Analysis of implantaion process by using Sox17 heterozygous mouse

• Project/Area Number: 15H04282
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KANAI Masami 東京医科歯科大学, 統合研究機構, 教授 (70321883)
• Co-Investigator(Kenkyū-buntansha): 平手 良和 東京医科歯科大学, 統合研究機構, 講師 (70342839)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: 着床 / 不妊 / マウス / Sox17 / 着床不全 / 女性不妊 / 着床障害

Outline of Final Research Achievements

Few basic and systematic research has been done for maternal implantation process of fertilized embryo which is considered to be the factor in the pregnancy failure. In this study, we have done the pathological analysis by using female Sox17 heterozygous mouse. From the result of microarray analysis using endometrial epithelium from female Sox17 heterozygous mouse, we have selected that the gene group in which the signal ratio was significantly decreased as compared with the wild type.

Research Products

• [Int'l Joint Research] Utha Univerisity(米国)
• [Int'l Joint Research] School of Medicine/Universoty of Utha(米国)
• [Journal Article] Sox17 is Essential for Proper Formation of the Marginal Zone of Extraembryonic Endoderm Adjacent to a Developing Mouse Placental Disk. (2018)
  • Author(s): Igarashi Hitomi, Uemura Mami, Hiramatsu Ryuji, Hiramatsu Ryuto, Segami Saki, Pattarapanawan Montri, Hirate Yoshikazu Yoshimura Yuki, Hashimoto Haruo, Higashiyama Hiroki, Sumitomo Hiroyuki, Kurohmaru Masamichi, Saijoh Yukio, Suemizu Hiroshi, Kanai-Azuma Masami, Kanai Yoshiakira.
  • Journal Title: Biology of Reproduction Volume: -
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Maintenance of hematopoietic stem and progenitor cells in fetal intra-aortic hematopoietic clusters by the Sox17-Notch1-Hes1 axis. (2018)
  • Author(s): Saito K, Nobuhisa I, Harada K, Takahashi S, Anani M, Lickert H, Kanai-Azuma M, Kanai Y, Taga T.
  • Journal Title: Exp Cell Res Volume: 365 Issue: 1 Pages: 145-155
  • DOI: 10.1016/j.yexcr.2018.02.014
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Embryonic cholecystitis and defective gallbladder contraction in the Sox17-haploinsufficient mouse model of biliary atresia. (2017)
  • Author(s): Higashiyama H, Ozawa A, Sumitomo H, Uemura M, Fujino K, Igarashi H, Imaimatsu K, Tsunekawa N, Hirate Y, Kurohmaru M, Saijoh Y, Kanai-Azuma M, Kanai Y.
  • Journal Title: Development Volume: 144 Issue: 10 Pages: 1906-1917
  • DOI: 10.1242/dev.147512
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Essential roles of L-type amino acid transporter 1 in syncytiotrophoblast development by presenting fusogenic 4F2hc. (2017)
  • Author(s): Ohgaki R, Ohmori T, Hara S, Nakagomi S, Kanai-Azuma M, Kaneda-Nakashima K, Okuda S, Nagamori S, Kanai Y.
  • Journal Title: Mol Cell Biol. Volume: 印刷中 Issue: 11
  • DOI: 10.1128/mcb.00427-16
  • Peer Reviewed / Open Access
• [Journal Article] Embryonic cholecystitis and defective gallbladder contraction in the Sox17-haploinsufficient model of biliary atresia. (2017)
  • Author(s): Higashiyama H, Ozawa A, Sumitomo H, Uemura M, Fujino K, Igarashi H, Imaimatsu K, Tsunekawa N, Hirate Y, Kurohmaru M, Saijoh Y, Kanai-Azuma M, Kanai Y.
  • Journal Title: Development Volume: 印刷中
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Mouse Sox17 haploinsufficiency leads to female subfertility due to impared implantation. (2016)
