Grant-in-Aid for Scientific Research (B)
Cancer tissues are composed of very heterogenous cell populations, including cancer stem cells, progenitor cancer cells and differentiated cancer cells. It is thus important to clarify the characteristics of each cell type at single cell levels. The aim of this study to clarify the molecular mechanisms how cancer stem cells are maintained in the caner stem cell niche by analyzing transcriptome at single cell levels. We have clarified the semaphorin-neuropilin (NP) signal plays critical roles for breast cancer stem cells. We sorted NP-positive and control NP-negative cell population derived from breast cancer patient samples and analyzed transcriptome at single cell levels by using the cutting-edge technologies. We found that NP-positive cell population show strong variance in mRNA expression patterns than NP-negative cell population. These findings suggest that NP-positive cells are composed of more heterogenous cell populations with clear hierarchy than NP-negative cells.
Attribution of KAKENHI