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Establishing model mouse for anxiety disorders using a novel wild-derived heterogeneous stock

Research Project

Project/Area Number 15H04289
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionNational Institute of Genetics

Principal Investigator

Koide Tsuyoshi  国立遺伝学研究所, 系統生物研究センター, 准教授 (20221955)

Co-Investigator(Renkei-kenkyūsha) TANAVE Akira  大学共同利用機関法人情報・システム研究機構, 新領域融合研究センター, 融合プロジェクト特任研究員 (30749023)
TAKAHASHI Aki  筑波大学, 人間系, 助教 (30581764)
HORII Yasuyuki  国立遺伝学研究所, 系統生物研究センター, 博士研究員 (50601960)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywords野生マウス / 不安様行動 / 多因子疾患 / QTL解析 / ゲノム / 遺伝子マッピング / 能動的従順性 / 家畜化 / 選択交配 / マウス / 従順性 / 遺伝子 / マッピング / 量的形質 / ヘテロジニアスストック / 遺伝学 / 精神疾患 / モデル動物 / 遺伝子発現 / アウトブレッドストック / 行動 / 野生系統 / 多因子形質 / アウトブレッド
Outline of Final Research Achievements

A novel wild - derived heterogeneous stock (WHS) was established by genetically mixing with eight wild strains. We conducted selective breeding for active tameness to humans and selected and non - selected groups were successfully established. By conducting SNP analysis using genomic DNA on these selected and non-selected groups of mice, we performed genetic analysis for active tameness. We found genetic loci relating to active tameness on chromosome 11. In order to narrowing down the loci, we conducted comparative analysis of mouse and dog genome. We found that the data of the selected region showed high degree of overlap with the genomic regions which were selected during the evolution of dog breeds.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report

Research Products

(48 results)

All 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Open Access: 5 results,  Acknowledgement Compliant: 3 results) Presentation (36 results) (of which Int'l Joint Research: 7 results,  Invited: 3 results) Book (2 results) Remarks (1 results)

  • [Int'l Joint Research] University College London(United Kingdom)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Hierarchy in the home cage affects behaviour and gene expression in group-housed C57BL/6 male mice.2017

    • Author(s)
      Horii Y, Nagasawa T, Sakakibara H, Takahashi A, Tanave A, Matsumoto Y, Nagayama H, Yoshimi K, Yasuda MT, Shimoi K, Koide T.
    • Journal Title

      Scientific Reports

      Volume: 7 Pages: 6991-6991

    • DOI

      10.1038/s41598-017-07233-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Selective breeding and selection mapping using a novel wild-derived heterogeneous stock of mice revealed two closely-linked loci for tameness.2017

    • Author(s)
      Matsumoto, Y., Goto, T., Nishino, J., Nakaoka, H., Tanave, A., Takano-Shimizu, T., Mott, R. F., and Koide, T.
    • Journal Title

      Sci Rep.

      Volume: 7 Pages: 4607-4607

    • DOI

      10.1038/s41598-017-04869-1

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pup exposure facilitates retrieving behavior via the oxytocin neural system in female mice2017

    • Author(s)
      Okabe, S., Tsuneoka, Y., Takahashi, A., Ooyama, R., Watarai, A., Maeda, S., Honda, Y., Nagasawa, M., Mogi, K., Nishimori, K., Kuroda, M., Koide, T., Kikusui, T.
    • Journal Title

      Psychoneuroendocrinology.

      Volume: 79 Pages: 20-30

    • DOI

      10.1016/j.psyneuen.2017.01.036

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Automated Estimation of Mouse Social Behaviors Based on a Hidden Markov Model.2017

    • Author(s)
      Arakawa T, Tanave A, Takahashi A, Kakihara S, Koide T, Tsuchiya T.
    • Journal Title

      Methods in Molecular Biology.

