Column maturation and neural activity in mouse somatosensory cortex L4

• Project/Area Number: 15H04263
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: National Institute of Genetics
• Principal Investigator: IWASATO Takuji 国立遺伝学研究所, 個体遺伝研究系, 教授 (00311332)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2015: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 神経科学 / 神経回路 / マウス / 自発活動 / 回路形成 / イメージング / 発達 / 脳 / 体性感覚野 / in vivo

Outline of Final Research Achievements

In order to understand the role of neural activity in columnar maturation in the neocortex, whisker-barrel system in the mouse was used as a model. Our in vivo imaging analyses using the GCaMP6s have revealed spontaneous activity with a unique spatio-temporal pattern in the neonatal mouse barrel cortex.

Academic Significance and Societal Importance of the Research Achievements

ヒトを含む哺乳類の脳では膨大な数のニューロンが回路を形成し、多様な情報の処理や行動の制御を行っている。そうした複雑な神経回路は、ゲノム情報によって大まかに作られた後、主に生後に実際に使われる中で調整され、成熟する。今回、そうした子供期の脳における回路試運転の実態の一端が、マウス体性感覚野をモデルとした研究で明らかになった。この成果は、ヒトの正常な子供の脳の発達の理解、および、その破綻による発達障害や精神疾患の理解にもつながることが期待される。

