Molecular network and physiological function of glutamatergic tripartite synapse in the cerebellum
| Project/Area Number |
15H04274
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Neurochemistry/Neuropharmacology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
高雄 啓三 富山大学, 研究推進機構 研究推進総合支援センター, 教授 (80420397)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
AGETA NATSUMI (ISHIHARA NATSUMI) 名古屋大学, 大学院理学研究科, 講師 (60547561)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
|
|---|
| Keywords | シナプス / 細胞骨格 / 足場 / セプチン / 認知機能 / マウス / 電子顕微鏡 / 海馬歯状回 / 空間弁別障害 / ライブイメージング / グリア突起 / セプチン細胞骨格 / グリア / グルタミン酸代謝 / 足場蛋白質 / tripartiteシナプス / 遺伝子改変マウス / グルタミン酸受容体 / 小脳 / スパイン / グルタミン酸トランスポーター / 運動学習 |
|---|
| Outline of Final Research Achievements |
The perforant path (pp)-granule cell (GC) synapse is poorly characterized at molecular/subcellular level. Here we show that mice that lack a pan-neuronal subunit of the septin cytoskeleton, pass hippocampus-dependent tasks. However, they consistently underperform in specific tasks that require discrimination among distinct spatial contexts. Hippocampal dentate gyrus (DG) neuron-selective supplementation of the septin subunit restores their performance, while the local depletion recapitulates the defects in wild-type mice. EM morphometry of three major hippocampal subregions shows normal synapse density, PSD area, and spine volume, but reveals a significant scarcity of spine apparatus that is most severe in the pp-GC synapse. Live imaging of septin-depleted primary cultured DG neurons reveals low frequency in ER entry from dendritic shafts into spines. These and other findings indicate a septin-mediated postsynaptic mechanism required in pp-GC synapses for spatial pattern separation.
|
Report
(4 results)
Research Products
(22 results)
-
-
-
[Journal Article] A CDC42EP4/septin-based perisynaptic glial scaffold facilitates glutamate clearance.2015
Author(s)
Ageta-Ishihara N, Yamazaki M, Konno K, Nakayama H, Abe M, Hashimoto K, Nishioka T, Kaibuchi K, Hattori S, Miyakawa T, Tanaka K, Huda F, Hirai H, Hashimoto K, Watanabe M, Sakimura K, Kinoshita M.
-
Journal Title
Nat Commun.
Volume: 6
Issue: 1
Pages: 10090-10090
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Spatial discrimination defects caused by chronic, systemic loss of a septin subunit is recapitulated by its acute, local depletion in the hippocampal dentate gyrus.2016
Author(s)
Ageta-Ishihara N, Yamada T, Y. Suzuki Y, A. Sawada A, Takao K, Miyakawa T, Okamura M, Bito H, Inokuchi K, Kinoshita M.
Organizer
Integrative Neural Network Linking Multiple Brain Areas for Behavioral Adaptation
Place of Presentation
京都
Year and Date
2016-03-03
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