      Volume: 1552 Pages: 185-197

    • DOI

      10.1007/978-1-4939-6753-7_14

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Caspr3-Deficient Mice Exhibit Low Motor Learning during the Early Phase of the Accelerated Rotarod Task.2016

    • Author(s)
      Hirata H, Takahashi A, Shimoda Y, Koide T.
    • Journal Title

      PLOS ONE

      Volume: 11

    • DOI

      10.1371/journal.pone.0147887

    • NAID

      120007129617

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cell adhesion molecule Caspr3 is expressed in the mouse basal ganglia during early postnatal stages.2016

    • Author(s)
      Hirata, H., Umemori, J., Yoshioka, H., Koide, T., Watanabe, K., Shimoda, Y.
    • Journal Title

      Journal of Neuroscience Research

      Volume: 94 Pages: 74-89

    • DOI

      10.1002/jnr.23670

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mapping of genetic factors that elicit intermale aggressive behavior on Mouse Chromosome 15: intruder effects and the complex genetic basis2015

    • Author(s)
      Takahashi, A., Sugimoto, H., Kato, S., Shiroishi, T. and Koide, T.
    • Journal Title

      PLoS ONE

      Volume: 10(9): e0137764 Pages: 1-20

    • DOI

      10.1371/journal.pone.0137764

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Glutamate input in the dorsal raphe nucleus as a determinant of escalated aggression in male mice.2015

    • Author(s)
      Takahashi, A., Lee, R.X., Iwasato, T., Itohara, S., Arima, H., Bettler, B., Miczek, K.A., Koide, T.
    • Journal Title

      The Journal of Neuroscience

      Volume: 35 Pages: 6452-6463

    • NAID

      120007130264

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ヒトになつくマウスの行動・生理・神経および遺伝的基盤2017

    • Author(s)
      小出剛
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 選択交配マウスを用いて明らかにする動物家畜化の遺伝的基盤2017

    • Author(s)
      小出剛
    • Organizer
      日本畜産学会第124回大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] マウスにおけるテイム行動の遺伝学的・行動学的および神経学的基盤の解明に向けて2017

    • Author(s)
      小出剛
    • Organizer
      第27回日本行動神経内分泌研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] RNA-seqを用いたマウスの従順性に関わる遺伝的基盤の解明2017

    • Author(s)
      松本悠貴、中岡博史、永山博通、小出剛
    • Organizer
      第64回日本実験動物学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 長鎖一本鎖DNAを用いたリピート配列改変マウスの作製2017

    • Author(s)
      吉見一人、田邉彰、今井悠二、二瓶基子、小出剛
    • Organizer
      第64回日本実験動物学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Neurobehavioral study for relationship between sociability and tameness in domesticated mice2017

    • Author(s)
      Nagayama H, Matsumoto Y, Koide T
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Behavioral analysis of fear in PACAP over-expression mice2017

    • Author(s)
      Tanave A, Koide T
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] PACAP遺伝子改変マウスにおける情動行動および恐怖記憶学習の解析2017

    • Author(s)
      田邉彰、小出剛
    • Organizer
      第47回日本神経精神薬理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 野生由来マウス集団の選択交配による高度な従順化とその関連遺伝子座解析2017

    • Author(s)
      小出剛、永山博通、後藤達彦、松本悠貴
    • Organizer
      第77回日本動物心理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マウスの能動的従順性(人に対する接触行動)に関わる行動特性および神経基盤の解析2017

    • Author(s)
      永山博通、松本悠貴、小出剛
    • Organizer
      第77回日本動物心理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Hunting genetic factors associated with high anxiety-like behavior in wild mouse strain2016

    • Author(s)
      Koide T.
    • Organizer
      第46回日本神経精神薬理学会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Genetic variation and expression diversity of pituitary adenylate cyclase-activating polypeptide (PACAP) gene in mouse2016

    • Author(s)
      Koide T., Tanave A.
    • Organizer
      30th CINP World Congress of Neuropsychopharmacology
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] High expression of PACAP gene and the molecular mechanism found in wild-mouse strain showing elevated anxiety-like behavior2016