Research Products

• [Journal Article] Differential dynamics of cortical neuron dendritic trees revealed by long-term in vivo imaging in neonates (2018)
  • Author(s): Nakazawa Shingo、Mizuno Hidenobu、Iwasato Takuji
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 3106-3106
  • DOI: 10.1038/s41467-018-05563-0
  • Peer Reviewed / Open Access
• [Journal Article] In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice (2018)
  • Author(s): Mizuno H*, Nakazawa S, Iwasato T (* correspondence)
  • Journal Title: Journal of Visualized Experiments Volume: 140 Issue: 140
  • DOI: 10.3791/58340
  • Peer Reviewed / Open Access
• [Journal Article] Patchwork-type spontaneous activity in neonatal barrel cortex layer 4 transmitted via thalamocortical projections. (2018)
  • Author(s): Mizuno, H., Ikezoe, K., Nakazawa, S., Sato, T., Kitamura, K., Iwasato, T.
  • Journal Title: Cell Rep. Volume: 22 Issue: 1 Pages: 123-135
  • DOI: 10.1016/j.celrep.2017.12.012
  • Peer Reviewed / Open Access
• [Journal Article] Development of tactile sensory circuits in the CNS (2018)
  • Author(s): Iwasato Takuji、Erzurumlu Reha S.
  • Journal Title: Current Opinion in Neurobiology Volume: 53 Pages: 66-75
  • DOI: 10.1016/j.conb.2018.06.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Protocadherin-αC2 is required for diffuse projections of serotonergic axons (2017)
  • Author(s): Katori Shota、Noguchi-Katori Yukiko、Okayama Atsushi、Kawamura Yoshimi、Luo Wenshu、Sakimura Kenji、Hirabayashi Takahiro、Iwasato Takuji、Yagi Takeshi
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 15908-15908
  • DOI: 10.1038/s41598-017-16120-y
  • Peer Reviewed / Open Access
• [Journal Article] Spinal RacGAP α-Chimaerin Is Required to Establish the Midline Barrier for Proper Corticospinal Axon Guidance (2017)
  • Author(s): Katori Shota、Noguchi-Katori Yukiko、Itohara Shigeyoshi、Iwasato Takuji
  • Journal Title: The Journal of Neuroscience Volume: 37 Issue: 32 Pages: 7682-7699
  • DOI: 10.1523/jneurosci.3123-16.2017
  • Peer Reviewed
• [Journal Article] Prenatal thalamic waves regulate cortical area size prior to sensory processing. (2017)
  • Author(s): Moreno-Juan, V., Filipchuk, A., Antoｎ-Bolarios, N., Mezzera, C., Gezelius, H., Andes, B., Rodriguez-Malmierca, L., Susin, R., Schaad, O., Iwasato, T., Schuele, R., Rutlin, M., Nelson, S., Ducret, S., Valdeolmillos, M., Rijli, F., Lopez-Bendito, G.
  • Journal Title: Nature Commun. Volume: 8 Issue: 1 Pages: 14172-14172
  • DOI: 10.1038/ncomms14172
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. (2016)
  • Author(s): Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S., Iwasato, T.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 35747-35747
  • DOI: 10.1038/srep35747
  • Peer Reviewed / Open Access
• [Journal Article] In vitro Assay for Dendritic Spine Retraction of Hippocampal Neurons with Sparse Labeling. (2016)
  • Author(s): Iwata, R., Iwasato, T
  • Journal Title: Bio-Protocol Volume: 6
  • Peer Reviewed / Open Access
• [Journal Article] CAPS1 stabilizes the state of readily releasable synaptic vesicles to fusion competence at CA3-CA1 synapses in adult hippocampus (2016)
  • Author(s): 6.Shinoda Y, Ishii C, Fukazawa Y, Sadakata T, Ishii Y, Sano Y, Iwasato T, Itohara S, Furuichi T
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 31540-31540
  • DOI: 10.1038/srep31540
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Developmental RacGAPa 2-chimaerin signaling is a determinant of the morphological features of dendritic spines in adulthood. (2015)
  • Author(s): .Iwata, R., Matsukawa, H., Yasuda, K., Mizuno, H., Itohara, S., Iwasato, T.
  • Journal Title: J. Nerurosci. Volume: 35 Issue: 40 Pages: 13728-13744
  • DOI: 10.1523/jneurosci.0419-15.2015
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 学習能力の発達を調節するタンパク質-成長期におけるα2キメリンの働きが、おとなでの脳機能を左右する (2015)
  • Author(s): 岩田亮平、岩里琢治
  • Journal Title: 化学と生物 Volume: 53 Pages: 568-570
  • NAID: 130005405063
• [Presentation] 赤ん坊の脳の中での神経回路の発達 ―マウス遺伝学と二光子イメージングを用いた研究 (2019)
