| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|
|---|
| Outline of Final Research Achievements |
Humanized animals, in which drug metabolism-related genes of experimental animals are replaced into those of humans, are considered to play a major role in predicting human-specific drug metabolism and safety. In this study, we aimed to generate humanized animals using rats that can collect blood over time and have abundant background data such as toxicity tests among experimental animals. Specifically, following studies were performed. 1) CYP3A gene cluster and UGT2 gene cluster, which are related in human-specific drug metabolism, were introduced into rats using mouse artificial chromosome technology. 2) The rat gene cluster corresponding to the above human cluster was desrupted using the genome editing technique. 3) By mating 1) and 2), a fully humanized model rats showing human-specific metabolic ability were successfully generated.
|