  • Author(s): Hirate Y, Suzuki H, Kawasumi M, Takase HM, Igarashi H, Naquet P, Kanai Y, Kanai-Azuma M.
  • Journal Title: Sci Rep Volume: - Issue: 1 Pages: 24171-24171
  • DOI: 10.1038/srep24171
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Fate mapping of gallbladder progenitors in posteroventral foregut endoderm of mouse early somite-stage embryos (2015)
  • Author(s): Uemura M, Igarashi H, Ozawa A, Tsunekawa N, Kurohmaru M, Kanai-Azuma M, Kanai Y.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 5 Pages: 587-591
  • DOI: 10.1292/jvms.14-0635
  • NAID: 130005071872
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Sox17 heterozygous mutant females are defective in implantation (2017)
  • Author(s): Hirate Yoshikazu, Hayakawa Kana, Toyomura Yuga, Igarashi Hitomi, Miura Kento, Kanai Yoshiakira, Kanai-Azuma Masami
  • Organizer: Annual Meeting of the Japanese Society of Developmental Biologists 50th
  • Int'l Joint Research
• [Presentation] Sox17ヘテロ変異着床不全マウスにおける子宮上皮遺伝子の発現変化 (2017)
  • Author(s): 平手良和、早川佳那、豊村友賀、五十嵐瞳、三浦健人、金井克晃、金井正美
  • Organizer: 第64回日本実験動物学会
• [Presentation] Sox17ヘテロ変異雌マウスの子宮における着床不全のメカニズム解析 (2017)
  • Author(s): 早川佳那、平手良和、上村麻実、三浦健人、金井克晃、金井正美
  • Organizer: 第160回日本獣医学会
• [Presentation] マウス子宮上皮における転写因子Sox17の発言は着床の成立に必須である (2017)
  • Author(s): 平手良和、早川佳那、中野有紀、上村麻実、三浦健人、金井克晃、金井正美
  • Organizer: 第40回日本分子生物学会
• [Presentation] 着床不全モデルとしてのSox17変異マウスの解析 (2016)
  • Author(s): 平手良和、金井正美
  • Organizer: 第39回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
  • Invited
• [Presentation] SOX17 のマウス心内膜前駆細胞での発現と心筒形成過程における機能 (2016)
  • Author(s): 佐波理恵、北島桂子、Lucille Rainbow、Sylvia Engert、石田秀和、Ioannis Kokkinopoulos、金井克晃、金井正美、Peter Koopman、目野主税、John Kenny、Heiko Lickert、澤芳樹、相賀裕美子、鈴木健、八代健太
  • Organizer: 第39回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] 卵成熟と着床異常の疾患モデル (2016)
  • Author(s): 鈴木仁美、金井正美
  • Organizer: 第57回日本組織細胞化学会
  • Place of Presentation: 杏林大学（東京都三鷹市）
  • Year and Date: 2016-09-03
  • Invited
• [Presentation] Sox17ヘテロ雌マウスは着床受容能の低下のために低妊性となる (2016)
  • Author(s): 平手良和、鈴木仁美、川澄みゆり、高瀬比菜子、五十嵐瞳、金井克晃、金井正美
  • Organizer: 第63回日本実験動物学会
  • Place of Presentation: ミューザ川崎（神奈川県川崎市）
  • Year and Date: 2016-05-18
• [Presentation] マウスキーバンクによるマウスの学内共有および共同研究推進の試み (2016)
  • Author(s): 高橋仁美、鈴木仁美、金井正美
  • Organizer: 第63回日本実験動物学会
  • Place of Presentation: ミューザ川崎（神奈川県川崎市）
  • Year and Date: 2016-05-18
• [Presentation] Sox17ヘテロ雌マウスは着床不全のために妊性の低下がおこる (2015)
  • Author(s): 平手良和、鈴木仁美、川澄みゆり、高瀬比菜子、五十嵐瞳、金井克晃、金井正美
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド (兵庫県 神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Soxヘテロ雌マウスの着床におけるハプロ不全 (2015)
  • Author(s): 平手良和、鈴木仁美、川澄みゆり、金井克晃、金井正美
  • Organizer: 第62回日本実験動物学会
  • Place of Presentation: 京都テレサ (京都府、京都市）
  • Year and Date: 2015-05-28
• [Presentation] A possible involvement of a dose-dependent Sox17 activity of uterine epithelia in mouse implantation processes (2015)
  • Author(s): Kanai-Azuma Masami, Yoshikazu Hirate, Miyuri Kawasumi, Hitomi Suzuki, Yoshiakira Kana
  • Organizer: 7th International Symposium Biology of Vertebrate Sex Determination
  • Place of Presentation: Kona Hawaii (USA)
  • Year and Date: 2015-04-13
  • Int'l Joint Research / Invited
• [Remarks] 東京医科歯科大学 疾患モデル動物解析学分野ホームページ
  • URL: http://www.tmd-cea.jp/eam/
• [Remarks]
  • URL: http://www.tmd-cea.jp/eam/
• [Remarks] 研究内容
  • URL: http://www.tmd-cea.jp/eam/