  • Author(s): 岩里琢治
  • Organizer: 第18回BIRD脳科学セミナー
  • Invited
• [Presentation] In vivo imaging of barrel cortex layer 4 during thalamocortical reorganization (2018)
  • Author(s): Takuji Iwasato
  • Organizer: 遺伝研研究会2018 “Circuit construction in the mammalian brain”
  • Invited
• [Presentation] In vivo imaging of barrel cortex circuit refinement in neonates (2018)
  • Author(s): Takuji Iwasato
  • Organizer: Spontaneous Activity in Brain Development (SPONT 2018)
  • Int'l Joint Research / Invited
• [Presentation] In vivo functional and morphological imaging of neural circuit refinement in the neonatal barrel cortex (2018)
  • Author(s): Takuji Iwasato
  • Organizer: CSHA(Cold Spring Harbor Asia) on Neuroscience 2018
  • Int'l Joint Research / Invited
• [Presentation] 新生仔マウスのバレル皮質神経回路発達のin vivoイメージング(In vivo imaging of barrel cortex circuit refinement in neonates) (2018)
  • Author(s): 岩里琢治
  • Organizer: 第41回日本神経科学大会
  • Invited
• [Presentation] マウスで迫る「赤ん坊の脳の発達のしくみ」 (2018)
  • Author(s): 岩里琢治
  • Organizer: JT生命誌研究館・国立遺伝学研究所共同企画シンポジウム
  • Invited
• [Presentation] 哺乳類中枢神経系における神経回路形成の遺伝学的解析 (2017)
  • Author(s): 岩里琢治
  • Organizer: 新潟大学脳研究所共同研究合同セミナー
  • Place of Presentation: 新潟市
  • Year and Date: 2017-03-11
  • Invited
• [Presentation] マウス遺伝学を用いた体性感覚系神経回路発達の解析 (2017)
  • Author(s): 岩里琢治
  • Organizer: 新潟大学脳研究所共同研究合同セミナー
  • Invited
• [Presentation] Unveiling the mechanisms of neuronal circuit refinement in the neonatal mouse barrel Cortex (2017)
  • Author(s): Takuji Iwasato
  • Organizer: Academia Sinica Symposium
  • Int'l Joint Research / Invited
• [Presentation] マウス大脳皮質の生後発達期神経回路リモデリング (2017)
  • Author(s): 岩里琢治
  • Organizer: 第12回「認識と形成」研究会
  • Invited
• [Presentation] 生後発達期の大脳皮質リモデリング (2017)
  • Author(s): 岩里琢治
  • Organizer: 生理学研究所研究会「大脳皮質回路の機能原理を探る」
  • Invited
• [Presentation] マウス大脳皮質の生後発達期神経回路リモデリング (2017)
  • Author(s): 岩里琢治
  • Organizer: 第5回「幸福脳」研究会（名古屋大学・慶応大学合同ミーティング）
  • Invited
• [Presentation] In vivo imaging of neonatal mouse barrel cortex (2016)
  • Author(s): T. Iwasato.
  • Organizer: Dart Neuroscience Seminar Series at TSRI
  • Place of Presentation: Ｓａｎ Ｄｉｅｇｏ
  • Year and Date: 2016-11-18
  • Int'l Joint Research / Invited
• [Presentation] 新生仔大脳皮質における神経回路スクラップ&ビルド (2016)
  • Author(s): 岩里琢治
  • Organizer: スクラップ&ビルドによる脳機能の動的制御シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2016-09-08
  • Invited
• [Presentation] 独自の二光子イメージング技術を用いた、新生仔マウス大脳皮質における神経回路発達の解析 (2016)
  • Author(s): 岩里琢治
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 藤沢市
  • Year and Date: 2016-09-07
  • Invited
• [Presentation] In vivo imaging of neonatal mouse barrel cortex (2016)
  • Author(s): T. Iwasato.
  • Organizer: シンポジウム”Circuit Construction in the Mammalian Brain”
  • Place of Presentation: Ｏｓａｋａ
  • Year and Date: 2016-08-08
  • Int'l Joint Research / Invited
• [Presentation] In vivo imaging of neural circuit formation in the neonatal mouse cortex. (2015)
  • Author(s): T. Iwasato.
  • Organizer: 遺伝研研究会Circuit Construction in the Mammalian Brain
  • Place of Presentation: 三島市
  • Year and Date: 2015-12-06
  • Invited
• [Presentation] 新生仔マウス大脳皮質における神経回路形成のin vivoイメージング (2015)
  • Author(s): 岩里琢治
  • Organizer: 認識と形成研究会2015
  • Place of Presentation: Ｏｋｉｎａｗａ
  • Year and Date: 2015-09-20
  • Invited
• [Presentation] 新生仔バレル皮質における回路発達の二光子イメージング (2015)
  • Author(s): 岩里琢治
  • Organizer: 研究戦略ワークショップ「Strategy for Neuroscience 2015」
  • Place of Presentation: Ｍａｃｈｉｄａ
  • Year and Date: 2015-09-04
  • Invited
• [Presentation] In vivo Imaging of Circuit Refinement in Cortical Layer 4 of Neonatal Mouse. (2015)
  • Author(s): T. Iwasato.
  • Organizer: 第38回日本神経科学大会（シンポジウム）
  • Place of Presentation: Ｙｏｋｏｈａｍａ
  • Year and Date: 2015-07-28
  • Invited
• [Patent(Industrial Property Rights)] ベクターシステム、遺伝子発現方法、標的遺伝子ノックアウト方法、標的遺伝子ノックダウン方法、標的遺伝子編集方法、及び遺伝子発現キット (2016)
  • Inventor(s): 岩里琢治、水野秀信、羅ブンジュウ、岩田亮平
  • Industrial Property Rights Holder: 情報・システム研究機構
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-094814
  • Filing Date: 2016-05-10