    • Author(s)
      Tanave A., Koide T., Sumiyama, K., Takahashi, A.
    • Organizer
      30th CINP World Congress of Neuropsychopharmacology
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Expression diversity and genetic variation of pituitary adenylate cyclase-activating polypeptide (PACAP) gene in mouse2016

    • Author(s)
      Tanave, A., Koide T.
    • Organizer
      The 39th Annual Meeting of the Japan Neuroscience Society
    • Related Report
      2016 Annual Research Report
  • [Presentation] Behavioral and neurological bases of tameness in selection groups of wild-derived heterogeneous stock2016

    • Author(s)
      Nagayama, H., Matsumoto, Y., Goto, T., Koide T.
    • Organizer
      The 39th Annual Meeting of the Japan Neuroscience Society
    • Related Report
      2016 Annual Research Report
  • [Presentation] 長鎖一本鎖オリゴを用いたノックイン動物作製法の開発2016

    • Author(s)
      吉見一人、今井悠二、田邉彰、真下知士、小出剛
    • Organizer
      The 88th Annual Meeting of the Genetics Society of Japan
    • Related Report
      2016 Annual Research Report
  • [Presentation] Selection mappingおよび関連解析によるマウス従順性関連遺伝領域の同定2016

    • Author(s)
      松本悠貴、西野譲、中岡博史、後藤達彦、小出剛
    • Organizer
      The 88th Annual Meeting of the Genetics Society of Japan
    • Related Report
      2016 Annual Research Report
  • [Presentation] 野生由来マウスヘテロジニアスストックの選択交配によりみられる従順性の解析2016

    • Author(s)
      永山博通、松本悠貴、後藤達彦、小出剛
    • Organizer
      The 63rd Annual Meeting of Japanese Association for Animal Science
    • Related Report
      2016 Annual Research Report
  • [Presentation] 野生ハツカネズミの下垂体アデニル酸シクラーゼ活性化ポリペプチド(PACA)遺伝子における正の自然選択2016

    • Author(s)
      田邉彰、小出剛
    • Organizer
      The 63rd Annual Meeting of Japanese Association for Animal Science
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウスの従順性行動に関わる遺伝子座とイヌの家畜化との関連2016

    • Author(s)
      松本悠貴、西野譲、後藤達彦、小出剛
    • Organizer
      The 63rd Annual Meeting of Japanese Association for Animal Science
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウス・ラットにおける長鎖一本鎖オリゴを用いた効率的なノックイン2016

    • Author(s)
      吉見一人、今井悠二、田邉彰、真下知士、小出剛
    • Organizer
      The 63rd Annual Meeting of Japanese Association for Animal Science
    • Related Report
      2016 Annual Research Report
  • [Presentation] Establishing new mouse resource, wild-derived heterogeneous stock, which is useful for genetic analysis of tameness and other complex traits.2015

    • Author(s)
      Koide, T., Matsumoto, Y., Nakaoka, H., Nishino, J., Goto, T.
    • Organizer
      29th International Mammalian Genome Conference
    • Place of Presentation
      Yokohama
    • Year and Date
      2015-11-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CRISPR/Cas9-mediated plasmid knock-in and replacement of genomic region with single stranded oligonucleotides in rodents2015

    • Author(s)
      Yoshimi, K., Kaneko, T., Koide, T., Mashimo, T.
    • Organizer
      29th International Mammalian Genome Conference
    • Place of Presentation
      Yokohama
    • Year and Date
      2015-11-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Combination of selective breeding and genome-wide SNP analysis revealed the genetic loci associated with tame behavior in mice2015

    • Author(s)
      Matsumoto, Y., Nakaoka, H., Nishino, J., Goto, T., Koide, T.
    • Organizer
      29th International Mammalian Genome Conference
    • Place of Presentation
      Yokohama
    • Year and Date
      2015-11-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Higher expression of PACAP gene is associated with altered behavioral and prolonged physiological responses to stress in wild-derived MSM mice2015

