)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Max-Planck Florida Institute/Oregon Health & Science University(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] WaBios(シンガポール)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Oregon Health & Science University/Max-Planck Florida Institute(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Toronto(Canada)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] WaBios(Singapore)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Bergen(Norway)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Coimbra(Portugal)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] コインブラ大学(ポルトガル)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] クラスノヤルスク医科大学(ロシア連邦)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] パジャジャラン大学(インドネシア)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] トロント大学(カナダ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Intravenous administration of the adeno-associated virus-PHP.B capsid fails to upregulate transduction efficiency in the marmoset brain2018

    • Author(s)
      Matsuzaki Yasunori、Konno Ayumu、Mochizuki Ryuta、Shinohara Yoichiro、Nitta Keisuke、Okada Yukihiro、Hirai Hirokazu
    • Journal Title

      Neuroscience Letters

      Volume: 665 Pages: 182-188

    • DOI

      10.1016/j.neulet.2017.11.049

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Type 1 metabotropic glutamate receptor and its signaling molecules as therapeutic targets for the treatment of cerebellar disorders2018

    • Author(s)
      Hirai H, Kano M.
    • Journal Title

      Curr Opin Pharmacol

      Volume: 38 Pages: 51

    • DOI

      10.1016/j.coph.2018.02.002

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CD38 positively regulates postnatal development of astrocytes cell-autonomously and oligodendrocytes non-cell-autonomously.2017

    • Author(s)
      Hattori T, Kaji M, Ishii H, Jureepon R, Takarada-Iemata M, Ta HM, Le TM, Konno A, Hirai H, Shiraishi Y, Ozaki N, Yamamoto Y, Okamoto H, Yokoyama S, Higashida H, Kitao Y, Hori O.
    • Journal Title

      Glia

      Volume: 印刷中 Issue: 6 Pages: 974-989

    • DOI

      10.1002/glia.23139

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging2017

    • Author(s)
      Harada Kazuki、Ito Motoki、Wang Xiaowen、Tanaka Mika、Wongso Devina、Konno Ayumu、Hirai Hirokazu、Hirase Hajime、Tsuboi Takashi、Kitaguchi Tetsuya
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 7351-7351

    • DOI

      10.1038/s41598-017-07820-6

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Minimal Purkinje Cell-Specific PCP2/L7 Promoter Virally Available for Rodents and Non-human Primates2017

    • Author(s)
      Nitta Keisuke、Matsuzaki Yasunori、Konno Ayumu、Hirai Hirokazu
    • Journal Title

      Molecular Therapy - Methods & Clinical Development

      Volume: 6 Pages: 159-170

    • DOI

      10.1016/j.omtm.2017.07.006

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulatory connection between the expression level of classical protein kinase C and pruning of climbing fibers from cerebellar Purkinje cells2017

    • Author(s)
      Takahashi Nobutaka、Shuvaev Anton N.、Konno Ayumu、Matsuzaki Yasunori、Watanave Masashi、Hirai Hirokazu
    • Journal Title

      Journal of Neurochemistry

      Volume: 143 Issue: 6 Pages: 660-670

    • DOI

      10.1111/jnc.14239

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Protein Kinase C in the Cerebellum: Its Significance and Remaining Conundrums.2017

    • Author(s)
      Hirai H.
    • Journal Title

      Cerebellum

      Volume: 17 Issue: 1 Pages: 23-27

    • DOI

      10.1007/s12311-017-0898-x

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Progressive impairment of cerebellar mGluR signalling and its therapeutic potential for cerebellar ataxia in spinocerebellar ataxia type 1 model mice.2017

    • Author(s)
      Shuvaev AN, Hosoi N, Sato Y, Yanagihara D, Hirai H.
    • Journal Title

      The Journal of Physiology

      Volume: 595(1) Issue: 1 Pages: 141-164

    • DOI

      10.1113/jp272950

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Caffeine alleviates progressive motor deficits in a transgenic mouse model of spinocerebellar ataxia.2017

    • Author(s)
      Goncalves N, Simoes AT, Prediger RD, Hirai H, Cunha RA, Pereira de Almeida L
    • Journal Title

      Ann Neurol

      Volume: 81 Issue: 3 Pages: 407-418

    • DOI

      10.1002/ana.24867

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Morphological and Functional Attenuation of Degeneration of Peripheral Neurons by Mesenchymal Stem Cell-Conditioned Medium in Spinocerebellar Ataxia Type 1-Knock-in Mice.2016

    • Author(s)
      Suto N, Mieda T, Iizuka A, Nakamura K, Hirai H.
    • Journal Title

      CNS Neurosci Ther

      Volume: 22 Issue: 8 Pages: 670-676

    • DOI

      10.1111/cns.12560

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] FMRP Expression Levels in Mouse CNS Neurons Determine Behavioral Phenotype.2016

