| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Outline of Final Research Achievements |
Cancer stem cells (CSCs) reside in a microenvironment, niche. Elucidation of the CSC niche helps develop new cancer therapy. We have identified a CSC niche-mimicking polymer from 376 synthetic polymers and identified an iron-carrier transferrin (Tf) which binds to it. In the tumor formed in the mouse brain following transplantation of rat C6 glioma CSCs, iron was found to be stored in tumor-associated macrophages (TAMs), suggesting contribution of iron-storing TAMs to cancer progression. We further demonstrated that CSCs recruit monocytes and induce their differentiation into TAMs. Since PpIX, a fluorescent metabolite of 5-ALA, accumulates in glioma cells, 5-ALA is used for fluorescence-guided surgical resection. We indicated that CSCs exhibit low PpIX accumulation among glioma cells via nonfluorescent conversion by iron incorporated by Tf receptor, suggesting that CSCs escape from surgical resection. We propose that glioma CSCs have a capacity to construct self-advantageous niche.
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