| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
Drosophila larval peristalsis is generated by sequential contractions of body-wall muscles from the posterior to anterior segments. In this study, we used connectomics and optogenetics to identify excitatory premotor interneurons in this system, termed CLIs. These neurons were segmentally arrayed and activated sequentially from the posterior to anterior segments during peristalsis. The CLIs express ChAT, a marker for cholinergic neurons and were active just prior to motoneuronal firing in each segment. Moreover, local activation of CLI1s induced contraction of muscles in the corresponding body segments. Our results suggest that the CLIs directly activate motoneurons sequentially along the segments during larval locomotion.
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