    • Author(s)
      Tanave, A., Takahashi, A., Sumiyama, K., Koide, T.
    • Organizer
      29th International Mammalian Genome Conference
    • Place of Presentation
      Yokohama
    • Year and Date
      2015-11-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 野生由来ヘテロジニアスストックを用いた遺伝学的解析の試み2015

    • Author(s)
      小出剛、後藤達彦、松本悠貴
    • Organizer
      第87回日本遺伝学会大会
    • Place of Presentation
      仙台
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 選択交配およびゲノムワイド遺伝解析によるマウスの従順性行動に関わる遺伝子座の同定2015

    • Author(s)
      松本悠貴、中岡博史、西野穣、後藤達彦、小出剛
    • Organizer
      第87回日本遺伝学会大会
    • Place of Presentation
      仙台
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 野生由来ヘテロジニアスストックマウスの選択交配によりみられる従順性と不安、新奇探索性、社会生との関連2015

    • Author(s)
      永山博通、松本悠貴、後藤達彦、小出剛
    • Organizer
      第23回日本行動神経内分泌研究会
    • Place of Presentation
      仙台
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] A series of behavioral analyses to reveal behavioral and psychological basis underlying tameness in selected population of wild-derived heterogeneous stock (WHS) of mice2015

    • Author(s)
      永山博通、松本悠貴、後藤達彦、小出剛
    • Organizer
      日本動物心理学会 第75回大会
    • Place of Presentation
      東京
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] Differences of sensitivity to anxiolytic effect of diazepam in wild-derived mouse strains2015

    • Author(s)
      Koide, T., Imai, Y., Hanzawa, N.
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] PACAP遺伝子発現によるストレス関連行動の調節の遺伝学的解析2015

    • Author(s)
      田邉彰、高橋阿貴、隅山健太、小出剛
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 雄マウスの集団飼育下における社会的順位が海馬の神経新生に及ぼす影響2015

    • Author(s)
      堀井康行、長澤達弘、田邉彰、高橋阿貴、下位香代子、小出剛
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Finding the Genetic Loci Associated with Tameness Using the Combination Method of Selective Breeding and Computer Simulation in Mice2015

    • Author(s)
      Matsumoto, Y., Nishino, J., Goto, T., Koide, T.
    • Organizer
      Vth International Wildlife Management Congress 2015
    • Place of Presentation
      札幌
    • Year and Date
      2015-07-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 集団飼育下で社会的順位が最下位の雄マウスは,最上位の個体と比べて不安様・うつ様形質を示す2015

    • Author(s)
      堀井康行、長澤達弘、田邉彰、高橋阿貴、下位香代子、小出剛
    • Organizer
      第62回日本実験動物学会総会
    • Place of Presentation
      京都
    • Year and Date
      2015-05-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] MSM/Msマウスの不安様行動関連遺伝子の同定とその機能解析2015

    • Author(s)
      田邉彰、高橋阿貴、隅山健太、小出剛
    • Organizer
      第62回日本実験動物学会総会
    • Place of Presentation
      京都
    • Year and Date
      2015-05-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 選択交配およびコンピュータシミュレーションを用いた従順性行動に関わる遺伝子座の同定2015

    • Author(s)
      松本悠貴、西野穣、後藤達彦、小出剛
    • Organizer
      第62回日本実験動物学会総会
    • Place of Presentation
      京都
    • Year and Date
      2015-05-28
    • Related Report
      2015 Annual Research Report
  • [Book] マウス表現型解析スタンダード2016

    • Author(s)
      伊川 正人、若菜 茂晴、高橋 智
    • Total Pages
      347
    • Publisher
      羊土社
    • ISBN
      9784758101981
    • Related Report
      2016 Annual Research Report
  • [Book] 個性は遺伝子で決まるのか2015

    • Author(s)
      小出剛
    • Total Pages
      192
    • Publisher
      ベレ出版
    • Related Report
      2015 Annual Research Report
  • [Remarks] 家畜動物はなぜ人になつくのか~人に近づくマウスをつくり遺伝のしくみを解明~

    • URL

      https://www.nig.ac.jp/nig/ja/2017/07/research-highlights_ja/20170705.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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