    • Author(s)
      Arsenault J, Gholizadeh S, Niibori Y, Pacey LK, Halder SK, Koxhioni E, Konno A, Hirai H, Hampson DR
    • Journal Title

      Human Gene Therapy

      Volume: 27 Issue: 12 Pages: 982-996

    • DOI

      10.1089/hum.2016.090

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Plasticity of the developmentally arrested staggerer cerebellum in response to exogenous RORα.2016

    • Author(s)
      Iizuka A, Matsuzaki Y, Konno A, Hirai H.
    • Journal Title

      Brain Structure and Function

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuropeptide Y mitigates neuropathology and motor deficits in mouse models of Machado-Joseph disease.2015

    • Author(s)
      Duarte-Neves J, Goncalves N, Cunha-Santos J, Simoes AT, den Dunnen WF, Hirai H, Kugler S, Cavadas C, Pereira de Almeida L.
    • Journal Title

      Hum Mol Genet

      Volume: 24 Issue: 19 Pages: 5451-5463

    • DOI

      10.1093/hmg/ddv271

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Re-establishing ataxin-2 downregulates translation of mutant ataxin-3 and alleviates Machado-Joseph disease.2015

    • Author(s)
      Nobrega C, Carmo-Silva S, Albuquerque D, Vasconcelos-Ferreira A, Vijayakumar UG, Mendonca L, Hirai H, Pereira de Almeida L.
    • Journal Title

      Brain

      Volume: 138 Issue: 12 Pages: 3537-3554

    • DOI

      10.1093/brain/awv298

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Pros and cons of an AAV double infection method combined with the Tet system: An experimental study2017

    • Author(s)
      Ayumu Konno, Hirokazu Hirai
    • Organizer
      Neuroscience 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of cerebellar function by protein kinase C2017

    • Author(s)
      Hirokazu Hirai
    • Organizer
      International Symposium of the Society for Research on the Cerebellum and Ataxias
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] In vivo evaluation of efficacy of the shortened WPRE sequence in cerebellum2017

    • Author(s)
      Ayumu Konno, Kazuma Hachisu, Rika terashima, Hirokazu Hirai
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Low concentration of a GABAb receptor agonist improves motor performance in spinocerebellar ataxia type 1 (SCA1) model mice2017

    • Author(s)
      Nobutake Hosoi, Shuvaev N Anton, Hirokazu Hirai
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] AAV9 vector-mediated production of mouse model of spinocerebellar ataxia type 3 and electrophysiological analysis of the mouse2017

    • Author(s)
      Chiaki Hoshino, Masashi Watanabe, Ayumu Konno, Hirokazu Hirai
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Regulation of the firing property in mature Purkinje cells by PKC γ contributes to the motor coordination2017

    • Author(s)
      Masashi Watanabe, Nobutaka Takahashi, Ayumu Konno, Hirokazu Hirai
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 小脳プルキンエ細胞における古典的Cキナーゼαとγの発現量と生理機能2017

    • Author(s)
      平井宏和
    • Organizer
      第39回日本生物学的精神医学会・第47回日本神経精神薬理学会 合同年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Progressive abnormalities in short-and long term synaptic plasticity and dendritic Ca2+ signals in spinocerebellar ataxia type 1 (SCA1) model mice.2016

    • Author(s)
      細井延武、シュワエフ アントン、平井宏和
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌コンベンションセンター (北海道札幌市)
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Progressive impairment of metabotropic glutamate receptor(mGluR) mediated signaling and carebellar synaptic plasticity in spinocerebellar ataxia type 1(SCA1) model mice2016

    • Author(s)
      Nobutake Hosoi, Anton N Shuvaev, Hirokazu Hirai
    • Organizer
      Neuroscience 2016
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Generation of a marmoset model of spinocerebellar ataxia type 3 via AAV9 vector-mediated gene transfer2016

    • Author(s)
      Ayumu Konno, Yasunori Matsuzaki, Hirokazu Hirai
    • Organizer
      Neuroscience 2016
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Progressive impairment of cerebellar mGluR signaling in SCA1 model mice.2015

    • Author(s)
      Nobutake Hosoi, Hirokazu Hirai.
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸国際会議場 (兵庫県神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Gene and stem cell therapies against polyglutamine diseases.2015

    • Author(s)
      平井宏和
    • Organizer
      第56回日本神経学会学術大会
    • Place of Presentation
      朱鷺メッセ (新潟県新潟市)
    • Year and Date
      2015-05-20
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Remarks] 群馬大学大学院医学系研究科脳神経再生医学分野

    • URL

      http://synapse.dept.med.gunma-u.ac.jp/

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report 2015 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2022-08-31  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi